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32 Results for "novus"

NB600-308

GFP Antibody

(18 Reviews)

(262 Publications)

0.1 mg

NB600-308

Analysis of GFP in transgenic mouse pancreas in OCT. The blue region in the center is an islet which does not express GFP and is negative. Image from verified customer review.

Analysis of GFP tagged PKA subunits over-expressed in HEK293T cells using GFP antibody. Image from verified customer review.

Staining of GFP+ hepatocytes (brown) transplanted into the liver of a recipient animal using anti-GFP antibody. Cyclin D1 double staining in blue. Image from verified customer review.

Total cell lysates (20ug) with protein of interest tagged with GFP was loaded. Blotted with GFP antibody [HRP] NB600-308H, without any secondary antibody. Image from verified customer review.

Germaria from wt or chico1/KG mutant ovaries endogenously expressing Myc-GFP stained with anti-GFP, anti-1B1, and DAPI. Scale bars, 10 um. (F) Quantification of relative Myc-GFP intensity in germarium region 2B from wt or chico1/KG mutant ovaries. n = 10 germaria for each genotype. Error bars represent SEM. *p 0.05), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs d. MM – molecular weight marker, C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis Image collected and cropped by CiteAb from the following open publication (//www.rbej.com/content/13/1/110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Protein expression and nuclear foci formation in breast cancer cell lines. (a) Cellular protein levels. 53BP1 and Vimentin band intensities were normalized to loading controls. Values for T47D cell extracts, loaded as a reference on each blot, were set to 1. Mean relative Western blot signals per cell line were calculated from two independent experiments. Box plots in the upper panels show mean values (cross), the median (line), and 95% CI (whiskers) for untreated and for 7d Eribulin-treated non-TNBC (N=4) and TNBC cell lines (N=7). The Kruskal–Wallis test did not reveal significant differences in the case of 53BP1. Representative immunoblots are shown in the bottom panels. Vimentin staining was detected in all TNBC but was negative in all non-TNBC cell lines in which BT20 served as a reference. Actin normalized protein levels are indicated above the 53BP1 and Vimentin signals. (b) Focal accumulation of 53BP1 in the nucleus of MBC cell lines. 53BP1 foci were detected by immunofluorescence microscopy in MBC cell lines. The graph presents quantitative data in box plots as in A; ~300–900 nuclei per sample were scored in two independent experiments each, and mean values were calculated for each cell line. *P<0.05, Kruskal–Wallis test, Mann–Whitney U test. Representative immunofluorescence images are displayed in Supplementary Figure S3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32283863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-308AF350
Alexa Fluor 405	NB600-308AF405
Alexa Fluor 488	NB600-308AF488
Alexa Fluor 532	NB600-308AF532
Alexa Fluor 594	NB600-308AF594
Alexa Fluor 647	NB600-308AF647
Alexa Fluor 700	NB600-308AF700
Alexa Fluor 750	NB600-308AF750
Allophycocyanin	NB600-308APC
Allophycocyanin/Cy7	NB600-308APCCY7
Biotin	NB600-308B
DyLight 350	NB600-308UV
DyLight 405	NB600-308V
DyLight 488	NB600-308G
DyLight 550	NB600-308R
DyLight 594	NB600-308DL594
DyLight 650	NB600-308C
DyLight 680	NB600-308FR
DyLight 755	NB600-308IR
FITC	NB600-308F
HRP	NB600-308H
Janelia Fluor 549	NB600-308JF549
Janelia Fluor 646	NB600-308JF646
PE	NB600-308PE
PE/Atto594	NB600-308PEATT594
PE/Cy5.5	NB600-308PECY55
PE/Cy7	NB600-308PECY7
PerCP	NB600-308PCP
mFluor Violet 450 SE	NB600-308MFV450
mFluor Violet 500 SE	NB600-308MFV500
mFluor Violet 610 SE	NB600-308MFV610

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NB100-1770

GFP Antibody

(7 Reviews)

(122 Publications)

0.1 mg

NB100-1770

Analysis using the Biotin conjugate of NB100-1770. Staining of transgenic mouse pancreas, expressing GFP in beta cells. Image courtesy of product review by Dr. Yves Heremans of Vrije Universiteit Brussel.

