| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1965SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1974-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36571SS | |||
| Azide and BSA Free | NBP2-80609 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-518-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36570SS | |||
| Azide and BSA Free | NBP2-80608 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36571SS | |||
| BSA Free | NBP2-36571 | |||
![Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NB100-1965-img0011.jpg "Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.")
![Western Blot: Calnexin Antibody [NB100-1965] - Subcellular localization of TM6SF2.Immunoaffinity isolation of ER and Golgi complex from mouse liver. ER and Golgi fractions were prepared from mouse liver microsomes by immunoaffinity chromatography as described under Experimental Procedures. Microsome membranes were dissolved in RIPA buffer, and equal volumes were separated on 10% SDS-PAGE and immunoblotting as described under Experimental Procedures. BiP, binding immunoglobulin protein; Gos28, Golgi SNAP receptor complex member 1; *, nonspecific band. Image collected and cropped by Citeab from the following publication (Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NB100-1965-img0015.jpg)
![Immunohistochemistry: Calnexin Antibody [NB100-1965] - Calnexin immunostaining in the anterior (AI) and mid (MI) intestine of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5-T2 (D.I.O. zebrafish followed by 3,5-T2), D.I.O. with 3,5-T2 (D.I.O. zebrafish treated with 3,5-T2). (A) AI of ctrl zebrafish (B) AI of D.I.O. (C) AI of D.I.O. flw 3,5-T2. (D) AI of D.I.O. with 3,5-T2. The arrows indicate calnexin immunoexpression in the enteroendocrine and goblet cells. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2076-2615/10/7/1131/htm#) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-NB100-1965-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1965) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1965-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1965) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1965-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Calnexin Antibody (NB100-1965) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1965-img0020.jpg)
![Western Blot: Calnexin Antibody - BSA Free [NB100-1965] - Whole cell lysate or exosome from MDA-MD-231 cells was loaded with 20 ug/lane. 10% SDS-PAGE. Calnexin Antibody (NB100-1965) was used for primary antibody: 1:3000, 4C, overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/Calnexin-Antibody---BSA-Free-Western-Blot-NB100-1965-img0024.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on rat FR cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1965-img0023.jpg)
![Western Blot: Calnexin Antibody [NB100-1965] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NB100-1965-img0008.jpg)
![Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-NB100-1965-img0010.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on Daudi cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1965-img0021.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on NIH3T3 cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1965-img0022.jpg)
![Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Simple-Western-NB100-1965-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1974) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1974-img0012.jpg "Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1974) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1974) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1974-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1974) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1974-img0014.jpg)
![Western Blot: Calnexin Antibody [NB100-1974] - Identification of plasma exosomes and differentially expressed exosomal miRNAs. Confirmation of the exosomes markers with Western blotting indicated the presence of CD63 but the absence of calnexin in exosomes. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fonc.2019.00459/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NB100-1974-img0010.jpg)
![Immunohistochemistry: Calnexin Antibody [NB100-1974] - Analysis of Calnexin in mouse bladder using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-NB100-1974-img0005.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on rat FR cells with Calnexin Antibody NB100-1974 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1974-img0017.jpg)
![Western Blot: Calnexin Antibody [NB100-1974] - Analysis of Calnexin in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NB100-1974-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - Zebrafish Vwf forms pseudo-Weibel-Palade bodies (pseudo-WPBs) in mammalian cell culture. pzVwf/Myc-HIS (zebrafish Vwf) plasmids were transfected into HEK293T cells. Anti-Myc antibody conjugated to Alexa Fluor 488 (green channel) was used for detection and anti-calnexin antibody conjugated to Alexa Fluor 594 (red channel) labeled endoplasmic reticulum (ER). Both constructs demonstrate formation of elongated Myc positive and ER negative structures (absence of yellow signal in the merged panels) characteristic of pseudo-WPBs. Scale bars, 2.5 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2012/214209/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1974-img0011.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1974] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-1974. An intracellular stain was performed on Jurkat cells with Calnexin antibody NB100-1974AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1974-img0008.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on HeLa cells with Calnexin antibody NB100-1974AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1974-img0009.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on Daudi cells with Calnexin Antibody NB100-1974 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1974-img0015.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on NIH3T3 cells with Calnexin Antibody NB100-1974 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1974-img0016.jpg)
![Simple Western: Calnexin Antibody [NB100-1974] - Image shows a specific band for Calnexin in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Simple-Western-NB100-1974-img0006.jpg)
