Hu,
Mu,
Rt,
Po,
Am,
Ca,
Ch,
Fe,
Ha,
Pm,
Rb,
ShSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Am, Ca, Ch, Fe, Ha, Pm, Rb, Sh
WB, Flow, ICC/IF, IHC, IP
Applications: WB, Flow, ICC/IF, IHC, IP
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Western-Blot-NB300-578-img0015.jpg "Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).")
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in U251 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0006.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0014.jpg)
![Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Hela cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Flow-Cytometry-NB300-578-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in C6 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in Hela Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0005.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0007.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0012.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0013.jpg)
![Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Jurkat cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Flow-Cytometry-NB300-578-img0010.jpg)
![Western Blot: FAM50A Antibody (5F10) [H00009130-M02] - FAM50A monoclonal antibody (M02), clone 5F10. Analysis of FAM50A expression in PC-12.](http://images.novusbio.com/fullsize/FAM50A-Antibody-5F10-Western-Blot-H00009130-M02-img0008.jpg "Western Blot: FAM50A Antibody (5F10) [H00009130-M02] - FAM50A monoclonal antibody (M02), clone 5F10. Analysis of FAM50A expression in PC-12.")
![Immunohistochemistry-Paraffin: FAM50A Antibody (5F10) [H00009130-M02] - Analysis of monoclonal antibody to FAM50A on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 0.5 ug/ml.](http://images.novusbio.com/fullsize/FAM50A-Antibody-5F10-Immunohistochemistry-Paraffin-H00009130-M02-img0006.jpg)
![Western Blot: FAM50A Antibody (5F10) [H00009130-M02] - FAM50A monoclonal antibody (M02), clone 5F10 Analysis of FAM50A expression in Hela S3 NE.](http://images.novusbio.com/fullsize/FAM50A-Antibody-5F10-Western-Blot-H00009130-M02-img0009.jpg)
![Sandwich ELISA: FAM50A Antibody (5F10) [H00009130-M02] - Detection limit for recombinant GST tagged FAM50A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FAM50A-Antibody-5F10-Sandwich-ELISA-H00009130-M02-img0007.jpg)
![Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-262202415355720.jpg "Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-27220248471912.jpg)
![Western Blot: ATPB Antibody (5F10) [NBP3-26490] - Positive Western Blot detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue. All lanes: ATPB Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57 kDa Observed band size: 57 kDa](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-282202411363015.jpg)
![Immunohistochemistry: beta-NGF Antibody (5F10) [NBP3-26112] - Image of beta-NGF Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26112_rabbit-beta-ngf-mab-5f10-262202415355740.jpg "Immunohistochemistry: beta-NGF Antibody (5F10) [NBP3-26112] - Image of beta-NGF Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: beta-NGF Antibody (5F10) [NBP3-26112] - Positive Western Blot detected in: U-87 whole cell lysate, Rat Brain whole cell lysate. All lanes: beta-NGF Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 27 kDa Observed band size: 39 kDa](http://images.novusbio.com/fullsize/nbp3-26112_rabbit-beta-ngf-mab-5f10-282202411363019.jpg)