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NB300-141

GFAP Antibody

(12 Reviews)

(102 Publications)

0.05 ml

NB300-141

Immunohistochemistry: GFAP Antibody [NB300-141] - Analysis of a rat cerebellum section stained with rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green and mouse monoclonal antibody to MeCP2, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 uM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).

Simple Western: GFAP Antibody [NB300-141] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - The inhibition of Kv1.3 channels induces neuroprotection against the toxic effects of glioma. Immunofluorescence analyses of Kv1.3 expression (red) on GFAP-positive cells (green) in coronal brain slices of GL261-bearing mice in peritumoral region; in the magnification (right) arrows indicate co-expression of Kv1.3 and GFAP in the same cell. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-25940-5) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: GFAP Antibody [NB300-141] - Mouse brain section, 20x magnification. Antibody at 1:1000. Detection with Polymer-HRP. IHC image submitted by a verified customer review.

Western Blot: GFAP Antibody [NB300-141] - Analysis of Rat brain lysate. Antibody at 1:5000. Specific band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP was observed.

Western Blot: GFAP Antibody [NB300-141] - Analysis of GFAP expression in whole rat cerebellum homogenate.

Western Blot: GFAP Antibody [NB300-141] - Analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50 kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Cultured Rat hippocampal neurons. ICC/IF image submitted by a verified customer review.

Immunohistochemistry: GFAP Antibody [NB300-141] - Xenografted mouse brain section: astocyte and human nuclei. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Frozen: GFAP Antibody [NB300-141] - Imaging of mouse brain (cortex), 20x magnification. IHC image submitted by a verified customer review.

EGFP+ neurons are positive for RABV antigen.Brains were collected from Cre reporter mice fifteen days post-infection, cryosectioned, and EGFP+ regions compared to cell-specific labeling, A) NeuN (blue, neuronal nuclei antibody, 20× fluorescence imaging), B) GFAP (blue, astrocyte antibody, 40× confocal imaging), or C) RABV P antigen (purple) and DAPI nuclear stain (blue, 63× confocal imaging). White arrows in (C) indicate regions positive for RABV P.

Increased astrocyte activation in IL-1beta-stimulated two-week old offspring is reduced by maternal LB supplementation. Representative images of fluorescence microscopy of claudin-5+ (location of the brain capillaries, red), GFAP+ astrocyte (green), and DAPI (nuclei, blue). Three to five sections per mouse were examined and at least three mice were examined in each group. Stronger than control SPF GFAP staining was observed around the blood vessel (see arrow) after IL-1beta insult. Maternal supplemented group (LB) with or without postnatal insult had GFAP levels similar to the control group indicating that LB supplementation prevented astrocyte activation around the BBB endothelium. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32424168), licensed under a CC-BY licence.

Effects of the repeated administration of NVP CXCR2 20 (NVP; 10 ug/5 ul; i.t.; 16 h and 1 h before CCI and then once a day for 7 days) on the protein levels of CXCR2, IBA1, GFAP, CXCL1, CXCL2, and CXCL3 proteins (A-I) in the spinal cord (A-F) and DRG (G-I) on the 7th day after CCI in rats. The data are presented as the mean fold changes relative to the control +/- SEM (5-6 samples per group). Intergroup differences were analyzed using ANOVA with Bonferroni's multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001 indicate differences vs. naive rats. #p < 0.05, indicate differences between V-treated and NVP-treated rats. CCI, chronic constriction injury; N, naive; V, vehicle; NVP, NVP CXCR2 20. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31616413), licensed under a CC-BY licence.

The inhibition of Kv1.3 channels induces neuroprotection against the toxic effects of glioma. (i) Immunofluorescence analyses of Kv1.3 expression (red) on GFAP-positive cells (green) in coronal brain slices of GL261-bearing mice in peritumoral region; in the magnification (right) arrows indicate co-expression of Kv1.3 and GFAP in the same cell. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-25940-5), licensed under a CC-BY licence.

Glial cells density is differently modulated during AD progression.a Retinal slices were immunolabeled with anti-GFAP antibody (green) and Hoechst for nuclei visualization (blue) at different ages of 3xTg-AD and non-Tg mice and density of GFAP signal was quantified as shown in b (**p < 0.01 pre vs early; n = 16 fields/four slices for each condition; two-way ANOVA, Holm-Sidak; ##p < 0.01 for comparison with age-matched non-Tg mice, two-way ANOVA, Holm-Sidak). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29880901), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-05198

GFAP Antibody

(1 Review)

(40 Publications)

0.1 ml

NBP1-05198

Immunohistochemistry Free-Floating: GFAP Antibody [NBP1-05198] - Staining of a section of mouse hippocampus stained with chicken pAb to GFAP, NBP1-05198, dilution 1:5000 in green and costained with rabbit pAb to FOX3/NeuN, dilution 1:5000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains a network of astroglial cells while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons.

