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![Western Blot: ATM Antibody (2C1) [NB100-309] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:1000. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-Western-Blot-NB100-309-img0016.jpg "Western Blot: ATM Antibody (2C1) [NB100-309] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:1000. Theoretical molecular weight 351 kDa.")
![Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody (2C1) [NB100-309] at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-Immunohistochemistry-Paraffin-NB100-309-img0011.jpg)
![Western Blot: ATM Antibody (2C1) [NB100-309] - Detection of human ATM protein using ATM antibody (2C1) [NB100-309] by western blot or immunoprecipitation. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-Western-Blot-NB100-309-img0018.jpg)
![Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human breast carcinoma. ATM stained by ATM antibody [2C1] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-Immunohistochemistry-Paraffin-NB100-309-img0021.jpg)
![Western Blot: ATM Antibody (2C1) [NB100-309] - Analysis of ATM in U2OS sarcoma cells using ATM antibody (2C1) [NB100-309]. Theoretical molecular weight 351 kDa. Western blot image submitted by a verifeid customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-Western-Blot-NB100-309-img0010.jpg)
![Western Blot: ATM Antibody (2C1) [NB100-309] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:1000. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-Western-Blot-NB100-309-img0015.jpg)
![Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody (2C1) [NB100-309] at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-Immunohistochemistry-Paraffin-NB100-309-img0012.jpg)
![Knockdown Validated: ATM Antibody (2C1) [NB100-309] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:500.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-Knockdown-Validated-NB100-309-img0017.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: ATM Antibody (2C1) [NB100-309] - Methylated EDSBs may be repaired by an ATM-dependent pathway. Immunoblots of ATM and DNA-PKcs in ATM shRNA-transfected HeLa cells, using ATM antibody (2C1) [NB100-309]. GAPDH is included as a loading control. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-9-70), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-Western-Blot-NB100-309-img0019.jpg)
![Immunoprecipitation: ATM Antibody (2C1) [NB100-309] - MDA-MB-231 whole cell lysates were immunoprecipitated with 2 ug ATM Antibody (NB100-309), followed by Western blot (primary antibody: NB100-309 at 1:1000, 4C overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-Western-Blot-NB100-309-img0020.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ATM Antibody (2C1) [NB100-309] - MCELNs decreased the DNA damage of H9C2 cells after radiation. Western blot images of p-ATM (NB100-306) and ATM (NB100-309) in H9C2 cells after 48 h of culture with indicated treatment. IR (-/+): 0/16 Gy X-ray; MCELNs (-/+): 0/10 ug/mL. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35509278/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb100-309_mouse-monoclonal-atm-antibody-2c1-25520231514910.jpg)
![Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Western-Blot-NB200-113-img0007.jpg "Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Samples: 1) Human Recombinant MMP-2, and 2)Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - NB200-113, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Western-Blot-NB200-113-img0009.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). MMP2 immunoreactivity was observed in the cytoplasm of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted MMP2). The cancer cells present in the core of tumor areas were found to be negative for MMP2 staining.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0006.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This MMP2 antibody generated an expected immunoreactivity of MMP2 protein in the cytoplasm of most of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted pool of MMP2). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0003.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Upper image: MMP-2 FITC (green), DAPI (blue)Lower image: phase contrast of human choroid plexus (Alzheimer's disease). Image from verified customer review.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0011.jpg)
![Flow Cytometry: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Flow Cytometry: MMP-2 Antibody (2C1) [Alexa Fluor® 405] [NB200-113AF405] - CD4+ T cells were activated for 24h before being fixed and permeabilized to detect MMP-2. Image using the Alexa Fluor 405 form of this antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Flow-Cytometry-NB200-113-img0012.jpg)
![Western Blot: BACE-1 Antibody (2C1) [H00023621-M01] - BACE1 monoclonal antibody (M02), clone 2C1. Analysis of BACE1 expression in rat testis.](http://images.novusbio.com/fullsize/BACE-1-Antibody-2C1-Western-Blot-H00023621-M01-img0005.jpg "Western Blot: BACE-1 Antibody (2C1) [H00023621-M01] - BACE1 monoclonal antibody (M02), clone 2C1. Analysis of BACE1 expression in rat testis.")