Analysis of Biotin conjugate of NB100-1770. Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-GFP antibody at a 1:1000 for 1 h at RT.

Analysis using the FITC conjugate of NB100-1770. Lane 1: GFP (50 ug). Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Block for 30 minutes.

HEK293 cells stable transfected with integrin alpha8-GFP, were immunostained for GFP (green) and counterstained with phalloidin (red) and DAPI (BLUE). ICC/IF image submitted by a verified customer review.

Analysis using the Alkaline Phosphatase conjugate of NB100-1770. Lane 1: GFP (50 ng). Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Predicted/Observed size: 28 kDa for GFP.

9 Poly-GA co-immuno - precipitation with pATM. Aggregated poly-GA selectively co-immunoprecipitates with pATM. Image collected and cropped by CiteAb from the following publication (www.link.springer.com/article/10.1007%2Fs00401-019-02082-0) licensed under a CC-BY license.

E5.5 Hex-GFP transgenic mouse embryo tissue. Primary antibody: Goat anti-GFP at 1:500 dilution. Secondary antibody: Fluorochrome conjugated Anti-goat IgG antibody at 1:10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.

IF analysis of GFP in mouse brain. Image courtesy of product review by Tatyana Pivneva.

Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS o/n, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP was used at 1:500 dilution in free floating immunohistochemistry to detect GFP. Secondary antibody: Fluorochrome conjugated Anti-goat IgG antibody at 1:500 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

Analysis of DyLight 488 conjugate of NB100-1770. Human breast carcinoma tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-Histone and Anti-Tubulin antibody at 10 ug/mL for 1 h at RT. Secondary antibody: DyLight 488.

GFP-positive transplanted NPCs have morphological features of hippocampal pyramidal neurons at day 90 after grafting. IHC image submitted by a verified customer review.

Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary antibody: IRDye(R) 800 conjugated Donkey-a-Goat IgG [H&L] MX7 () secondary antibody at 1:2,500 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 33 kDa for GFP. Other band(s): none.

Western Blot of GFP Antibody. Lane 1: Opal Prestained Molecular Weight Marker

Multi-lysate Western Blot of GFP Antibody. Marker: Opal Pre-stained ladder

hnRNPA3 knockout enhances DNA damage in DPR expressing HeLa cells. a Immunocytochemical detection of gammaH2AX foci in GFP-tagged DPR-expressing HeLa WT and A3KO HeLa cells. b Fold change of gammaH2AX foci in HeLa WT and A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 47-127 cells from 2 biological replicates. c Immunocytochemical detection of pATM foci in GFP-tagged DPR-expressing HeLa WT and A3KO HeLa cells. d Fold change of pATM foci in HeLa WT and A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 71-263 cells from 2 biological replicates. GFP EGFP transfected, GA EGFP-tagged poly-GA 175 repeats transfected, GR EGFP-tagged poly-GR 177 repeats transfected, PR: EGFP-tagged poly-PR 176 repeats transfected. All graphs are shown as mean +/- SEM. *p < 0.05, **p < 0.01; one-way ANOVA and Tukey's post-hoc test. Scale bar 10 um Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31642962), licensed under a CC-BY licence.