Western Blot: GFAP Antibody [NBP1-05198] - Analysis of whole brain lysates using chicken pAb to GFAP, NBP1-05198, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain. The strong band at about 50 kDa corresponds to the GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.

Reactivity:

Hu, Mu, Rt, Po, Bv, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Eq

Applications:

, , ,

Applications: , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-05197

GFAP Antibody (5C10)

(1 Review)

(25 Publications)

0.1 ml

NBP1-05197

Immunohistochemistry Free-Floating: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse GFAP mAb, dilution 1:1,000 (Green), costained with rabbit neurofilament NF-L pAb, dilution 1:2,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with antibodies. The GFAP antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.

Simple Western: GFAP Antibody (5C10) [NBP1-05197] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry: GFAP Antibody (5C10) [NBP1-05197] - The immunofluorescence of TLR7 recognized by Alexa 488, green (NBP2-24906). GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license.

Immunohistochemistry: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse mAb to GFAP, NBP1-05197, dilution 1:1,000, in green, costained with rabbit pAb to neurofilament NF-L, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-05197 antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.

Western Blot: GFAP Antibody (5C10) [NBP1-05197] - Analysis of whole tissue lysates using mouse mAb to GFAP, NBP1-05197, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 50kDa corresponds to the GFAP protein.

Reactivity:

Hu, Mu, Rt, Po, Bv, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Eq

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-05197AF350
Alexa Fluor 405	NBP1-05197AF405
Alexa Fluor 488	NBP1-05197AF488
Alexa Fluor 532	NBP1-05197AF532
Alexa Fluor 594	NBP1-05197AF594
Alexa Fluor 647	NBP1-05197AF647
Alexa Fluor 700	NBP1-05197AF700
Alexa Fluor 750	NBP1-05197AF750
Biotin	NBP1-05197B
DyLight 350	NBP1-05197UV
DyLight 405	NBP1-05197V
DyLight 488	NBP1-05197G
DyLight 550	NBP1-05197R
DyLight 594	NBP1-05197DL594
DyLight 650	NBP1-05197C
DyLight 680	NBP1-05197FR
DyLight 755	NBP1-05197IR
FITC	NBP1-05197F
HRP	NBP1-05197H
Janelia Fluor 549	NBP1-05197JF549
Janelia Fluor 646	NBP1-05197JF646
mFluor Violet 450 SE	NBP1-05197MFV450
mFluor Violet 500 SE	NBP1-05197MFV500
mFluor Violet 610 SE	NBP1-05197MFV610

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NBP2-29415

GFAP Antibody (GA-5)

(2 Reviews)

(19 Publications)

0.1 mg

NBP2-29415

Immunocytochemistry/Immunofluorescence: GFAP Antibody (GA-5) [NBP2-29415] - Cultured Rat Hippocampal Neurons. ICC/IF image submitted by a verified customer review.

Western Blot: GFAP Antibody (GA-5) [NBP2-29415] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (GA-5).

Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) [NBP2-29415] - Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Antibody (GA-5).

Flow Cytometry: GFAP Antibody (GA-5) [NBP2-29415] - Flow Cytometric Analysis of T98G cells using GFAP Antibody (GA-5) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Western Blot: GFAP Antibody (GA-5) [NBP2-29415] - Analysis of GFAP in human brain lysate using GFAP (GA5) antibody at 1 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) [NBP2-29415] - Formalin-paraffin human brain stained with GFAP Ab (GA-5). Note cytoplasmic staining.

Flow Cytometry: GFAP Antibody (GA-5) [NBP2-29415] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and mononuclear cells were isolated from the CNS at day 10 (onset of symptoms). Cells were stained for GFAP, Neun, CX3CL1, CXCL12, CCL2, CD45 and CD11b, plus for viability to exclude dead cells. GFAP staining is shown for viable cells. Flow cytometry image submitted by a verified customer review.

Simple Western: GFAP Antibody (GA-5) [NBP2-29415] - Simple Western lane view shows a specific band for GFAP in 0.2 mg/mL of human brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: GFAP Antibody (GA-5) [NBP2-29415] - Electropherogram image of the corresponding Simple Western lane. GFAP antibody was used at 10 ug/mL dilution of human brain lysate.

Immunohistochemistry-Paraffin: Mouse Monoclonal GFAP Antibody (GA-5) [NBP2-29415] - Analysis of GFAP on adult human brain tissue. Antigen retrieval in a basic buffer x200 (1ug/mL). Image from a verified customer review.