![Immunohistochemistry-Paraffin: BACE-1 Antibody (2C1) [H00023621-M01] - Analysis of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/BACE-1-Antibody-2C1-Immunohistochemistry-Paraffin-H00023621-M01-img0004.jpg)
![Western Blot: BACE-1 Antibody (2C1) [H00023621-M01] - Analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.Lane 1: BACE1 transfected lysate(52.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BACE-1-Antibody-2C1-Western-Blot-H00023621-M01-img0006.jpg)
![ELISA: BACE-1 Antibody (2C1) [H00023621-M01] - Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BACE-1-Antibody-2C1-ELISA-H00023621-M01-img0002.jpg)
![Proximity Ligation Assay: BACE-1 Antibody (2C1) [H00023621-M01] - Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/BACE-1-Antibody-2C1-Proximity-Ligation-Assay-H00023621-M01-img0003.jpg)
![Western Blot: FOXR2 Antibody (2C1) [H00139628-M01] - FOXR2 monoclonal antibody (M01), clone 2C1 Analysis of FOXR2 expression in Hela.](http://images.novusbio.com/fullsize/FOXR2-Antibody-2C1-Western-Blot-H00139628-M01-img0010.jpg "Western Blot: FOXR2 Antibody (2C1) [H00139628-M01] - FOXR2 monoclonal antibody (M01), clone 2C1 Analysis of FOXR2 expression in Hela.")
![Immunocytochemistry/Immunofluorescence: FOXR2 Antibody (2C1) [H00139628-M01] - Analysis of monoclonal antibody to FOXR2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/FOXR2-Antibody-2C1-Immunocytochemistry-Immunofluorescence-H00139628-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: FOXR2 Antibody (2C1) [H00139628-M01] - Analysis of monoclonal antibody to FOXR2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/FOXR2-Antibody-2C1-Immunohistochemistry-Paraffin-H00139628-M01-img0008.jpg)
![ELISA: FOXR2 Antibody (2C1) [H00139628-M01] - Detection limit for recombinant GST tagged FOXR2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FOXR2-Antibody-2C1-ELISA-H00139628-M01-img0011.jpg)
![Western Blot: SOX15 Antibody (2C1) [H00006665-M02] - SOX15 monoclonal antibody (M02), clone 2C1. Analysis of SOX15 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/SOX15-Antibody-2C1-Western-Blot-H00006665-M02-img0005.jpg "Western Blot: SOX15 Antibody (2C1) [H00006665-M02] - SOX15 monoclonal antibody (M02), clone 2C1. Analysis of SOX15 expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: SOX15 Antibody (2C1) [H00006665-M02] - Analysis of monoclonal antibody to SOX15 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SOX15-Antibody-2C1-Immunohistochemistry-Paraffin-H00006665-M02-img0003.jpg)
![Sandwich ELISA: SOX15 Antibody (2C1) [H00006665-M02] - Detection limit for recombinant GST tagged SOX15 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SOX15-Antibody-2C1-Sandwich-ELISA-H00006665-M02-img0004.jpg)
![Western Blot: KDEL Antibody (2C1) [NBP2-61926] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 (NBP2-61926). Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL (NBP2-61926). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101).. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61926) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.](http://images.novusbio.com/fullsize/KDEL-Antibody-2C1-Western-Blot-NBP2-61926-img0002.jpg "Western Blot: KDEL Antibody (2C1) [NBP2-61926] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 (NBP2-61926). Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL (NBP2-61926). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101).. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61926) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.")
![Immunohistochemistry: KDEL Antibody (2C1) [NBP2-61926] - Immunohistochemistry analysis using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 (NBP2-61926). Tissue: kidney. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61926) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) KDEL Antibody (D) Composite.](http://images.novusbio.com/fullsize/KDEL-Antibody-2C1-Immunohistochemistry-NBP2-61926-img0003.jpg)
![Immunohistochemistry-Paraffin: HERC3 Antibody (2C1) [H00008916-M01] - Analysis of monoclonal antibody to HERC3 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/HERC3-Antibody-2C1-Immunohistochemistry-Paraffin-H00008916-M01-img0004.jpg "Immunohistochemistry-Paraffin: HERC3 Antibody (2C1) [H00008916-M01] - Analysis of monoclonal antibody to HERC3 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.")
