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![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***P<0.001. Data were expressed as mean +/- SEM. Three replicated experiments were carried out with the similar results. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.18199) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP2-16679-img0012.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***P<0.001. Data were expressed as mean +/- SEM. Three replicated experiments were carried out with the similar results. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.18199) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16679-img0011.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Rat liver. Glutamate Dehydrogenase stained by Glutamate Dehydrogenase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16679-img0014.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamate Dehydrogenase antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP2-16679-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody diluted at 1:500.Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16679-img0008.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Human breast cancer, using Glutamate Dehydrogenase antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16679-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Mouse kidney. Glutamate Dehydrogenase stained by Glutamate Dehydrogenase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16679-img0013.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] -Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GLUD1+ GLUD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16679_rabbit-polyclonal-glutamate-dehydrogenase-antibody-western-blot-28320248306..jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NB600-853] - Western blot analysis is shown using Glutamate Dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose. The blot was incubated with a 1:1,000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NB600-853-img0006.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NB600-853] - Western blot analysis is shown using Glutamate Dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose. The blot was incubated with a 1:1,000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![N/A: Glutamate Dehydrogenase Antibody [NB600-853]](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-N-A-NB600-853-img0005.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - The results of western blotting and RT-PCR assayA. shows Western blotting results, where the PDHA1 protein expression in the parental LNCaP cells is strongly positive, but its expression in the PDHA1 KO cells is almost negative with two different PDHA1 antibodies. Comparatively, the protein expression of the MPC1 and MPC2 is comparatively decreased and the protein expression of the GLUT1, HK2, LDHA, HIF1alpha, GLS1 and GLUD1 (Glutamate Dehydrogenase) is increased in the LNCaP PDHA1 KO cells. GAPDH was added as a loading control. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.10782) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54961-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - The results of western blotting and RT-PCR assayA. shows Western blotting results, where the PDHA1 protein expression in the parental LNCaP cells is strongly positive, but its expression in the PDHA1 KO cells is almost negative with two different PDHA1 antibodies. Comparatively, the protein expression of the MPC1 and MPC2 is comparatively decreased and the protein expression of the GLUT1, HK2, LDHA, HIF1alpha, GLS1 and GLUD1 (Glutamate Dehydrogenase) is increased in the LNCaP PDHA1 KO cells. GAPDH was added as a loading control. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.10782) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Glutamate Dehydrogenase Antibody [NBP1-54961] - GLS1 and GLUD1 (Glutamate Dehydrogenase) are over-expressed in prostate cancers and associated with poor survival. Immunohistochemical staining of GLUD1 in human prostate cancers with Gleason score 4 (D), 7 (E). and 9 (F). Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.10782) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-NBP1-54961-img0006.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - HT1080 cell lysate, concentration 1ug/ml.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54961-img0001.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - lanes 5: rat kidney cordex lanes 6: rat kidney proximal tubules prepped from cortex lanes 7: LLCPK-F+ pig kidney proximal tubule tissue culture lysate lanes 8: rat brain supernatant](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54961-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-54961] - Human colon tissue.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-54961-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-54961] - Human kidney, renal tubular epithelium in cortex.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-54961-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-54961] - Human pancreas tissue](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-54961-img0005.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54962] - Sample Type: Human Adult Placenta Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54962-img0012.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54962] - Sample Type: Human Adult Placenta Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-54962] - Human brain cortex.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-54962-img0004.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54962] - Human Fetal Liver Lysate, concentration 1ug/ml.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54962-img0001.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54962] - lanes 5: rat kidney cordex lanes 6: rat kidney proximal tubules prepped from cortex lanes 7: LLCPK-F+ pig kidney proximal tubule tissue culture lysate lanes 8: rat brain supernatant.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54962-img0007.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54962] - Sample Tissue: Human Fetal Liver, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54962-img0008.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54962] - Sample Type: Human Fetal Heart Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54962-img0009.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54962] - Sample Type: 293T Antibody Dilution: 1.0 ug/ml GLUD1 is supported by BioGPS gene expression data to be expressed in HEK293T](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54962-img0010.