HIF1 alpha and PFKFB3 are protective against hIAPP mediated beta -cell death. a Representative immunoblotting (left) and quantification (right) of the whole cell extract (WCE) from asynchronous INS 832/13 cells treated with either HIF1 alpha inhibitor KC7F2 (10 μM) or PFKFB3 siRNA (75 nM) for 56 h versus control. Cells were transduced with hIAPP adenovirus (75 MOI) or LacZ adenovirus as control (75 MOI) for last 32 h of culture. DMSO or CTRL siRNA, respectively were added to LacZ or hIAPP to equal DMSO or PFKFB3 siRNA used in experimental groups. GAPDH was used as a loading control. Data are presented as mean ± SEM, n = 4, *p < 0.05, **p < 0.01. b Quantification of DNA content distribution after FACS analysis of INS 832/13 cells treated as described in (a). Data are the mean ± SEM, n = 4, *p < 0.05, **p < 0.01. c The frequency of TUNEL positive INS 832/13 cells treated as described in (a). Data are the mean ± SEM, n = 4 in (a) and (b), and n = 3 independent experiments in (c). Statistical significance was analyzed by one-way ANOVA test with Tukey’s post-test (*p < 0.05, **p < 0.01). See Supplementary Fig. 10 for additional supportive data Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31213603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, and (F) Cxcl5 in normal control, IgG Ab, and anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , , , , , ,

Applications: , , , , , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alkaline Phosphatase	NB600-1502
Biotin	NB100-1678
DyLight 488	NBP1-69969
FITC	NB100-1771
FITC	NB100-1771SS
HRP	NB600-313
Rhodamine	NBP1-97327-500ug
Texas Red	NB120-6660

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NB100-1614

GFP Antibody

(6 Reviews)

(73 Publications)

0.1 ml

NB100-1614

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - Staining of cultured hippocampal neuron from rat. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - HeLa cells transiently expressing GFP were visualized using GFP antibody (green). Nuclei were counterstained with DAPI. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - IF analysis of GFP in mouse trigeminal ganglia, cornea. Image courtesy of product review by Jessica Newsom.

Immunohistochemistry: GFP Antibody [NB100-1614] - 5dpf Tg(brn3c:GFP) larvae were incubated with vehicle alone (DMSO, A), 400uM of cisplatin (CP, B), pre-treated with Qx for 2 hours and then incubated with CP for 6 hours (Qx, CP, C,E,G) or pre-treated with Qx for 2 hours and then co-treated with Qx and CP for 6 more hours (Qx+CP, D,F,H). Animals were fixed and immunostained for GFP (green) and otoferlin (red). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-33520-w), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - IF analysis of GFP in rat cortical culture (2 weeks old). ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - LNCaP human prostate cancer cell line imaged after transfection with GFP expressing plasmid. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (RED).

Immunohistochemistry-Paraffin: GFP Antibody [NB100-1614] - Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.

Immunohistochemistry-Paraffin: GFP Antibody [NB100-1614] - Embyonic mouse brain section. GFP in green, DAPI in grey. IHC-P image submitted by a verified customer review.

Immunohistochemistry: GFP Antibody [NB100-1614] - (J) control. (K) Neo 200uM 30min. (L) GM 50uM 1 hour. (M) CP: 400uM 2 hours. (N) Qx 300uM 8 hours. (O) Qx 300uM 8 hours + Neo 200uM 30min. (P) Qx 300uM 8 hours + GM 50uM 1 hour. (Q) Qx 300uM 8 hours + CP 400uM 2 hours. Asterisks denote neuromast supporting cells positive for BrdU. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-33520-w), licensed under a CC-BY license.

Comparison between GFP-immunoreactivity using NB100-1614 (left panel in red) and autofluorescence (right panel in green). In this case, the cortical neuron in this unfixed thick section was first photographed for GFP autofluorescence (left), and then the section was fixed (4% paraformaldehyde) and immunostained for GFP-immunoreactivity (1:1000 dilution) using Texas Red-goat anti-chicken IgY antibodies as a secondary. The same cell (left) was then identified. Far right: Western blot showing specific immunolabeling of the GFP protein.

