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NB300-221

GAPDH Antibody (1D4)

(19 Reviews)

(411 Publications)

0.1 ml

NB300-221

Analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.

Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDH antibody produces strong cytoplasmic staining of healthy cells.

Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.

Human eye cell lysate. Antibody used at 1:500. WB image submitted by a verified customer review.

Analysis of GAPDH in chicken pectoralis major using anti-GAPDH antibody. The primary antibody was used at a dilution of 1:4000, incubated for 1 hour at room temperature. Western blot image submitted by a verified customer review.

Application of the approach to other cell lines. The remainder of the protocol from Figure 2 was then applied using 100 ug/mL digitonin as the optimal cytosolic extract in each case. An aliquot of each extract was then analyzed by 4-12% SDS PAGE followed by Western blotting and probing with antibodies to GAPDH, BiP and Histone H3 (Used as an alternative nuclear marker due to weakness of the Lamin A signal in HeLa cells). Image collected and cropped by CiteAb from the following publication (//bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-2-243) licensed under a CC-BY license.

Endothelial responses provoked by Hb-lipid interactions are attenuated by sulfide donors. Confluence HAoECs were exposed to the obtained Hb samples for 8 hours and VCAM-1 expression was determined by Western blotting. GAPDH were used as a loading control. Representative experiment, n = 3. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2018/3812568/), licensed under a CC-BY license.

Regulation of cell migration-associated proteins by NCKU-21 in A549 and CL1-5 cells. Protein expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9 were analyzed in A549 cell treated with NCKU-21 for 24 hr. * P 80% of endogenous Sin1 while levels of myc-FL Sin1 associated with Rictor are comparable with endogenous Sin1 levels. Image collected and cropped by CiteAb from the following open publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.20086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunodepletions and the effects of RNases and various transcription inhibitors on chromosome condensation and cell cycle state.(A) Western blot for CAP-E, XPB, and Tubulin in Mock or CAP-E depleted extracts. Dilution series of Mock-depleted extracts was used to calculate the % depletion of CAP-E using quantitative fluorescence. (B) 1% agarose gel stained with SYBR safe stain showing RNase A dependent removal of total RNAs from high-speed supernatant egg extracts. (C) Representative fluorescence images of chromatid assembly at steady state (180 min after sperm addition) in the presence of indicated buffers and enzymes. See Materials and methods for details. Note that the high salt in the RNase H buffer causes morphological changes to chromatids regardless of activity. Mean condensation parameters for each condition are indicated in lower left corner of each image. (D) Western blot for XPB and Tubulin in IgG or XPB-depleted extracts. Dilution series of IgG-depleted extracts was used to calculate the % depletion of XPB using quantitative fluorescence. (E) Western blot for XPF, ERCC1, and Tubulin in IgG or ERCC1-depleted extracts. Dilution series of IgG-depleted extracts was used to calculate the % depletion of ERCC1 and XPF using quantitative fluorescence. (F) Representative fluorescence images of chromatid assembly at steady state (180 min after sperm addition) in the presence of DMSO, 30 μM CDK7 inhibitor THZ1, or 30 μM THZ1 and 50 μM triptolide (TPL). (G) Triptolide does not affect CDK1 activation or maintenance of M phase. Western blot for CDK1 T161ph, histone H3T3ph, and Tubulin in M phase HSS extracts in the presence of DMSO or 50 μM triptolide. Both CDK1T161ph (a CDK7 substrate) and H3T3ph are only highly phosphorylated in M phase, and their appearance is not affected by triptolide treatment.Figure 1—figure supplement 1—source data 1.Source data for Figure 1—figure supplement 1A, B,D, E, G.Source data for Figure 1—figure supplement 1A, B,D, E, G. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Activation of the ATM‐Chk2‐p53 pathway upon CDDP treatmentAConfocal images showing the basal region of organ of Corti cultures treated with either culture medium alone or medium containing 10 μM CDDP for 1 day and immunolabeled for myosin 7A (red) and p‐ATM (green). Scale bar = 15 μm.BHigher magnification images showing representative OHC and IHC nuclei from control and CDDP‐exposed cells. Scale bar = 5 μm.CHistograms displaying green fluorescent signal intensity of x‐projections of OHC and IHC nuclei from control and CDDP‐exposed cells.DQuantification analysis of p‐ATM foci numbers in both OHCs and IHCs exposed to either culture medium alone (light blue and red lines for OHCs and IHCs, respectively) or 10 μM CDDP (blue and red lines for OHCs and IHCs, respectively; n = 50 nuclei per condition and per time point). Data are expressed as mean ± SEM. One‐way ANOVA test followed by post hoc Tukey's test (**P < 0.008, ***P = 0.00005; CDDP‐exposed OHCs versus CDDP‐exposed IHCs).EHigher magnification confocal and 3D images showing the co‐localization of p‐ATM foci (green) with 53BP1 foci (red) in an OHC nucleus from the basal region of the organ of Corti culture treated with 10 μM CDDP for 1 day. Scale bar = 5 μm.FConfocal images showing the basal region of organ of Corti cultures treated with either culture medium alone or medium containing 10 μM CDDP for 1 day and immunolabeled for myosin 7A (red) and p‐Chk2 (green). Scale bar = 15 μm.GHistograms of green fluorescent signal intensity from OHC and IHC nuclei of a CDDP‐treated organ of Corti explant.HQuantification of Chk2‐positive nuclei in both IHCs (red bars) and OHCs (blue bars) from control and CDDP‐treated organ of Corti cultures (n = 50 nuclei per condition and per time point). Data are expressed as mean ± SEM. Kruskal–Wallis test followed by post hoc Dunn's test (*P = 0.045, ***P = 0.0002; CDDP‐exposed IHCs versus control IHCs, CDDP‐exposed OHCs versus control OHCs).IRepresentative Western blots using antibodies against p‐p53 (serine 15), p53, and beta ‐actin in control and 10 μM CDDP‐exposed whole cochlear extracts.J, KHistograms representing the levels of total p53 protein (J) and p53 phosphorylation (K) in control (gray bars) and CDDP‐exposed cochleae (green bars; n = 6 cochleae per condition and per time point). Actin served as a loading control. Data are expressed as mean ± SEM. One‐way ANOVA test followed by post hoc Tukey's test (J: *P ≤ 0.023; K: *P = 0.032, **P = 0.007, ***P ≤ 0.0008; cochleae at the different times after CDDP exposure versus control 12 h).Data information: All experiments were performed in triplicate. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27794029), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-47602