![Western Blot: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone IE2.1C12) at 0.5 ug/ml concentration.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Western-Blot-NBP2-36571-img0008.jpg "Western Blot: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone IE2.1C12) at 0.5 ug/ml concentration.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Calnexin (1E2.1C12) antibody was tested in Hela cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Immunocytochemistry-Immunofluorescence-NBP2-36571-img0015.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cells of Glomeruli developed strong cytoplasmic staining.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Immunohistochemistry-Paraffin-NBP2-36571-img0009.jpg)
![Flow Cytometry: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of Allophycocyanin conjugate of NBP2-36571. An intracellular stain was performed on Jurkat cells with Calnexin antibody (IE2.1C12) NBP2-36571APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Flow-Cytometry-NBP2-36571-img0016.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Immunohistochemistry-Paraffin-NBP2-36571-img0003.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Immunohistochemistry-Paraffin-NBP2-36571-img0012.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Immunohistochemistry-Paraffin-NBP2-36571-img0011.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Immunohistochemistry-Paraffin-NBP2-36571-img0010.jpg)
![Flow (Intracellular): Calnexin Antibody (IE2.1C12) [NBP2-36571] - Intracellular staining of human Calnexin in Flow cytometry using 5.0 ug of antibody per 1 million cells. Isotype control was mouse IgG2b kappa.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Flow-Intracellular-NBP2-36571-img0002.jpg)
![Western Blot: Calnexin Antibody (AF18) [NB300-518] - Analysis of 25 ug of HepG2 lysate.](http://images.novusbio.com/fullsize/Calnexin-Antibody-AF18-Western-Blot-NB300-518-img0015.jpg "Western Blot: Calnexin Antibody (AF18) [NB300-518] - Analysis of 25 ug of HepG2 lysate.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (AF18) [NB300-518] - Staining of calnexin (red) in U87 cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-AF18-Immunocytochemistry-Immunofluorescence-NB300-518-img0012.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (AF18) [NB300-518] - Both normal and cancer biopsies of deparaffinized Human hepatocarcinoma tissues.](http://images.novusbio.com/fullsize/Calnexin-Antibody-AF18-Immunohistochemistry-Paraffin-NB300-518-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (AF18) [NB300-518] - Transfected COS7 cells were blocked in 1% BSA, 5% normal goat serum, and 0.1% Triton X-100 in 1X PBS, and then stained (1:500), followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody (red).](http://images.novusbio.com/fullsize/Calnexin-Antibody-AF18-Immunocytochemistry-Immunofluorescence-NB300-518-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (AF18) [NB300-518] - Staining of Calnexin in A549 Cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-AF18-Immunocytochemistry-Immunofluorescence-NB300-518-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (AF18) [NB300-518] - Staining of HMVEC Cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-AF18-Immunocytochemistry-Immunofluorescence-NB300-518-img0011.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (AF18) [NB300-518] - Normal deparaffinized Human kidney tissue tissues.](http://images.novusbio.com/fullsize/Calnexin-Antibody-AF18-Immunohistochemistry-Paraffin-NB300-518-img0013.jpg)
![Western Blot: Calnexin Antibody [NBP1-97485] - Analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-97485-img0003.jpg "Western Blot: Calnexin Antibody [NBP1-97485] - Analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97485] - Analysis of human spleen tissue stained with Calnexin, pAb at 10ug/ml.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-97485-img0002.jpg)
![Western Blot: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone 1C2.2D11) at 0.5 ug/mL.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Western-Blot-NBP2-36570-img0008.jpg "Western Blot: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone 1C2.2D11) at 0.5 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [NBP2-36570] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Calnexin (1C2.2D11) conjugated to FITC [NBP2-36570F] at 10ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunocytochemistry-Immunofluorescence-NBP2-36570-img0012.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cells of Glomeruli developed strong cytoplasmic staining.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunohistochemistry-Paraffin-NBP2-36570-img0010.jpg)
![Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - An intracellular stain was performed on HeLa cells with Calnexin [1C2.2D11] Antibody NBP2-36570AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Flow-Intracellular-NBP2-36570-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Calnexin (1C2.2D11) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunocytochemistry-Immunofluorescence-NBP2-36570-img0009.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunohistochemistry-Paraffin-NBP2-36570-img0003.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunohistochemistry-Paraffin-NBP2-36570-img0005.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunohistochemistry-Paraffin-NBP2-36570-img0006.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunohistochemistry-Paraffin-NBP2-36570-img0007.jpg)
![Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - Intracellular staining of human Calnexin in Flow cytometry using 5.0 ug of antibody per 1 million cells. Isotype control was mouse IgG2b kappa.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Flow-Intracellular-NBP2-36570-img0002.jpg)
![Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - An intracellular stain was performed on HeLa cells with Calnexin Antibody (1C2.2D11) NBP2-36570F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Flow-Intracellular-NBP2-36570-img0011.jpg)
![Western Blot: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone IE2.1C12) at 0.5 ug/ml concentration. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Western-Blot-NBP2-80609-img0001.jpg "Western Blot: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone IE2.1C12) at 0.5 ug/ml concentration. Image from the standard format of this antibody.")
