![Western Blot: eGFP Antibody [NBP2-37821] - WB analysis on HEK293 cells transfected with Green Fluorescent Protein (GFP) using eGFP antibody. 20ug whole cells lyate were loaded. Image from verified customer review.](http://images.novusbio.com/fullsize/eGFP-Antibody-Western-Blot-NBP2-37821-img0004.jpg "Western Blot: eGFP Antibody [NBP2-37821] - WB analysis on HEK293 cells transfected with Green Fluorescent Protein (GFP) using eGFP antibody. 20ug whole cells lyate were loaded. Image from verified customer review.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | This GFP antibody was developed against purified recombinant eGFP |
| Specificity | GFP antibody detects eGFP in samples. This product has been successfully used in Western Blot and ICC/IF applications. The immunogen is purified recombinant eGFP. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C in the dark. |
| Buffer | PBS (pH 7.2) with 0.1% gelatin |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-37821 | Applications | Species |
|---|---|---|
| Franco-Iborra S, Cuadros T, Parent A et al. Defective mitochondrial protein import contributes to complex I-induced mitochondrial dysfunction and neurodegeneration in Parkinson's disease. Cell Death Dis. 2018-11-07 [PMID: 30405116] (WB, Jellyfish) | WB | Jellyfish |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Non-species related | 02/02/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
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How to visualize autophagy by microscopy By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to... Read full blog post. |
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Animal Models to Study Autophagy By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the... Read full blog post. |
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The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura. It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol... Read full blog post. |
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GFP - Be Green! Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Application: | WB | |
| Species: | Non-species related |
![Immunocytochemistry/Immunofluorescence: eGFP Antibody [NBP2-37821] - Analysis of eGFP using either natural fluorescence (green) or an eGFP antibody (red) in U2OS cells transfected with an EGFR-eGFP fusion protein.](http://images.novusbio.com/fullsize/eGFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37821-img0003.jpg "Immunocytochemistry/Immunofluorescence: eGFP Antibody [NBP2-37821] - Analysis of eGFP using either natural fluorescence (green) or an eGFP antibody (red) in U2OS cells transfected with an EGFR-eGFP fusion protein.")
![Western Blot: eGFP Antibody [NBP2-37821] - 12% SDS-PAGE of HEK-293 cells transfected with pCMV-Myc-eGFP (0.25ug DNA lanes 2 and 4; 0.50ug DNA lanes 3 and 5) Coomassie stain (lanes 2 and 3) and western blot (lanes 4 and 5) with anti-eGFP antibody and goat anti-rabbit HRP secondary](http://images.novusbio.com/fullsize/eGFP-Antibody-Western-Blot-NBP2-37821-img0001.jpg "Western Blot: eGFP Antibody [NBP2-37821] - 12% SDS-PAGE of HEK-293 cells transfected with pCMV-Myc-eGFP (0.25ug DNA lanes 2 and 4; 0.50ug DNA lanes 3 and 5) Coomassie stain (lanes 2 and 3) and western blot (lanes 4 and 5) with anti-eGFP antibody and goat anti-rabbit HRP secondary")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