Rhodopsin Antibody (1D4)

(4 Publications)

0.1 mg

NBP1-47602

Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Quantification of rhodopsin protein (western blot) levels in the neural retina of WT (n = 7) and hFATP1TG (n = 8) mice. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28672005/) licensed under a CC-BY license.

Immunohistochemistry: Rhodopsin Antibody (1D4) [NBP1-47602] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (NBP1-47602). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP1-47602) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.

Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (NBP1-47602). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP1-47602) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Non-cell-autonomous effects of hFATP1 in the neural retina. (E) Quantification of rhodopsin mRNA (qPCR) and protein (western blot) levels in the neural retina of WT (n = 7) and hFATP1TG (n = 8) mice. (F) qPCR quantification of FATP1 mRNA levels in the neural retina of WT (n = 8) and transgenic (n = 7) mice. Data were normalized to actin mRNA and tubulin protein levels. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0180148), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, Bv, Vb

Species Glossary

Reactivity: Hu, Mu, Bv, Vb

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NBP1-47602AF405
Alexa Fluor 488	NBP1-47602AF488
Alexa Fluor 647	NBP1-47602AF647
Alexa Fluor 700	NBP1-47602AF700
Biotin	NBP1-47602B
DyLight 350	NBP1-47602UV
DyLight 405	NBP1-47602V
DyLight 488	NBP1-47602G
DyLight 550	NBP1-47602R
DyLight 650	NBP1-47602C
DyLight 680	NBP1-47602FR
DyLight 755	NBP1-47602IR
FITC	NBP1-47602F
HRP	NBP1-47602H
Janelia Fluor 549	NBP1-47602JF549
Janelia Fluor 646	NBP1-47602JF646

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H00001479-M01

CSTF3 Antibody (1D4)

(1 Publication)

0.1 mg

H00001479-M01

Western Blot: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4. Lane 1: CSTF3 transfected lysate(11.44 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of monoclonal antibody to CSTF3 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Western Blot: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi ( Cat # H00001479-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody (M01), clone 1D4 (Cat # H00001479-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: CSTF3 Antibody (1D4) [H00001479-M01] - Detection limit for recombinant GST tagged CSTF3 is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00030849-M01

PIK3R4 Antibody (1D4)

0.1 mg

H00030849-M01

Western Blot: PIK3R4 Antibody (1D4) [H00030849-M01] - PIK3R4 monoclonal antibody (M01), clone 1D4 Analysis of PIK3R4 expression in HeLa.

