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930-ADB

Recombinant Human TACE/ADAM17 Protein, CF

(29 Publications)

10 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB9301

TACE/ADAM17 Antibody (111633) [Unconjugated]

(1 Review)

(17 Publications)

100 μg

MAB9301

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Ectodomain Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

<b>Genetic Strategies Validation. </b>TACE/ADAM17 knockout HeLa epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the TACE/ADAM17 knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Effects of the engineered S197P mutation on CD16a shedding in NK cells.NK92 cells transduced with empty vector (vector only), CD16a, or CD16a/S197P were treated without (Unstim.) or with PMA (100ng/ml) for 30 minutes at 37°C (A), with IL-12 and IL-18 (100ng/ml and 400ng/ml, respectively) for 24 hours at 37°C (B), or with Raji cells and rituximab for 60 min at 37°C (C). Cell surface levels of CD16a were determined by flow cytometry. Isotype-matched negative control antibody staining is indicated by a dotted line. (D) Parent NK92 cells and transduced cells expressing CD16a or CD16a/S197P were treated with Raji cells and rituximab in the presence or absence of the ADAM17 inhibitor BMS566394 (5μM) for 60 min at 37°C. Soluble CD16a levels were determined by ELISA. Each treatment condition was repeated 3 times and the data are representative of 3 independent experiments. Bar graphs show mean ± SD. Statistical significance is indicated as ***P<0.001. (E) NK92 cells expressing CD16a or CD16a/S197P were stained with the anti-ADAM17 mAbs M220, 623, 633, or an isotype-matched negative control antibody, as indicated. (F) CD56+CD45+ NK cells derived from mock-transduced iPSCs (left panel) or iPSCs expressing recombinant CD16a or CD16a/S197P (right panels) were incubated with or without K562 target cells for 4 hours at 37°C. For all histogram plots, the x-axis = Log 10 fluorescence, the y-axis = cell number, and the data are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0121788), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Sample Type:

23038

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9301U-100UG
Alexa Fluor 405	FAB9301V-100UG
Alexa Fluor 488	FAB9301G-100UG
Alexa Fluor 532	FAB9301X
Alexa Fluor 594	FAB9301T-100UG
Alexa Fluor 647	FAB9301R-100UG
Alexa Fluor 700	FAB9301N-100UG
Alexa Fluor 750	FAB9301S-100UG
Allophycocyanin	FAB9301A
Allophycocyanin/Cy7	FAB9301APCCY7
Biotin	FAB9301B
DyLight 350	FAB9301D
DyLight 405	FAB9301E
DyLight 488	FAB9301K
DyLight 550	FAB9301L
DyLight 594	FAB9301M
DyLight 650	FAB9301W
DyLight 680	FAB9301Y
DyLight 755	FAB9301Z
HRP	FAB9301H
Janelia Fluor 549	FAB9301I
Janelia Fluor 646	FAB9301J
PE/Atto594	FAB9301PEATT594
PE/Cy5.5	FAB9301PECY55
PE/Cy7	FAB9301PECY7
PerCP	FAB9301C
Phycoerythrin	FAB9301P
mFluor Violet 450 SE	FAB9301MFV450
mFluor Violet 500 SE	FAB9301MFV500
mFluor Violet 610 SE	FAB9301MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB9301P
Carrier Free	MAB9301-SP
Carrier Free	MAB9301-500
Carrier Free	FAB9301V-100UG
Carrier Free	FAB9301U-100UG
Carrier Free	FAB9301T-100UG
Carrier Free	FAB9301S-100UG
Carrier Free	FAB9301R-100UG
Carrier Free	FAB9301N-100UG
Carrier Free	FAB9301G-100UG

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