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33 Results for "novus"
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AF2229

Nectin-2/CD112 Antibody [Unconjugated]

(10 Publications)
100 μg
AF2229
Western blot shows lysates of 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Nectin‑2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Nectin‑2/CD112 at approximately 60-75 kDa (as indicated). Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line are shown as negative controls. GAPDH (Catalog # AF5718)is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, IHC, ICC/IF
Applications: WB, Simple Western, IHC, ICC/IF
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF2229-SP
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MAB3869

Nectin-2/CD112 Antibody (829038)

(7 Publications)
100 μg
MAB3869
C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Nectin‑2/CD112 Monoclonal Antibody (Catalog # MAB3869, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Mu
Species Glossary
Reactivity: Mu
Flow, CyTOF-ready
Applications: Flow, CyTOF-ready
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB3869U-100UG
Alexa Fluor 405 FAB3869V-100UG
Alexa Fluor 488 FAB3869G
Alexa Fluor 532 FAB3869X
Alexa Fluor 594 FAB3869T-100UG
Alexa Fluor 647 FAB3869R-100UG
Alexa Fluor 700 FAB3869N-100UG
Alexa Fluor 750 FAB3869S-100UG
Allophycocyanin FAB3869A
Allophycocyanin/Cy7 FAB3869APCCY7
Biotin FAB3869B
DyLight 350 FAB3869D
DyLight 405 FAB3869E
DyLight 488 FAB3869K
DyLight 550 FAB3869L
DyLight 594 FAB3869M
DyLight 650 FAB3869W
DyLight 680 FAB3869Y
DyLight 755 FAB3869Z
FITC FAB3869F
HRP FAB3869H
Janelia Fluor 549 FAB3869I
Janelia Fluor 646 FAB3869J
PE/Atto594 FAB3869PEATT594
PE/Cy5.5 FAB3869PECY55
PE/Cy7 FAB3869PECY7
PerCP FAB3869C
Phycoerythrin FAB3869P
mFluor Violet 450 SE FAB3869MFV450
mFluor Violet 500 SE FAB3869MFV500
mFluor Violet 610 SE FAB3869MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free FAB3869P
Carrier Free FAB3869A
Carrier Free FAB3869G
Carrier Free MAB3869-SP
Carrier Free FAB3869V-100UG
Carrier Free FAB3869U-100UG
Carrier Free FAB3869T-100UG
Carrier Free FAB3869S-100UG
Carrier Free FAB3869R-100UG
Carrier Free FAB3869N-100UG
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MAB2229

Nectin-2/CD112 Antibody (610603) [Unconjugated]

100 μg
MAB2229
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Nectin‑2/CD112 Monoclonal Antibody (Catalog # MAB2229, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-mouse secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Hu
Species Glossary
Reactivity: Hu
Flow, ICC/IF
Applications: Flow, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB2229U-100UG
Alexa Fluor 405 FAB2229V-100UG
Alexa Fluor 488 FAB2229G
Alexa Fluor 532 FAB2229X
Alexa Fluor 594 FAB2229T-100UG
Alexa Fluor 647 FAB2229R-100UG
Alexa Fluor 700 FAB2229N
Alexa Fluor 750 FAB2229S-100UG
Allophycocyanin FAB2229A
Allophycocyanin/Cy7 FAB2229APCCY7
Biotin FAB2229B
DyLight 350 FAB2229D
DyLight 405 FAB2229E
DyLight 488 FAB2229K
DyLight 550 FAB2229L
DyLight 594 FAB2229M
DyLight 650 FAB2229W
DyLight 680 FAB2229Y
DyLight 755 FAB2229Z
FITC FAB2229F
HRP FAB2229H
Janelia Fluor 549 FAB2229I
Janelia Fluor 646 FAB2229J
PE/Atto594 FAB2229PEATT594
PE/Cy5.5 FAB2229PECY55
PE/Cy7 FAB2229PECY7
PerCP FAB2229C
Phycoerythrin FAB2229P
mFluor Violet 450 SE FAB2229MFV450
mFluor Violet 500 SE FAB2229MFV500
mFluor Violet 610 SE FAB2229MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free FAB2229P
Carrier Free FAB2229A
Carrier Free FAB2229G
Carrier Free FAB2229N
Carrier Free MAB2229-SP
Carrier Free FAB2229U-100UG
Carrier Free FAB2229S-100UG
Carrier Free FAB2229T-100UG
Carrier Free FAB2229R-100UG
Carrier Free FAB2229V-100UG
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NBP1-91211

Nectin-2/CD112 Antibody

(1 Publication)
0.1 ml
NBP1-91211
Immunohistochemistry-Paraffin: Nectin-2/CD112 Antibody [NBP1-91211] - Staining in human placenta and cerebral cortex tissues . Corresponding NECTIN2 RNA-seq data are presented for the same tissues.
Hu
Species Glossary
Reactivity: Hu
IHC
Applications: IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
         
Validated by:          
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AF3869

Nectin-2/CD112 Antibody

100 μg
AF3869
Western blot shows lysates of C2C12 mouse myoblast cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Nectin‑2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3869) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nectin‑2/CD112 at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Sheep Polyclonal
Host: Sheep Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF3869-SP
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10617-N2

Recombinant Cynomolgus Monkey Nectin-2/CD112 His Protein, CF

50 μg
10617-N2
When Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera (9276-DN) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey Nectin-2/CD112 His-tag (Catalog # 10617-N2) binds with an ED50 of 0.4-2.4 μg/mL.
Pm-Cm
Species Glossary
Reactivity: Pm-Cm
Bioactivity
Applications: Bioactivity
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10485-N2

Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein, CF

100 μg
10485-N2
When Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Chimera (10485-N2) is immobilized at 1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera (9276-DN) binds with an ED50 of 0.1-0.6 μg/mL.
Pm-Cm
Species Glossary
Reactivity: Pm-Cm
Bioactivity
Applications: Bioactivity
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AVI9317

Recombinant Human Nectin-2/CD112 Fc Avi-tag Protein, CF

50 μg
AVI9317
When Recombinant Human DNAM-1 Fc Chimera (666-DN) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Nectin-2/CD112 Fc Chimera Avi-tag (Catalog # AVI9317) binds with an ED50 of 8.00 - 48.0 ng/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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AVI2229

Recombinant Human Nectin-2/CD112 His-tag Avi-tag Protein, CF

50 μg
AVI2229
When Recombinant Human DNAM-1 Fc Chimera (666-DN) is immobilized at 2.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Nectin-2/CD112 His-tag Avi-tag Protein (Catalog # AVI2229) binds with an ED50 of 0.600‑3.60 µg/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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MAB22292

Nectin-2/CD112 Antibody (610611) [Unconjugated]

100 μg
MAB22292
Nectin‑2/CD112 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human Nectin‑2/CD112 Monoclonal Antibody (Catalog # MAB22292) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Hu
Species Glossary
Reactivity: Hu
B/N, ICC/IF
Applications: B/N, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB22292-SP
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2229-N2

Recombinant Human Nectin-2/CD112 Protein

(1 Publication)
50 μg
Hu
Species Glossary
Reactivity: Hu
Binding Activity
Applications: Binding Activity
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free 2229-N2-050/CF
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9317-N2

Recombinant Human Nectin-2/CD112 Fc Chimera Protein, CF

50 μg
9317-N2
When Recombinant Human Nectin‑2/CD112 Fc Chimera(Catalog # 9317-N2) is coated at 0.5 μg/mL, Recombinant HumanDNAM-1 Fc Chimera (Catalog # 666-DN) binds with an ED50 of0.04-0.2 μg/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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