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NBP1-92687

Vimentin Antibody (2D1)

(1 Review)

(10 Publications)

0.1 ml

NBP1-92687

Knockout Validated: Vimentin Antibody (2D1) [NBP1-92687] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.

Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Analysis of different cell lysates using mouse mAb to vimentin, NBP1-92687, dilution 1:10,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, and [6] C6 cells. The band at about 50kDa mark corresponds to the vimentin protein.

Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - Analysis of cortical neuron-glial cell cultures from E20 rat stained with mouse mAb to vimentin, NBP1-92687, dilution 1:2,000 in red, and costained with chicken pAb to glial fibrillary acidic protein (GFAP), dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Fibroblastic and other developing cells express only vimentin and appear red. Astrocytes that express GFAP only are green while those that express both GFAP and vimentin appear golden yellow.

Immunohistochemistry-Paraffin: Vimentin Antibody (2D1) [NBP1-92687] - Human breast cancer tissue stained for Vimentin (white) and countersatined with DAPI (blue). Alexa Fluor 750 version of antibody used (NBP1-92687AF750). Image from verified customer review.

Immunohistochemistry: Vimentin Antibody (2D1) [NBP1-92687] - Mouse cortex microslice section (from postnatal day 3) was immunostained with (1:500 dil.). This image was submitted via customer Review.

Simple Western: Vimentin Antibody (2D1) [NBP1-92687] - Simple Western lane view shows a specific band for Vimentin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

High glucose (HG) concentrations induced epithelial-to-mesenchymal transition protein expression and enhanced migration activity in colorectal cancer (CRC) cells. SW480 (low metastatic potential) and SW620 (high metastatic potential) cells were cultured in different concentrations of glucose (normal: NG; HG; and osmotic control: NG + l-glucose). (B) HG concentration caused downregulation of E-cadherin and upregulation of N-cadherin, betaCTN, and vimentin, but c-myc was unchanged, as detected using Western blotting. beta-actin was evaluated as an internal control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30965609), licensed under a CC-BY licence.

Reactivity:

Hu, Rt, Mu(-)

Species Glossary

Reactivity: Hu, Rt, Mu(-)

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-92687AF350
Alexa Fluor 405	NBP1-92687AF405
Alexa Fluor 488	NBP1-92687AF488
Alexa Fluor 532	NBP1-92687AF532
Alexa Fluor 594	NBP1-92687AF594
Alexa Fluor 647	NBP1-92687AF647
Alexa Fluor 700	NBP1-92687AF700
Alexa Fluor 750	NBP1-92687AF750
Biotin	NBP1-92687B
DyLight 350	NBP1-92687UV
DyLight 405	NBP1-92687V
DyLight 488	NBP1-92687G
DyLight 550	NBP1-92687R
DyLight 594	NBP1-92687DL594
DyLight 650	NBP1-92687C
DyLight 680	NBP1-92687FR
DyLight 755	NBP1-92687IR
FITC	NBP1-92687F
HRP	NBP1-92687H
Janelia Fluor 549	NBP1-92687JF549
Janelia Fluor 646	NBP1-92687JF646
mFluor Violet 450 SE	NBP1-92687MFV450
mFluor Violet 500 SE	NBP1-92687MFV500
mFluor Violet 610 SE	NBP1-92687MFV610

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NBP2-43560

Vimentin Antibody (812)

(5 Publications)

0.1 ml

NBP2-43560

Knockout Validated: Vimentin Antibody (812) [NBP2-43560] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (812) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Frozen sectioned E13.5 Rat brain. Green: Vimentin protein stained by Vimentin antibody [812] diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Analysis of Frozen section of embryonic mouse brain (mE18.5). Red: Vimentin antibody [812] diluted at 1:250. Blue: DAPI.

Flow Cytometry: Vimentin Antibody (812) [NBP2-43560] - An intracellular stain was performed on Jurkat cells with NBP2-43560AF00 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Western Blot: Vimentin Antibody (812) [NBP2-43560] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [812] ) diluted at 1:75000.

Immunohistochemistry-Paraffin: Vimentin Antibody (812) [NBP2-43560] - Rat ovary. Vimentin antibody [812] diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Vimentin Antibody (812) [NBP2-43560] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract 10 % SDS-PAGE Vimentin antibody [812] dilution: 1:1000

Western Blot: Vimentin Antibody (812) [NBP2-43560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [812] diluted at 1:3000.

Western Blot: Vimentin Antibody (812) [NBP2-43560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [812] diluted at 1:5000.

<b>Genetic Strategies Validation. </b>Knockdown Validated: Vimentin Antibody (812) [NBP2-43560] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812].