Western Blot: PIK3R4 Antibody (1D4) [H00030849-M01] - Analysis of PIK3R4 over-expressed 293 cell line, cotransfected with PIK3R4 Validated Chimera RNAi ( Cat # H00030849-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIK3R4 monoclonal antibody (M01) clone 1D4 (Cat # H00030849-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: PIK3R4 Antibody (1D4) [H00030849-M01] - Analysis of PIK3R4 expression in transfected 293T cell line by PIK3R4 monoclonal antibody (M01), clone 1D4.Lane 1: PIK3R4 transfected lysate(153.1 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: PIK3R4 Antibody (1D4) [H00030849-M01] - Detection limit for recombinant GST tagged PIK3R4 is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00023265-M01

EXOC7 Antibody (1D4)

0.1 mg

H00023265-M01

Western Blot: EXOC7 Antibody (1D4) [H00023265-M01] - EXOC7 monoclonal antibody (M01), clone 1D4 Analysis of EXOC7 expression in HeLa.

Immunohistochemistry-Paraffin: EXOC7 Antibody (1D4) [H00023265-M01] - Analysis of monoclonal antibody to EXOC7 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Sandwich ELISA: EXOC7 Antibody (1D4) [H00023265-M01] - Detection limit for recombinant GST tagged EXOC7 is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00007172-M02

TPMT Antibody (1D4)

0.1 mg

H00007172-M02

Western Blot: TPMT Antibody (1D4) [H00007172-M02] - Analysis of TPMT expression in transfected 293T cell line by TPMT monoclonal antibody (M02), clone 1D4. Lane 1: TPMT transfected lysate(28.2 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: TPMT Antibody (1D4) [H00007172-M02] - Analysis of monoclonal antibody to TPMT on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: TPMT Antibody (1D4) [H00007172-M02] - Detection limit for recombinant GST tagged TPMT is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00002517-M01

Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4)

(1 Publication)

0.1 mg

H00002517-M01

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) [H00002517-M01] - Analysis of FUCA1 expression in transfected 293T cell line by FUCA1 monoclonal antibody (M01), clone 1D4. Lane 1: FUCA1 transfected lysate(53.2 KDa). Lane 2: Non-transfected lysate.

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) [H00002517-M01] - FUCA1 monoclonal antibody (M01), clone 1D4 Western Blot analysis of FUCA1 expression in K-562 ( Cat # L009V1 ).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00002303-M16

FoxC2 Antibody (1D4)

(1 Publication)

50 μg

H00002303-M16

Immunocytochemistry/Immunofluorescence: FoxC2 Antibody (1D4) [H00002303-M16] - Analysis of monoclonal antibody to FOXC2 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: FoxC2 Antibody (1D4) [H00002303-M16] - Detection limit for recombinant GST tagged FOXC2 is 0.1 ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00283431-M01

GAS2L3 Antibody (1D4)

(1 Publication)

0.1 mg

H00283431-M01

Sandwich ELISA: GAS2L3 Antibody (1D4) [H00283431-M01] - Detection limit for recombinant GST tagged GAS2L3 is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00051043-M03-100ug

Th-POK Antibody (1D4)

100 μg

H00051043-M03-100ug

Western Blot: Th-POK Antibody (1D4) [H00051043-M03-100ug] - Detection against Immunogen (37.29 KDa) .

Western Blot: Th-POK Antibody (1D4) [H00051043-M03-100ug] - Analysis of ZBTB7B expression in HeLa.

ELISA: Th-POK Antibody (1D4) [H00051043-M03-100ug] - Detection limit for recombinant GST tagged ZBTB7B is 0.03 ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00001758-M01

DMP-1 Antibody (1D4)

0.1 mg

H00001758-M01

Immunocytochemistry/Immunofluorescence: DMP-1 Antibody (1D4) [H00001758-M01] - Analysis of monoclonal antibody to DMP1 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: DMP-1 Antibody (1D4) [H00001758-M01] - Detection limit for recombinant GST tagged DMP1 is 1 ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00112802-M02

Cytokeratin 71 Antibody (1D4)

0.1 mg

H00112802-M02

Western Blot: Cytokeratin 71 Antibody (1D4) [H00112802-M02] - Analysis of KRT71 expression in transfected 293T cell line by KRT71 monoclonal antibody (M02), clone 1D4.Lane 1: KRT71 transfected lysate (Predicted MW: 57.2 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: Cytokeratin 71 Antibody (1D4) [H00112802-M02] - Detection limit for recombinant GST tagged KRT71 is 0.1 ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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