| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-59787SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93086-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94515-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05632-20ul | |||
![Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed using HRP-labeled secondary with DAB reagent followed by hematoxylin counterstaining. This antibody generated an expected strong nuclear staining in most of the cells in the glandular as well as the connective tissue. The cells showed some cytoplasmic signal also which was much weaker than the nuclear staining.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NB100-59787-img0018.jpg "Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed using HRP-labeled secondary with DAB reagent followed by hematoxylin counterstaining. This antibody generated an expected strong nuclear staining in most of the cells in the glandular as well as the connective tissue. The cells showed some cytoplasmic signal also which was much weaker than the nuclear staining.")
![Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-NB100-59787-img0017.jpg)
![Western Blot: PML Protein Antibody [NB100-59787] - Detection of Human PML on HeLa whole cell lysate using NB100-59787. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59788 and another CDC16/APC6 Ab.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NB100-59787-img0016.jpg)
![Western Blot: PML Protein Antibody [NB100-59787] - PML-RARa is a HIF-I+-transcriptional co-activator. HEK-293 cells transfected with stable mutants of HIF-1I+-or HIF-2I+-and increasing concentrations of PML-RARI+-. Image collected and cropped by Citeab from the following publication (HIF factors cooperate with PML-RAR +/- to promote acute promyelocytic leukemia progression and relapse. EMBO Mol Med (2014)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NB100-59787-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with PML Protein Antibody (NB100-59787) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PML-Protein-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-59787-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with PML Protein Antibody (NB100-59787) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/PML-Protein-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-59787-img0020.jpg)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PML used at a dilution of 1:500](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NB100-59787-img0015.jpg)
![Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Proximity-Ligation-Assay-NB100-59787-img0006.jpg)
![Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Proximity-Ligation-Assay-NB100-59787-img0014.jpg)
![Western Blot: PML Protein Antibody [NB100-59788] - Detection of Human PML on HeLa whole cell lysate using NB100-59788. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59787 and another PML antibody.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NB100-59788-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PML Protein Antibody [NB100-59788] - Detection of Human PML on HeLa whole cell lysate using NB100-59788. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59787 and another PML antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59788] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-NB100-59788-img0005.jpg)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP1-87783] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP1-87783-img0006.jpg "Immunohistochemistry-Paraffin: PML Protein Antibody [NBP1-87783] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.")
![Western Blot: PML Protein Antibody [NBP2-93086] - Western blot analysis of extracts of various cell lines, using PML antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP2-93086-img0001.jpg "Western Blot: PML Protein Antibody [NBP2-93086] - Western blot analysis of extracts of various cell lines, using PML antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s")
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-93086] - Immunofluorescence analysis of L929 cells using PML (isoform 5) Rabbit pAb (NBP2-93086) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93086-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-93086] - Immunofluorescence analysis of C6 cells using PML antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93086-img0002.jpg)
![Western Blot: PML Protein Antibody (SR1825) [NBP3-21728] - Western blot analysis using NBP3-21728 in 293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21728_rabbit-pml-protein-mab-sr1825-5720231595513.jpg "Western Blot: PML Protein Antibody (SR1825) [NBP3-21728] - Western blot analysis using NBP3-21728 in 293 cell lysate.")
![Western Blot: PML Protein Antibody [NBP2-98823] - Anti-PML Protein rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 48-98 kDa. Observed band size: 85 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP2-98823-img0003.jpg "Western Blot: PML Protein Antibody [NBP2-98823] - Anti-PML Protein rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 48-98 kDa. Observed band size: 85 kDa (We are unsure as to the identity of these extra bands).")
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-98823] - Immunofluorescence staining of PML Protein in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PML Protein polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98823-img0001.jpg)
![Immunoprecipitation: PML Protein Antibody [NBP2-98823] - PML Protein was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. 4 ul anti-PML Protein rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-PML Protein rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48-98 kDa. Observed band size: 85 kDa](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunoprecipitation-NBP2-98823-img0002.jpg)
![Western Blot: PML Protein Antibody [NBP3-18686] - Figure 2. Western blot analysis using the antibody directed against PML Protein Human embryonic kidney cells (293T) were transfected with an ER-tagged PML Protein/RAR fusion protein. Nuclear extracts (50 ug) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the antibody against PML Protein . The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML Protein/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML Protein protein.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP3-18686-img0001.jpg "Western Blot: PML Protein Antibody [NBP3-18686] - Figure 2. Western blot analysis using the antibody directed against PML Protein Human embryonic kidney cells (293T) were transfected with an ER-tagged PML Protein/RAR fusion protein. Nuclear extracts (50 ug) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the antibody against PML Protein . The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML Protein/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML Protein protein.")
![ELISA: PML Protein Antibody [NBP3-18686] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human PML Protein , crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.](http://images.novusbio.com/fullsize/PML Protein Antibody-ELISA-NBP3-18686-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-98822] - Immunofluorescence staining of PML Protein in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PML Protein polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98822-img0001.jpg "Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-98822] - Immunofluorescence staining of PML Protein in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PML Protein polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat.")
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP2-98822] - Immunochemical staining of human PML Protein in human lymph node with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-98822-img0003.jpg)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP2-98822] - Immunochemical staining of human PML Protein in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-98822-img0002.jpg)
![Western Blot: PML Protein Antibody [NBP2-94515] - Analysis of extracts of various cell lines, using PML Protein . Exposure time: 3min.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP2-94515-img0001.jpg "Western Blot: PML Protein Antibody [NBP2-94515] - Analysis of extracts of various cell lines, using PML Protein . Exposure time: 3min.")
![Western Blot: PML Protein Antibody [NBP2-19872] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP2-19872-img0001.jpg "Western Blot: PML Protein Antibody [NBP2-19872] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:5000.")
![Immunoprecipitation: PML Protein Antibody [NBP2-19872] - Immunoprecipitation of PML protein from A549 whole cell extracts using 5 ug of PML antibody [N1N2], N-term. Western blot analysis was performed using PML antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunoprecipitation-NBP2-19872-img0004.jpg)
![Western Blot: PML Protein Antibody [NBP3-05632] - Western blot analysis of extracts of various cells, using PML Protein antibody (NBP3-05632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP3-05632-img0001.jpg "Western Blot: PML Protein Antibody [NBP3-05632] - Western blot analysis of extracts of various cells, using PML Protein antibody (NBP3-05632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")