Immunohistochemistry: Chicken Polyclonal GFP Antibody [NB100-1614] - Chicken Polyclonal GFP Antibody on mouse cancer tissue. DAPI (Red) and Cytopastmic GFP stain (Green). Primary antibody dilution: 1:200 in a 200 um slice. Image from a verified customer review.

Intact MOR function in the Oprm1-Cre line. c) Confocal imaging of RNAscope probes targeting eGFP (green) and Oprm1 (purple) mRNAs in addition of DAPI staining (blue) shows colocalization of the two transcripts in dorsal striatum sections of Oprm1Cre/Cre mice (inset). Dashed lines delimit examples of MOR-EGFP/Cre-positive neurons. Magnification, 60x with immersion oil. Scale bar, 5 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32381649), licensed under a CC-BY licence.

Reptin depletion reduces the recruitment on chromatin of BRCA1 and 53BP1 after gamma ray irradiation.HuH7 cells stably expressing a doxycycline-inducible Reptin shRNA were treated with doxycycline (sh Reptin +) or left untreated (sh Reptin-) for 4 days. (A) Representative images of 53BP1 and BRCA1 foci in HuH7 cells detected using immunofluorescence 2h after gamma ray irradiation. (B) The bars represent the mean number of foci per cell from two independent experiments (>200 cells were counted per experiment). Expression levels of BRCA1 and 53BP1 were assessed by Western Blot on whole cell extracts 4 days after doxycycline treatment. A representative picture is shown in (C). The migration positions of molecular weight standards (in kDa) are indicated on the left. (D) Quantification of 3 Western blot experiments. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0123333), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Frozen: Chicken Polyclonal GFP Antibody [NB100-1614] - Image depicting GFP in magenta and CD31 (Catalog # AF3628) in red. Tissue is a fixed frozen section of sciatic nerve obtained from a S100A4 GFP mouse. NB100-1614 was diluted 1 in 10000 and was left on tissue sections overnight at 4 degrees Celsius. Secondary antibody was donkey anti-chicken conjugated to Alexa 647. Image from a verified customer review.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB600-597

GFP Antibody (9F9.F9)

(3 Reviews)

(54 Publications)

0.1 mg

NB600-597

Immunocytochemistry/Immunofluorescence: GFP Antibody (9F9.F9) [NB600-597] - GFP-transducted 293T cells. Image from verified customer review.

Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Lane 1: Molecular Weight Markers. Lane 2: GFP. Load: 10ng per lane. Primary antibody: Ms Anti-GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: Mouse HRP secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 27 kDa for epitope tag GFP. Other band(s): None.

Western Blot: GFP Antibody (9F9.F9) [NB600-597] - All lanes have picogram amount recombinant GFP protein spiked into HeLa cell-derived lysates Lane 1: 64pg 2pg Lane 3: 16pg Lane 4: 8pg Lane 5: 4pg Lane 6: 2pg Lane 7: 1g Lane 8: 0pg. Primary antibody: anti-GFP monoclonal antibody at 1:400 for overnight at 4C. Secondary antibody: HRP-conjugated anti-Mouse IgG was performed at a dilution of 1:20,000 for 1h at 4C. Block: TTBS supplemented with 1% BSA for 1 h at 4C. Predicted/Observed size: 27 kDa for GFP. Other b&(s): none.

Immunohistochemistry: GFP Antibody (9F9.F9) [NB600-597] - Used 1:5000 As referenced in: Lu S-M, Tremblay M-E` , King IL, Qi J, Reynolds HM, et al. (2011) HIV-1 Tat-Induced Microgliosis and Synaptic Damage via Interactions between Peripheral and Central Myeloid Cells. PLoS ONE 6(9): e23915. doi:10.1371/journal.pone.0023915 Image using the Biotin format of this antibody.

Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Expression of GFP in CHO-K1 cells trasfected with GFP. Image from verified customer review.

