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5 Results for "novus"

MAB766

FGFR3 Antibody (136334) [Unconjugated]

(1 Review)

(4 Publications)

100 μg

MAB766

TAB cells modulate melanoma cells to express FGFR-3 and its ligand FGF-2 for tumor stroma-tumor cell cross-talk. a WM3749 co-cultured (72 h) with TAB cells (red bar) show increased FGFR-3 mRNA expression when compared with tumors only (open bar) or co-cultured with NB cells (blue bar). b Lysates obtained from pools of melanomas co-cultured (72–120 h) with NB- or TAB cells were probed in western blot with anti-FGFR-3 antibody (left panel), results expressed as relative intensity after beta -actin normalization (right panel). c Melanoma cells co-cultured with TAB cells (72 h) show increased phospho-FGFR-3 expression (right panel; immunofluorescence assays) when compared with melanoma cells alone (left panel) or melanoma cells co-cultured with NB cells (middle panel), scale bars: 40 μm, images captured by Nikon inverted microscope. d Melanoma cells co-cultured with TAB cells (red bar) show increased FGF-2 mRNA expression when compared with melanoma cells alone (open bar) or melanoma cells co-cultured with NB cells (blue bar). e 451Lu and WM989treated with IGF-1 (25 ng/ml/daily for 5 days; red bar) show increased FGFR-3 expression when compared with untreated controls (blue bar), flow cytometry results expressed as net % expression of control antibody. IGF-1 treated melanoma cells (red bars) show higher expression of FGFR-3 compared with untreated cells (blue bars). Bar represents mean + SD of replicate samples. f NB cells treated with FGF-2 (10 ng/ml/daily for 4 days; red bar) show high IGF-1 mRNA expression relative to untreated NB cells (blue bar). g 451Lu and WM989 co-cultured (72 h) with TAB cells in the presence of an anti-IGF-1 neutralizing antibody (10 μg/ml) show decreased FGFR-3 mRNA expression in tumor cells (blue bar) when compared with controls (red bar). h TAB cells co-cultured (72 h) with 451Lu and WM989 in the presence of an anti-FGF-2 neutralizing antibody (1 μg/ml) show decreased IGF-1 mRNA expression in B cells (blue bar) when compared with controls (red bar).Experiments in a, d and f–h were performed using qPCR. In Figures a, d–h, bars represent mean + SE of duplicate samples and are representative of at least two independent experiments. i Summary of cross-talk between melanoma and B cells: FGF-2 is constitutively expressed by tumor cells, released into the microenvironment to bind FGFR-3 on the B cells, activated B cells express increased levels of pro-inflammatory cytokines. IGF-1 released by TAB cells modulates tumor cells to increase their growth, heterogeneity and therapy resistance Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28928360), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB766U-100UG
Alexa Fluor 405	FAB766V-100UG
Alexa Fluor 488	FAB766G-100UG
Alexa Fluor 532	FAB766X
Alexa Fluor 594	FAB766T-100UG
Alexa Fluor 647	FAB766R-100UG
Alexa Fluor 700	FAB766N-100UG
Alexa Fluor 750	FAB766S-100UG
Allophycocyanin	FAB766A
Allophycocyanin/Cy7	FAB766APCCY7
Biotin	FAB766B
DyLight 350	FAB766D
DyLight 405	FAB766E
DyLight 488	FAB766K
DyLight 550	FAB766L
DyLight 594	FAB766M
DyLight 650	FAB766W
DyLight 680	FAB766Y
DyLight 755	FAB766Z
Fluorescein	FAB766F
HRP	FAB766H
Janelia Fluor 549	FAB766I
Janelia Fluor 646	FAB766J
PE/Atto594	FAB766PEATT594
PE/Cy5.5	FAB766PECY55
PE/Cy7	FAB766PECY7
Phycoerythrin	FAB766P
mFluor Violet 450 SE	FAB766MFV450
mFluor Violet 500 SE	FAB766MFV500
mFluor Violet 610 SE	FAB766MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB766F
Carrier Free	FAB766P
Carrier Free	FAB766A
Carrier Free	MAB766-SP
Carrier Free	MAB766-500
Carrier Free	FAB766V-100UG
Carrier Free	FAB766U-100UG
Carrier Free	FAB766T-100UG
Carrier Free	FAB766S-100UG
Carrier Free	FAB766R-100UG
Carrier Free	FAB766N-100UG
Carrier Free	FAB766G-100UG