Advancing super-resolution microscopy for astroglial research. (D) GFAP stained with monoclonal antibody (Novus, GA5, secondary Alexa Fluor 488 donkey anti-mouse antibody, Life Technologies) shown at lower (D1) and higher (D2, fragment indicated in D1) magnification. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25782611), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Rb

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

NBO-107A

Alt Catalog# NBO-107A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33184AF350
Alexa Fluor 405	NBP2-33184AF405
Alexa Fluor 488	NBP2-33184AF488
Alexa Fluor 532	NBP2-33184AF532
Alexa Fluor 594	NBP2-33184AF594
Alexa Fluor 647	NBP2-33184AF647
Alexa Fluor 700	NBP2-33184AF700
Alexa Fluor 750	NBP2-33184AF750
Allophycocyanin	NBP2-33184APC
Biotin	NBP2-33184B
DyLight 350	NBP2-33184UV
DyLight 405	NBP2-33184V
DyLight 488	NBP2-33184G
DyLight 550	NBP2-33184R
DyLight 594	NBP2-33184DL594
DyLight 650	NBP2-33184C
DyLight 680	NBP2-33184FR
DyLight 755	NBP2-33184IR
FITC	NBP2-33184F
HRP	NBP2-33184H
Janelia Fluor 549	NBP2-33184JF549
Janelia Fluor 646	NBP2-33184JF646
PE	NBP2-33184PE
PerCP	NBP2-33184PCP
mFluor Violet 450 SE	NBP2-33184MFV450
mFluor Violet 500 SE	NBP2-33184MFV500
mFluor Violet 610 SE	NBP2-33184MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33184
Azide and BSA Free	NBP2-33184-1mg

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AF2594

GFAP Antibody [Unconjugated]

(1 Review)

(14 Publications)

100 μg

AF2594

Western blot shows lysates of rat cortical stem cells, rat brain tissue, human brain (cortex) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

beta ‑III Tubulin was detected in rat cortical neurons using 5 µg/mL Mouse Anti-neuron-specific Mouse beta ‑III Tubulin Monoclonal (clone TuJ‑1) Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the NorthernLights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

GFAP was detected in immersion fixed rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

GFAP was detected in immersion fixed 7 days differentiated rat cortical stem cells using Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51 kDa (as indicated) using 0.1 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 55 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2594

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2594-SP

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NB100-53809

GFAP Antibody

(17 Publications)

0.1 mg

NB100-53809

Western Blot: GFAP Antibody [NB100-53809] - (0.005ug/ml) staining of Human Cerebellum (A) and (0.01ug/ml) Cerebral Cortex (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

Western Blot: GFAP Antibody [NB100-53809] - (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Reactivity:

Hu, Mu, Rt, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Ca

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-30184

Alt Catalog# IMG-30184

Conjugate:

Unconjugated

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MAB2594

GFAP Antibody (273807) [Unconjugated]

(2 Reviews)

(8 Publications)

100 μg

MAB2594

Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GFAP was detected in immersion fixed differentiated rat cortical stem cells using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Simple Western lane view shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51-52 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

GFAP was detected in immersion fixed U937 human histiocytic lymphoma cell line but is not detected in GFAP knockout (KO) U937 Human cell line using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2594-SP

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NBP2-89203

GFAP Antibody (012)

(16 Publications)

100 ul

NBP2-89203

Western Blot: GFAP Antibody (012) [NBP2-89203] - Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 45 kDa.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89203AF350
Alexa Fluor 405	NBP2-89203AF405
Alexa Fluor 488	NBP2-89203AF488
Alexa Fluor 532	NBP2-89203AF532
Alexa Fluor 594	NBP2-89203AF594
Alexa Fluor 647	NBP2-89203AF647
Alexa Fluor 700	NBP2-89203AF700
Alexa Fluor 750	NBP2-89203AF750
Allophycocyanin	NBP2-89203APC
Biotin	NBP2-89203B
DyLight 350	NBP2-89203UV
DyLight 405	NBP2-89203V
DyLight 488	NBP2-89203G
DyLight 550	NBP2-89203R
DyLight 594	NBP2-89203DL594
DyLight 650	NBP2-89203C
DyLight 680	NBP2-89203FR
DyLight 755	NBP2-89203IR
FITC	NBP2-89203F
HRP	NBP2-89203H
Janelia Fluor 549	NBP2-89203JF549
Janelia Fluor 646	NBP2-89203JF646
PE	NBP2-89203PE
PerCP	NBP2-89203PCP
mFluor Violet 450 SE	NBP2-89203MFV450
mFluor Violet 500 SE	NBP2-89203MFV500
mFluor Violet 610 SE	NBP2-89203MFV610

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NBP2-34353

GFAP Antibody (SPM248)

(1 Review)

(2 Publications)

0.1 mg

NBP2-34353

Western Blot: GFAP Antibody (SPM248) [NBP2-34353] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (SPM248).