Immunoprecipitation/Western Blot using GFP Protein. Lane 1: Opal Prestained Molecular Weight Marker

Western blot of GFP Antibody (9F9.F9). Lane 1: Opal Prestained Molecular Weight Marker

Western blot of Mouse Anti-GFP Antibody. Lane 1: Thermo SuperSignal Molecular Weight Marker. Lane 2: GFP protein

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	NB110-40670

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NB600-303

GFP Antibody

(3 Reviews)

(54 Publications)

0.05 ml

NB600-303

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-303] - Analysis of FITC conjugate of NB600-303. Staining of mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP. As seen in: Collins CM, Speck SH (2012) Tracking Murine Gammaherpesvirus 68 Infection of Germinal Center B Cells.

Western Blot: GFP Antibody [NB600-303] - Analysis using the FITC conjugate of NB600-303. Detection of Lane 1: GFP. Load: 50 ug per lane. Primary antibody: none. Secondary antibody: Fluorescein conjugated Anti-GFP at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.

Western Blot: GFP Antibody [NB600-303] - WB analysis of GFP in various lysates. Image courtesy of product review by Emilie Leclerc.

Immunoprecipitation: GFP Antibody [NB600-303] - IP analysis of GFP in HeLa cell lysate. Image courtesy of anonymous customer review.

Western Blot: GFP Antibody [NB600-303] - Analysis of GFP HRP expression in HeLa/GFP cell lysate.

Western Blot: GFP Antibody [NB600-303] - Analysis using Biotin conjugate of NB600-303. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti- Biotin Conjugated secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting for 45 min at RT. HRP Streptavidin was used at 1:40,000 in incubated with blocking buffer for 30 min at 20C. Block: 5% Blotto 30 min at 20C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.

Immunohistochemistry: GFP Antibody [NB600-303] - Eight-week-old Lgr5-GFP mice were injected with CCL4 and rHGF or/and rRspo1 proteins (0.1 ug/mice/time), as indicated in a, Lgr5 expression was measured using an immunofluorescent assay with anti-GFP antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01341-6) licensed under a CC-BY license.

Western Blot: GFP Antibody [NB600-303] - Lane 1: 293FT cells transfected with CDK4 dominant negative. Lane 2: 293FT cells poitive control. Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for GFP.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-303]

Western Blot of GFP Antibody. Lane 1: 293FT cells transfected with CDK4 dominant negative. Lane 2: 293FT cells poitive control. Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for GFP.

Western Blot of Rabbit anti-GFP antibody. Lane 1: Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) Goat-a-Rabbit IgG [H&L] MX10 () at 1:10,000 for 45 min at RT. Block: 5% BLOTTO in PBS overnight at 4C. Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.

The polo-boxes of Plk4 are not sufficient to mediate STIL binding.(A) Scheme of Flag-Plk4 fragments. (B) After overexpression of Flag-Plk4-fragments (A) and GFP-STIL in HEK293T cells, cell lysates were subjected to immunoprecipitations using anti-Flag antibodies. Coprecipitation of GFP-STIL with Flag-Plk4 fragments was detected by western blotting using anti-GFP and anti-Flag antibodies.

Lgr5+ liver stem cells transplantation decreased liver fibrosis. b) Lgr5+ cells were isolated from Lgr5-GFP mice treated with CCL4 by FACS assay for transplantation (left). The liver Lgr5 expression was stained using anti-GFP antibody in mice with Lgr5+ cells or PH transplantation (right) on day 40. n = 10 mice, scale bars, 200 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29079780), licensed under a CC-BY licence.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	NB100-1365
FITC	NB100-1183-0.025mg
FITC	NB100-1183

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NBP2-22111

GFP Antibody (4B10B2) - BSA Free

(2 Publications)

0.1 ml

NBP2-22111

Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Expression of GFP in HEK293T cells. This image was submitted via customer Review. Image using the HRP form of this antibody.

Immunocytochemistry/Immunofluorescence: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb .

Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).