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54962] - Sample Type: Human Fetal Brain Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54962-img0011.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-54962] - Human pancreas tissue.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-54962-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-54962] - Human kidney, renal tubular epithelium in cortex.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-54962-img0003.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Western-Blot-NBP3-16582-img0005.jpg "Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunofluorescence analysis of NIH-3T3 cells using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Immunocytochemistry-Immunofluorescence-NBP3-16582-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Mouse kidney using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Immunohistochemistry-Paraffin-NBP3-16582-img0003.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Western-Blot-NBP3-16582-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Rat liver using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Immunohistochemistry-Paraffin-NBP3-16582-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Human esophageal using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Immunohistochemistry-Paraffin-NBP3-16582-img0002.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-68846-img0006.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-68846] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-68846-img0002.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A) and Mouse (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-68846-img0004.jpg)
![Western Blot: GLUD1/GLUD2 Antibody (HL2124) - Azide and BSA Free [NBP3-25484] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1+GLUD2 antibody [HL2124] (NBP3-25484) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25484_rabbit-glud1-glud2-mab-hl2124-azide-and-bsa-free-2120249432816.jpg "Western Blot: GLUD1/GLUD2 Antibody (HL2124) - Azide and BSA Free [NBP3-25484] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1+GLUD2 antibody [HL2124] (NBP3-25484) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GLUD1/GLUD2 Antibody (HL2124) - Azide and BSA Free [NBP3-25484] - GLUD1+ GLUD2 antibody [HL2124] detects GLUD1+ GLUD2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. GLUD1+ GLUD2 stained by GLUD1+ GLUD2 antibody [HL2124] (NBP3-25484) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25484_rabbit-glud1-glud2-mab-hl2124-azide-and-bsa-free-21202494081.jpg)
![Immunohistochemistry-Paraffin: GLUD1/GLUD2 Antibody (HL2124) - Azide and BSA Free [NBP3-25484] - GLUD1+ GLUD2 antibody [HL2124] detects GLUD1+ GLUD2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. GLUD1+ GLUD2 stained by GLUD1+ GLUD2 antibody [HL2124] (NBP3-25484) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25484_rabbit-glud1-glud2-mab-hl2124-azide-and-bsa-free-2120249393513.jpg)
![Western Blot: GLUD1/GLUD2 Antibody (HL2124) - Azide and BSA Free [NBP3-25484] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1+GLUD2 antibody [HL2124] (NBP3-25484) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25484_rabbit-glud1-glud2-mab-hl2124-azide-and-bsa-free-2120249342782.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: GLUD1/GLUD2 Antibody (HL2124) - Azide and BSA Free [NBP3-25484] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1+ GLUD2 antibody [HL2124] (NBP3-25484) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25484_rabbit-glud1-glud2-mab-hl2124-azide-and-bsa-free-2120249374999.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-54715] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG Lane 4: Human plasma Lane 5: Human Liver tissue Lane 6: Human Tonsil tissue](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP2-54715-img0004.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP2-54715] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG Lane 4: Human plasma Lane 5: Human Liver tissue Lane 6: Human Tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-54715] - Staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54715-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-54715] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-54715-img0001.jpg)
![Immunohistochemistry-Paraffin: GLUD1/GLUD2 Antibody (HL2125) - Azide and BSA Free [NBP3-25486] - GLUD1+ GLUD2 antibody [HL2125] detects GLUD1+ GLUD2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GLUD1+ GLUD2 stained by GLUD1+ GLUD2 antibody [HL2125] (NBP3-25486) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25486_rabbit-glud1-glud2-mab-hl2125-azide-and-bsa-free-2120249403870.jpg "Immunohistochemistry-Paraffin: GLUD1/GLUD2 Antibody (HL2125) - Azide and BSA Free [NBP3-25486] - GLUD1+ GLUD2 antibody [HL2125] detects GLUD1+ GLUD2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GLUD1+ GLUD2 stained by GLUD1+ GLUD2 antibody [HL2125] (NBP3-25486) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GLUD1/GLUD2 Antibody (HL2125) - Azide and BSA Free [NBP3-25486] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1+ GLUD2 antibody [HL2125] (NBP3-25486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25486_rabbit-glud1-glud2-mab-hl2125-azide-and-bsa-free-2120249422697.jpg)
![Immunohistochemistry-Paraffin: GLUD1/GLUD2 Antibody (HL2125) - Azide and BSA Free [NBP3-25486] - GLUD1+ GLUD2 antibody [HL2125] detects GLUD1+ GLUD2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. GLUD1+ GLUD2 stained by GLUD1+ GLUD2 antibody [HL2125] (NBP3-25486) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25486_rabbit-glud1-glud2-mab-hl2125-azide-and-bsa-free-212024939146.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody (SR1881) [NBP3-22240] - Western blot analysis using NBP3-22240 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22240_rabbit-glutamate-dehydrogenase-mab-sr1881-5720231564715.jpg "Western Blot: Glutamate Dehydrogenase Antibody (SR1881) [NBP3-22240] - Western blot analysis using NBP3-22240 in HepG2 cell lysate.")
![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-57114] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP2-57114-img0002.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP2-57114] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-57114] - Staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57114-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-54700] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-54700-img0001.jpg "Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-54700] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.")