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NB600-1015

Podoplanin Antibody (8.1.1) - BSA Free

(1 Review)

(21 Publications)

0.1 ml

NB600-1015

Western Blot: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Podoplanin is differentially expressed in primary podocytes relative to primary PECs after six passages in culture (arrow), consistent with mRNA expression analysis. Lysates of isolated glomeruli are used as positive control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22529955) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- at 5 ug/ml overnight at 4C and detected with an anti-Golden Syrian hamster DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin and these are cells of a malignant serosal tumor. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol (2014)

Western Blot: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Analysis of Podoplanin in mouse kidney tissue extract.

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Macrophages were isolated from wild-type (WT) mice treated with acetaminophen (APAP) for 3 hr. The cells were treated in vitro with either control IgG (Ctrl IgG) or an anti-podoplanin antibody (alpha-podoplanin Ab) before incubation with platelets. Immunofluorescence (IF) staining was performed to detect podoplanin on Macrophages and C-type lectin-like receptor 2 (Clec-2) on platelets. Scale bar, 25 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34110284/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana Schito.

Immunohistochemistry: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Podoplanin (8.1.1) antibody labeling (Green) of glomeruli from mouse kidney. Nuclei were counterstained with DAPI (Blue).

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver and gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol (2014)

Podolanin expressing on Mɸs mediates interactions with platelets.Mɸs were isolated from wild-type (WT) mice treated with acetaminophen (APAP) for 3 hr. The cells were treated in vitro with either control IgG (Ctrl IgG) or an anti-podoplanin antibody ( alpha -podoplanin Ab) before incubation with platelets. Immunofluorescence (IF) staining was performed to detect podoplanin on Mɸs and C-type lectin-like receptor 2 (Clec-2) on platelets. Scale bar, 25 μm.

Characterization of the primary PEC lines.PECs were negative for the endothelial marker von Willebrand factor (vWT, A) and for myofibroblast marker alpha-SMA (B). HUVECs (A') or human dermal fibroblasts (B') were used as positive controls. Polyclonal parietal cells were positive for claudin-1 (C) and caveolin-1 (D). C', D'. Negative controls were performed using isotype-matched irrelevant primary antibodies. E. A significantly lower expression of synaptopodin was observed in parietal cells expressed even after six passages compared to primary podocytes (E') or an immortalized podocyte cell line IHPC (E'). The findings were confirmed by SDS-page with subsequent immunoblotting using lysates of parietal cell cultures 2, 8 and 11 (F-H). Lysates of polyclonal primary podocyte cultures as well as of an immortalized podocyte cell line IHPC also showed expression of claudin-1 and calveolin-1. No expression of caveolin-1 was observed in Hel-1, GDM or Set-1 cell lines (G). Equal loading was verified by Ponceau S stain. H. Podocin expression is absent in primary PECs and down regulated in primary podocyte cells and in an immortalized podocyte cell line IHPC. I. Podoplanin is differentially expressed in primary podocytes relative to primary PECs after six passages in culture (arrow), consistent with mRNA expression analysis. Lysates of isolated glomeruli are used as positive control (H, I). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22529955), licensed under a CC-BY licence.

SMC2 (a) and SMC4 (b) in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 μm. (c) Localization of Nesprin-2 after siRNA mediated knockdown of SMC2 in COS7 cells. Staining was with SMC2 specific antibodies and mAb K20-478 for Nesprin-2. Bar, 5 μm. (d) Evaluation of the SMC2 knockdown. SMC2 fluorescence intensity was measured in the center of mitotic chromosomes. 10 siRNA treated cells and 12 control cells (control treatment) were analyzed (∗∗∗P value = 0.0001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29445399), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Mu, Hu(-)

Species Glossary

Reactivity: Mu, Hu(-)

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Golden Syrian Hamster Monoclonal

Host: Golden Syrian Hamster Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-1015AF350
Alexa Fluor 405	NB600-1015AF405
Alexa Fluor 488	NB600-1015AF488
Alexa Fluor 532	NB600-1015AF532
Alexa Fluor 594	NB600-1015AF594
Alexa Fluor 647	NB600-1015AF647
Alexa Fluor 700	NB600-1015AF700
Alexa Fluor 750	NB600-1015AF750
Allophycocyanin	NB600-1015APC
Biotin	NB600-1015B
DyLight 350	NB600-1015UV
DyLight 405	NB600-1015V
DyLight 488	NB600-1015G
DyLight 550	NB600-1015R
DyLight 594	NB600-1015DL594
DyLight 650	NB600-1015C
DyLight 680	NB600-1015FR
DyLight 755	NB600-1015IR
FITC	NB600-1015F
HRP	NB600-1015H
Janelia Fluor 549	NB600-1015JF549
Janelia Fluor 646	NB600-1015JF646
PE	NB600-1015PE
PerCP	NB600-1015PCP
mFluor Violet 450 SE	NB600-1015MFV450
mFluor Violet 500 SE	NB600-1015MFV500
mFluor Violet 610 SE	NB600-1015MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-1015SS
Azide and BSA Free	NBP2-80916

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NBP1-97035

Golden Syrian Hamster IgG Isotype Control

5 mg

Reactivity:

SyHa

Species Glossary

Reactivity: SyHa

Applications:

, ,

Applications: , ,

Host:

Golden Syrian Hamster

Host: Golden Syrian Hamster

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	NBP1-96834
FITC	NBP1-96872
HRP	NBP1-96965
Rhodamine	NBP1-96873
Texas Red	NBP1-96833

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NBP1-28115

KLRG1 Antibody (2F1) - Azide and BSA Free

0.25 mg

NBP1-28115

Flow Cytometry: KLRG1 Antibody (2F1) [NBP1-28115] - C57BL/6 mouse splenocytes were stained with Hamster Anti-Mouse KLRG1-FITC (SB Cat. 1807-02) and Mouse Anti-Mouse NK1.1-PE (SB Cat. No. 1805-09).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Golden Syrian Hamster Monoclonal

Host: Golden Syrian Hamster Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NBP1-28115AF405
Alexa Fluor 488	NBP1-28115AF488
Alexa Fluor 647	NBP1-28115AF647
Alexa Fluor 700	NBP1-28115AF700
Allophycocyanin	NBP1-28115APC
Biotin	NBP1-28117
FITC	NBP1-28116

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NBP1-28082

Ly49C Antibody (14B11) - Azide and BSA Free

0.5 mg

NBP1-28082

Flow Cytometry: Ly49C Antibody (14B11) [NBP1-28082] - Analysis of splenocytes by multiple staining.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Golden Syrian Hamster Monoclonal

Host: Golden Syrian Hamster Monoclonal

Conjugate:

Unconjugated

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