Flow Cytometry: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb .

Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2).

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22111AF350
Alexa Fluor 405	NBP2-22111AF405
Alexa Fluor 488	NBP2-22111AF488
Alexa Fluor 532	NBP2-22111AF532
Alexa Fluor 594	NBP2-22111AF594
Alexa Fluor 647	NBP2-22111AF647
Alexa Fluor 700	NBP2-22111AF700
Alexa Fluor 750	NBP2-22111AF750
Allophycocyanin	NBP2-22111APC
Allophycocyanin/Cy7	NBP2-22111APCCY7
Biotin	NBP2-22111B
DyLight 350	NBP2-22111UV
DyLight 405	NBP2-22111V
DyLight 488	NBP2-22111G
DyLight 550	NBP2-22111R
DyLight 594	NBP2-22111DL594
DyLight 650	NBP2-22111C
DyLight 680	NBP2-22111FR
DyLight 755	NBP2-22111IR
FITC	NBP2-22111F
HRP	NBP2-22111H
Janelia Fluor 549	NBP2-22111JF549
Janelia Fluor 646	NBP2-22111JF646
PE	NBP2-22111PE
PE/Atto594	NBP2-22111PEATT594
PE/Cy5.5	NBP2-22111PECY55
PE/Cy7	NBP2-22111PECY7
PerCP	NBP2-22111PCP
mFluor Violet 450 SE	NBP2-22111MFV450
mFluor Violet 500 SE	NBP2-22111MFV500
mFluor Violet 610 SE	NBP2-22111MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22111SS
Azide and BSA Free	NBP2-80750

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NBP2-43575

GFP Antibody (859)

(29 Publications)

0.1 ml

NBP2-43575

Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody (859) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GFP Antibody (859) [NBP2-43575] - GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: GFP stained by GFP antibody [859] diluted at 1:2000.Blue: Hoechst 33342 staining.

Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [859] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GFP Antibody (859) [NBP2-43575] - Analysis of GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody diluted at 1:2000. Blue: Hoechst 33342 staining.

ELISA: GFP Antibody (859) [NBP2-43575] - Coating Ab: mouse anti-GFP (2ug/well) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP (1:20000)

Immunoprecipitation: GFP Antibody (859) [NBP2-43575] - Analysis of 400 ug cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 ug of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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AF4240

GFP Antibody [Unconjugated]

(1 Review)

(17 Publications)

100 μg

AF4240

Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with eGFP-tagged EDG6. PVDF membrane was probed with 1 µg/mL of Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GFP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GFP was detected in immersion fixed HEK293 human embryonic kidney cells transfected with GFP (green) using Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, middle panel; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of GFP-positive cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

HEK293 human embryonic kidney cells transfected with GFP was stained with Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody only (A, Catalog # F0108) or with Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240) followed by Secondary Antibody (B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. Quadrant markers were set based on control antibody staining (Catalog #AB‑108‑C).

Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line either mock transfected (-) or transfected with eGFP-tagged EDG6 (+), loaded at 0.2 mg/mL. A specific band was detected for GFP at approximately 114 kDa (as indicated) using 2.5 µg/mL of Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

All-Multi

Species Glossary

Reactivity: All-Multi

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Allophycocyanin	IC4240A
Phycoerythrin	IC4240P

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC4240P
Carrier Free	IC4240A
Carrier Free	AF4240-SP

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NB100-62622

GFP Antibody

(1 Review)

(8 Publications)

0.5 ml

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-37821

GFP Antibody

(1 Review)

(1 Publication)

100 μg

NBP2-37821

Western Blot: eGFP Antibody [NBP2-37821] - WB analysis on HEK293 cells transfected with Green Fluorescent Protein (GFP) using eGFP antibody. 20ug whole cells lyate were loaded. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: eGFP Antibody [NBP2-37821] - Analysis of eGFP using either natural fluorescence (green) or an eGFP antibody (red) in U2OS cells transfected with an EGFR-eGFP fusion protein.

