![Knockout Validated: alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - Western blot shows lysates of HepG2 human liver carcinoma cells parental cell line and AFP knockout (KO) HepG2 cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # NBP2-32914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP at approximately 74 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C3-Knockout-Validated-NBP2-32914-img0009.jpg "Knockout Validated: alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - Western blot shows lysates of HepG2 human liver carcinoma cells parental cell line and AFP knockout (KO) HepG2 cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # NBP2-32914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP at approximately 74 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.")
| Reactivity | Hu, Po, Ca, Pm, Bv(-), Mu(-), Rt(-)Species Glossary |
| Applications | ELISA, Flow, IHC, KO |
| Clone | C3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34577) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Alpha feto protein (AFP) Purified from serum of a hepatoma patient (Uniprot: P02771) |
| Localization | Cytoplasmic |
| Marker | Hepatocellular/Germ Cell Tumor Marker |
| Specificity | It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This monoclonal antibody is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AFP |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. Use in Sandwich -ELISA reported in scientific literature (PMID:31921529). |
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| Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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| Publication using NBP2-32914 | Applications | Species |
|---|---|---|
| Dadfar S, Sekula-Neuner S, Trouillet V, et al. Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP) Beilstein J. Nanotechnol. 2019-12-16 [PMID: 31921529] (S-ELISA) | S-ELISA |
Secondary Antibodies |
Isotype Controls |
Research Areas for alpha-Fetoprotein/AFP Antibody (NBP2-32914)Find related products by research area.
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![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - Formalin-fixed, paraffin-embedded human fetal liver stained with AFP MAb (C3).](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C3-Immunohistochemistry-Paraffin-NBP2-32914-img0001.jpg "Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - Formalin-fixed, paraffin-embedded human fetal liver stained with AFP MAb (C3).")
![Flow (Intracellular): alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - An intracellular stain was performed on HepG2 cells with alpha-Fetoprotein/AFP Antibody [C3] NBP2-34577AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image using the Alexa Fluor 700 Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C3-Flow-Intracellular-NBP2-32914-img0010.jpg "Flow (Intracellular): alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - An intracellular stain was performed on HepG2 cells with alpha-Fetoprotein/AFP Antibody [C3] NBP2-34577AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image using the Alexa Fluor 700 Azide and BSA Free format of this antibody.")
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