Immunohistochemistry-Paraffin: GFAP Antibody (SPM248) [NBP2-34353] - Formalin-fixed, paraffin-embedded human schwannoma stained with GFAP monoclonal antibody (SPM248).

Flow Cytometry: GFAP Antibody (SPM248) [NBP2-34353] - Flow Cytometric Analysis of T98G cells using GFAP Antibody (SPM248) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Rb

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-34401AF350
Alexa Fluor 405	NBP2-34401AF405
Alexa Fluor 488	NBP2-34401AF488
Alexa Fluor 532	NBP2-34401AF532
Alexa Fluor 594	NBP2-34401AF594
Alexa Fluor 647	NBP2-34401AF647
Alexa Fluor 700	NBP2-34401AF700
Alexa Fluor 750	NBP2-34401AF750
Allophycocyanin	NBP2-34401APC
Biotin	NBP2-34401B
DyLight 350	NBP2-34401UV
DyLight 405	NBP2-34401V
DyLight 488	NBP2-34401G
DyLight 550	NBP2-34401R
DyLight 594	NBP2-34401DL594
DyLight 650	NBP2-34401C
DyLight 680	NBP2-34401FR
DyLight 755	NBP2-34401IR
FITC	NBP2-34401F
HRP	NBP2-34401H
Janelia Fluor 549	NBP2-34401JF549
Janelia Fluor 646	NBP2-34401JF646
PE	NBP2-34401PE
PerCP	NBP2-34401PCP
mFluor Violet 450 SE	NBP2-34401MFV450
mFluor Violet 500 SE	NBP2-34401MFV500
mFluor Violet 610 SE	NBP2-34401MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-34401-0.1mg

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NB110-93480

GFAP Antibody - Azide and BSA Free

(2 Publications)

0.1 ml

NB110-93480

Western Blot: GFAP Antibody [NB110-93480] - Rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (1:1000, green) and anti-MAP2 (1:1000, red).

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Showing specific labeling of GFAP (red) and vimentin (green) cultured neurons and glia. Cells containing GFAP and vimentin appear yellow.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (1:5000, green), colabeled with anti-MeCP2 (1:500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (1:5000,green), colabeled with anti-calbindin (1:2,000, red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (1:1000, green) and anti-MAP2 (1:1000, red).

Reactivity:

Hu, Mu, Rt, Bv, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Eq

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-34365

GFAP Antibody (SPM507)

(1 Review)

(1 Publication)

0.1 mg

NBP2-34365

Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).

Immunohistochemistry-Paraffin: GFAP Antibody (SPM507) [NBP2-34365] - Formalin-paraffin human brain stained with GFAP Ab (SPM507). Note cytoplasmic staining.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Rb

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-34413AF350
Alexa Fluor 405	NBP2-34413AF405
Alexa Fluor 488	NBP2-34413AF488
Alexa Fluor 532	NBP2-34413AF532
Alexa Fluor 594	NBP2-34413AF594
Alexa Fluor 647	NBP2-34413AF647
Alexa Fluor 700	NBP2-34413AF700
Alexa Fluor 750	NBP2-34413AF750
Allophycocyanin	NBP2-34413APC
Biotin	NBP2-34413B
DyLight 350	NBP2-34413UV
DyLight 405	NBP2-34413V
DyLight 488	NBP2-34413G
DyLight 550	NBP2-34413R
DyLight 594	NBP2-34413DL594
DyLight 650	NBP2-34413C
DyLight 680	NBP2-34413FR
DyLight 755	NBP2-34413IR
FITC	NBP2-34413F
HRP	NBP2-34413H
Janelia Fluor 549	NBP2-34413JF549
Janelia Fluor 646	NBP2-34413JF646
PE	NBP2-34413PE
PerCP	NBP2-34413PCP
mFluor Violet 450 SE	NBP2-34413MFV450
mFluor Violet 500 SE	NBP2-34413MFV500
mFluor Violet 610 SE	NBP2-34413MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-34413-0.1mg

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NBP2-76477

GFAP Antibody (CL2713)

100 ul

NBP2-76477

Knockdown Validated: GFAP Antibody (CL2713) [NBP2-76477] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GFAP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of human cerebral cortex shows strong cytoplasmic positivity in astrocytes.

Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of rat brain shows strong positivity in astrocytes in the cerebellum.

Western Blot: GFAP Antibody (CL2713) [NBP2-76477] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue.

Western Blot: GFAP Antibody (CL2713) [NBP2-76477] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue.

Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of mouse brain shows strong positivity in astrocytes in the cerebellum.

Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of mouse brain shows strong positivity in astrocytes in the brainstem.

Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of rat brain shows strong positivity in astrocytes in the hippocampus.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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