Western Blot: eGFP Antibody [NBP2-37821] - 12% SDS-PAGE of HEK-293 cells transfected with pCMV-Myc-eGFP (0.25ug DNA lanes 2 and 4; 0.50ug DNA lanes 3 and 5) Coomassie stain (lanes 2 and 3) and western blot (lanes 4 and 5) with anti-eGFP antibody and goat anti-rabbit HRP secondary

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB42402

GFP Antibody (1025527) [Unconjugated]

100 μg

MAB42402

Western blot shows synthetic eGFP, recombinant RANGAP-1 tagged with eYFP, recombinant GRPuv, and crystal jelly GFP. PVDF membrane was probed with 2 µg/mL of Mouse Anti-GFP Monoclonal Antibody (Catalog # MAB42402) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GFP at approximately 28 and recombinant RANGAP-1 tagged with eYFP at 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

GFP was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green; right panel) using Mouse Anti-GFP Monoclonal Antibody (Catalog # MAB42402) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, left panel; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

HEK293 human embryonic kidney cell line transfected with protein tagged with either eGFP (filled histogram) or mCherry (open histogram) was stained with Mouse Anti-GFP Monoclonal Antibody (Catalog # MAB42402) or Mouse IgG2B Isotype Control (MAB0041, data not shown) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Simple Western lane view shows eGFP and recombinant human RANGAP-1 tagged with eYFP, loaded at 0.2 mg/mL. Specific bands were detected for GFP at approximately 35 kDa and recombinant human RANGAP-1 tagged with eYFP at approximately 51 kDa (as indicated) using 10 µg/mL of Mouse Anti-GFP Monoclonal Antibody (Catalog # MAB42402) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

All-Multi

Species Glossary

Reactivity: All-Multi

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC42402U-100UG
Alexa Fluor 405	IC42402V-100UG
Alexa Fluor 488	IC42402G-100UG
Alexa Fluor 532	FAB42402X
Alexa Fluor 594	IC42402T-100UG
Alexa Fluor 647	IC42402R-100UG
Alexa Fluor 700	IC42402N-100UG
Alexa Fluor 750	IC42402S-100UG
Allophycocyanin	FAB42402A
Biotin	FAB42402B
DyLight 350	FAB42402D
DyLight 405	FAB42402E
DyLight 488	FAB42402K
DyLight 550	FAB42402L
DyLight 594	FAB42402M
DyLight 650	FAB42402W
DyLight 680	FAB42402Y
DyLight 755	FAB42402Z
FITC	FAB42402F
HRP	FAB42402H
Janelia Fluor 549	FAB42402I
Janelia Fluor 646	FAB42402J
PE	FAB42402P
PerCP	FAB42402C
mFluor Violet 450 SE	FAB42402MFV450
mFluor Violet 500 SE	FAB42402MFV500
mFluor Violet 610 SE	FAB42402MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC42402T-100UG
Carrier Free	IC42402R-100UG
Carrier Free	IC42402U-100UG
Carrier Free	IC42402V-100UG
Carrier Free	IC42402G-100UG
Carrier Free	IC42402N-100UG
Carrier Free	IC42402S-100UG
Carrier Free	MAB42402-SP

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NB100-56401

GFP Antibody

(2 Publications)

0.1 mg

NB100-56401

Western Blot: GFP Antibody [NB100-56401] - Analysis of extracts from 293A cells, unstransfected (lane 1) and transfected (lane 2) with GFP expression vector, using GFP antibody (NB100-56401) at 1:1000 (0.5 ug/mL).

Western Blot: GFP Antibody [NB100-56401] - Analysis of GFP in 20 ug of transfected HeLa cells (A) and untransfected HeLa cells (B) using GFP antibody at 0.5 ug/mL.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5126A

Alt Catalog# IMG-5126A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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