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![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249403823.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249374927.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249351323.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249393597.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-212024940823.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249403890.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-212024940846.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03] - Analysis of ALPI expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3A8-Western-Blot-H00000248-M03-img0003.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03] - Analysis of ALPI expression in HeLa (Cat # L013V1).")
![ELISA: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03] - Detection limit for recombinant GST tagged ALPI is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3A8-ELISA-H00000248-M03-img0002.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0004.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.")
![Flow Cytometry: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Flow-Cytometry-NBP2-37387-img0005.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0002.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0003.jpg)
![ELISA: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-ELISA-NBP2-37387-img0001.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Western Blot of Rabbit anti-Alkaline Phosphatase antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (calf intestine). Load: 50 ng per lane.Primary antibody: Alkaline Phosphatase antibody Biotin conjugated at 1:2,000 for 60 min at RT.Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 57 kDa, 57 kDa for Alkaline Phosphatase.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Intestinal-Antibody-Western-Blot-NB120-7325-img0007.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Western Blot of Rabbit anti-Alkaline Phosphatase antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (calf intestine). Load: 50 ng per lane.Primary antibody: Alkaline Phosphatase antibody Biotin conjugated at 1:2,000 for 60 min at RT.Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 57 kDa, 57 kDa for Alkaline Phosphatase.")
![Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Lane 1: Alkaline Phosphatase (Calf Intestine). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (Calf Intestine) Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 57 kDa, 60 kDa for Alkaline Phosphatase (Calf Intestine).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Intestinal-Antibody-Western-Blot-NB120-7325-img0001.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Lane 1: Calf intestine Alkaline Phosphatase. Load: 1.5 ug Block: 5% Blotto 1 hour at 4C. Primary antibody: diluted to 1:1000 in 5% Blotto overnight at 4C. Secondary antibody: Goat anti Rabbit 1:20,000 in 1 Hour 4C Substrate: Femtomax 110.](http://images.novusbio.com/fullsize/Alkaline Phosphatase- Intestinal Antibody-Western Blot-NB120-7325-img0008.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB600-588] - Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-Western-Blot-NB600-588-img0004.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB600-588] - Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops.")
![Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) [NBP2-68196] - Standard Curve Reference](http://images.novusbio.com/fullsize/Rat-Alkaline-Phosphatase-Intestinal-ELISA-Kit-Colorimetric-img0001.jpg "Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) [NBP2-68196] - Standard Curve Reference")
![ELISA: Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) [NBP2-68196] - Samples were spiked with high concentrations of Rat Alkaline Phosphatase, Intestinal and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68196_rat-alkaline-phosphatase-intestinal-elisa-kit-color-132202416204067.jpg)
![ELISA: Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) [NBP2-68196] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68196_rat-alkaline-phosphatase-intestinal-elisa-kit-color-4320249173450.jpg)
![SDS-Page: Recombinant Human Alkaline Phosphatase, Intestinal Protein [H00000248-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Alkaline-Phosphatase--Intestinal-Protein-SDS-Page-H00000248-Q01-img0002.jpg "SDS-Page: Recombinant Human Alkaline Phosphatase, Intestinal Protein [H00000248-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (SR2122) [NBP3-21913] - Western blot analysis using NBP3-21913 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21913_rabbit-alkaline-phosphatase-intestinal-mab-sr2122-57202315545115.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (SR2122) [NBP3-21913] - Western blot analysis using NBP3-21913 in HeLa cell lysate.")
![Immunohistochemistry: Alkaline Phosphatase, Intestinal Antibody [NBP2-34057] - Staining of human small intestine shows distinct membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Intestinal-Antibody-Immunohistochemistry-NBP2-34057-img0003.jpg "Immunohistochemistry: Alkaline Phosphatase, Intestinal Antibody [NBP2-34057] - Staining of human small intestine shows distinct membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody [NBP2-33624] - Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Intestinal-Antibody-Immunohistochemistry-Paraffin-NBP2-33624-img0002.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody [NBP2-33624] - Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.")