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NBP2-66840

FGFR3 Antibody (JM110-33)

(1 Publication)

100 ul

NBP2-66840

Western Blot: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of FGFR3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: HepG2 cell lysateLane 3: 293 cell lysateLane 4: MCF-7 cell lysate

Immunocytochemistry/Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840] - Staining FGFR3 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded mouse testis tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.

Flow Cytometry: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of A549 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary

Immunocytochemistry/Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840] - Staining FGFR3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840] - Staining FGFR3 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded human kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded mouse brain tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded mouse kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-52468

FGFR3 Antibody (2H10B4) - BSA Free

(1 Publication)

0.1 mg

NBP2-52468

Western Blot: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis using FGFR3 mAb against human FGFR3 (AA: 529-694) recombinant protein. (Expected MW is 44.1 kDa)

Immunohistochemistry: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis of ovarian cancer tissues using FGFR3 mouse mAb with DAB staining.

Flow Cytometry: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis of A549 cells using FGFR3 mouse mAb (green) and negative control (red).

Western Blot: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis using FGFR3 mAb against HEK293 (1) and FGFR3 (AA: 529-694)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis of endometrial cancer tissues using FGFR3 mouse mAb with DAB staining.

ELISA: FGFR3 Antibody (2H10B4) [NBP2-52468] - Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-52468-0.025mg

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NBP3-21595

FGFR3 [p Tyr724] Antibody (SR2305)

100 ul

NBP3-21595

Western Blot: FGFR3 [p Tyr 724] Antibody (SR2305) [NBP3-21595] - Western blot analysis using NBP3-21595 in (1) MCF7 treated with pervanadate cell lysate; (2) untreated.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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FAB766F

FGFR3 Antibody (136334) [Fluorescein]

(1 Review)

100 Tests

FAB766F

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human FGF R3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB766F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Fluorescein

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB766U-100UG
Alexa Fluor 405	FAB766V-100UG
Alexa Fluor 488	FAB766G-100UG
Alexa Fluor 532	FAB766X
Alexa Fluor 594	FAB766T-100UG
Alexa Fluor 647	FAB766R-100UG
Alexa Fluor 700	FAB766N-100UG
Alexa Fluor 750	FAB766S-100UG
Allophycocyanin	FAB766A
Biotin	FAB766B
DyLight 350	FAB766D
DyLight 405	FAB766E
DyLight 488	FAB766K
DyLight 550	FAB766L
DyLight 594	FAB766M
DyLight 650	FAB766W
DyLight 680	FAB766Y
DyLight 755	FAB766Z
HRP	FAB766H
Janelia Fluor 549	FAB766I
Janelia Fluor 646	FAB766J
Phycoerythrin	FAB766P
mFluor Violet 450 SE	FAB766MFV450
mFluor Violet 500 SE	FAB766MFV500
mFluor Violet 610 SE	FAB766MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB766P
Carrier Free	FAB766A
Carrier Free	MAB766-SP
Carrier Free	MAB766-500
Carrier Free	MAB766-100
Carrier Free	FAB766V-100UG
Carrier Free	FAB766U-100UG
Carrier Free	FAB766T-100UG
Carrier Free	FAB766S-100UG
Carrier Free	FAB766R-100UG
Carrier Free	FAB766N-100UG
Carrier Free	FAB766G-100UG

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