![Sandwich ELISA: HERC3 Antibody (2C1) [H00008916-M01] - Detection limit for recombinant GST tagged HERC3 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HERC3-Antibody-2C1-Sandwich-ELISA-H00008916-M01-img0005.jpg)
![Immunoprecipitation: Aconitase 1 Antibody (2C1) [H00000048-M01] - Analysis of ACO1 transfected lysate using anti-ACO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACO1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-2C1-Immunoprecipitation-H00000048-M01-img0002.jpg "Immunoprecipitation: Aconitase 1 Antibody (2C1) [H00000048-M01] - Analysis of ACO1 transfected lysate using anti-ACO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACO1 MaxPab rabbit polyclonal antibody.")
![ELISA: Aconitase 1 Antibody (2C1) [H00000048-M01] - Detection limit for recombinant GST tagged ACO1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-2C1-ELISA-H00000048-M01-img0003.jpg)
![Western Blot: ZIC3 Antibody (2C1) [H00007547-M05] - Analysis of ZIC3 expression in Y-79 (Cat # L042V1).](http://images.novusbio.com/fullsize/ZIC3-Antibody-2C1-Western-Blot-H00007547-M05-img0004.jpg "Western Blot: ZIC3 Antibody (2C1) [H00007547-M05] - Analysis of ZIC3 expression in Y-79 (Cat # L042V1).")
![Western Blot: ZIC3 Antibody (2C1) [H00007547-M05] - Analysis of ZIC3 expression in human Skeletal muscle.](http://images.novusbio.com/fullsize/ZIC3-Antibody-2C1-Western-Blot-H00007547-M05-img0005.jpg)
![ELISA: ZIC3 Antibody (2C1) [H00007547-M05] - Detection limit for recombinant GST tagged ZIC3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ZIC3-Antibody-2C1-ELISA-H00007547-M05-img0003.jpg)
![Western Blot: p38 gamma/SAPK3 Antibody (2C1) [H00006300-M04] - Analysis of MAPK12 expression in Hela (Cat # L013V1).](http://images.novusbio.com/fullsize/p38-gamma-SAPK3-Antibody-2C1-Western-Blot-H00006300-M04-img0006.jpg "Western Blot: p38 gamma/SAPK3 Antibody (2C1) [H00006300-M04] - Analysis of MAPK12 expression in Hela (Cat # L013V1).")
![Immunocytochemistry/Immunofluorescence: p38 gamma/SAPK3 Antibody (2C1) [H00006300-M04] - Analysis of monoclonal antibody to MAPK12 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/p38-gamma-SAPK3-Antibody-2C1-Immunocytochemistry-Immunofluorescence-H00006300-M04-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PFDN2 Antibody (2C1) [H00005202-M07] - Analysis of monoclonal antibody to PFDN2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PFDN2-Antibody-2C1-Immunocytochemistry-Immunofluorescence-H00005202-M07-img0001.jpg "Immunocytochemistry/Immunofluorescence: PFDN2 Antibody (2C1) [H00005202-M07] - Analysis of monoclonal antibody to PFDN2 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: PFDN2 Antibody (2C1) [H00005202-M07] - Detection limit for recombinant GST tagged PFDN2 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PFDN2-Antibody-2C1-Sandwich-ELISA-H00005202-M07-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MEFV Antibody (2C1) [H00004210-M02] - Analysis of monoclonal antibody to MEFV on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MEFV-Antibody-2C1-Immunocytochemistry-Immunofluorescence-H00004210-M02-img0002.jpg "Immunocytochemistry/Immunofluorescence: MEFV Antibody (2C1) [H00004210-M02] - Analysis of monoclonal antibody to MEFV on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: MEFV Antibody (2C1) [H00004210-M02] - Detection limit for recombinant GST tagged MEFV is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MEFV-Antibody-2C1-Sandwich-ELISA-H00004210-M02-img0003.jpg)