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![Knockout Validated: alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - Western blot shows lysates of HepG2 human liver carcinoma cells parental cell line and AFP knockout (KO) HepG2 cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # NBP2-32914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP at approximately 74 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C3-Knockout-Validated-NBP2-32914-img0009.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - Western blot shows lysates of HepG2 human liver carcinoma cells parental cell line and AFP knockout (KO) HepG2 cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # NBP2-32914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP at approximately 74 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - Formalin-fixed, paraffin-embedded human fetal liver stained with AFP MAb (C3).](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C3-Immunohistochemistry-Paraffin-NBP2-32914-img0001.jpg)
![Flow (Intracellular): alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - An intracellular stain was performed on HepG2 cells with alpha-Fetoprotein/AFP Antibody [C3] NBP2-34577AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image using the Alexa Fluor 700 Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C3-Flow-Intracellular-NBP2-32914-img0010.jpg)