![Immunocytochemistry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80609-img0003.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cells of Glomeruli developed strong cytoplasmic staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80609-img0007.jpg)
![Flow Cytometry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of Allophycocyanin conjugate of NBP2-36571. An intracellular stain was performed on Jurkat cells with Calnexin antibody (IE2.1C12) NBP2-36571APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80609-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Calnexin (1E2.1C12) antibody was tested in Hela cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80609-img0002.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein. Image from the standar](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80609-img0004.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin. Image from t](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80609-img0005.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining. Image from the standard form](http://images.novusbio.com/fullsize/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80609-img0006.jpg)
![Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Cells cultured without pyruvate were exposed to Akt inhibitor (AI, 5 uM) or rapamycin (1, 10, or 100 nM), and mTOR phosphorylation, total mTOR levels and phosphorylation of p70S6K was assessed, calnexin was used as a protein loading control. The numbers above the blots indicate phospho-S6K levels relative to calnexin. Image collected and cropped by CiteAb from the following publication (frontiersin.org/articles/10.3389/fcell.2017.00054/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1563-Western-Blot-NBP2-43765-img0008.jpg "Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Cells cultured without pyruvate were exposed to Akt inhibitor (AI, 5 uM) or rapamycin (1, 10, or 100 nM), and mTOR phosphorylation, total mTOR levels and phosphorylation of p70S6K was assessed, calnexin was used as a protein loading control. The numbers above the blots indicate phospho-S6K levels relative to calnexin. Image collected and cropped by CiteAb from the following publication (frontiersin.org/articles/10.3389/fcell.2017.00054/full), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1563) [NBP2-43765] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Calnexin stained by Calnexin antibody [1563] diluted at 1:500. Red: Calnexin, stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1563-Immunocytochemistry-Immunofluorescence-NBP2-43765-img0007.jpg)
![Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1563-Western-Blot-NBP2-43765-img0001.jpg)
![Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:500](http://images.novusbio.com/fullsize/Calnexin-Antibody-1563-Western-Blot-NBP2-43765-img0002.jpg)
![Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 50 ug mouse liver lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000](http://images.novusbio.com/fullsize/Calnexin-Antibody-1563-Western-Blot-NBP2-43765-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [1563] (NBP2-43765).](http://images.novusbio.com/fullsize/Calnexin-Antibody-1563-Western-Blot-NBP2-43765-img0006.jpg)
![Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-85519-img0018.jpg "Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NBP1-85519] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85519-img0006.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0017.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex, placenta, testis and tonsil using Anti-Calnexin antibody NBP1-85519 (A) shows similar protein distribution across tissues to independent antibody NBP1-85520 (B).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0014.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0015.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0016.jpg)
![Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.](http://images.novusbio.com/fullsize/Calnexin-Antibody-3H4A7-Western-Blot-NBP1-47546-img0002.jpg "Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (3H4A7) [NBP1-47546] - Confocal immunofluorescence analysis of Hela cells using anti-Calnexin mAb (green).](http://images.novusbio.com/fullsize/Calnexin-Antibody-3H4A7-Immunocytochemistry-Immunofluorescence-NBP1-47546-img0003.jpg)
![Western Blot: Calnexin Antibody [NBP1-37774] - Staining of CaCo-2 (A) with antibody at 0.1 ug/mL and NIH3T3 (B) cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-37774-img0002.jpg "Western Blot: Calnexin Antibody [NBP1-37774] - Staining of CaCo-2 (A) with antibody at 0.1 ug/mL and NIH3T3 (B) cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NBP1-37774] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-37774-img0003.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-37774] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-37774-img0004.jpg)
![Western Blot: Calnexin Antibody [NBP1-37774] - )Staining of Human Cerebellum (A) and Colorectal cancer (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-37774-img0001.jpg)
![Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-97476-img0002.jpg "Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97476] - Analysis of human placenta tissue stained with Calnexin, pAb at 5ug/ml.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-97476-img0001.jpg)