Sandwich ELISA: Vimentin Antibody (812) [NBP2-43560] - Sandwich ELISA detection of recombinant full-length Vimentin protein using Vimentin Antibody (812) as capture antibody at concentration of 5 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Fe, Bv, Ca, Ch, Pm, Gp

Species Glossary

Reactivity: Hu, Mu, Rt, Fe, Bv, Ca, Ch, Pm, Gp

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-77067

CXCR4 Antibody - BSA Free

(4 Publications)

0.1 mg

NBP1-77067

Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP

Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NBP1-77067] - HeLa cells with CXCR4 antibody at 2 ug/ml.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NBP1-77067] - Human spleen tissue with CXCR4 antibody at 5 ug/mL.

<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).

Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.

Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.

Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.

Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.

Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NBP1-77067] - HeLa cells with CXCR4 antibody at 20 ug/mL.

Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-125-1

Alt Catalog# IMG-125-1

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77067AF350
Alexa Fluor 405	NBP1-77067AF405
Alexa Fluor 488	NBP1-77067AF488
Alexa Fluor 532	NBP1-77067AF532
Alexa Fluor 594	NBP1-77067AF594
Alexa Fluor 647	NBP1-77067AF647
Alexa Fluor 700	NBP1-77067AF700
Alexa Fluor 750	NBP1-77067AF750
Allophycocyanin	NBP1-77067APC
Biotin	NBP1-77067B
DyLight 350	NBP1-77067UV
DyLight 405	NBP1-77067V
DyLight 488	NBP1-77067G
DyLight 550	NBP1-77067R
DyLight 594	NBP1-77067DL594
DyLight 650	NBP1-77067C
DyLight 680	NBP1-77067FR
DyLight 755	NBP1-77067IR
FITC	NBP1-77067F
HRP	NBP1-77067H
Janelia Fluor 549	NBP1-77067JF549
Janelia Fluor 646	NBP1-77067JF646
PE	NBP1-77067PE
PerCP	NBP1-77067PCP
mFluor Violet 450 SE	NBP1-77067MFV450
mFluor Violet 500 SE	NBP1-77067MFV500
mFluor Violet 610 SE	NBP1-77067MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77067SS

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NBP2-19324

Lamin A + C Antibody

(4 Publications)

0.1 ml

NBP2-19324

Knockout Validated: Lamin A + C Antibody [NBP2-19324] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. The HRP-conjugated anti-rabbit IgG (NBP2-19301) antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19324] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19324] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Lamin A + C stained by Lamin A + C antibody diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mouse stomach. Lamin A + C stained by Lamin A + C antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Lamin A + C Antibody [NBP2-19324] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:1000.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - C2C12 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - RT2 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Human breast carcinoma. Lamin A + C antibody diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mahlarvu xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation: Lamin A + C Antibody [NBP2-19324] - A. 50 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of Lamin A + C protein by 4 ug Lamin A + C antibody (NBP2-19324)7.5 % SDS-PAGE

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Pm, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Pm, RM

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-85814

Vimentin Antibody

(3 Publications)

0.1 ml

NBP1-85814

Knockout Validated: Vimentin Antibody [NBP1-85814] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Rabbit Anti-Human Vimentin Polyclonal Antibody (Catalog # NBP1-85814) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Analysis in human ovary and skeletal muscle tissues. Corresponding VIM RNA-seq data are presented for the same tissues.

Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

<b>Orthogonal Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP1-85814] - Analysis in human cell lines U-251MG and MCF-7 using anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-85814] - Staining of human cell line ASC TERT1 shows localization to intermediate filaments. Antibody staining is shown in green.

Western Blot: Vimentin Antibody [NBP1-85814] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human skeletal muscle shows no positivity in myocytes.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human ovary shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.

Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-29433

Vimentin Antibody (VM452)

(1 Publication)

0.1 mg

NBP2-29433

Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.

<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).

Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).

Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).

Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).

Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Reactivity:

Hu, Po, Bv, Ca, Ch, Fe, Gt, Mu(-), Rt(-)

Species Glossary

Reactivity: Hu, Po, Bv, Ca, Ch, Fe, Gt, Mu(-), Rt(-)

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

NBO-131A

Alt Catalog# NBO-131A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33060AF350
Alexa Fluor 405	NBP2-33060AF405
Alexa Fluor 488	NBP2-33060AF488
Alexa Fluor 532	NBP2-33060AF532
Alexa Fluor 594	NBP2-33060AF594
Alexa Fluor 647	NBP2-33060AF647
Alexa Fluor 700	NBP2-33060AF700
Alexa Fluor 750	NBP2-33060AF750
Allophycocyanin	NBP2-33060APC
Biotin	NBP2-33060B
DyLight 350	NBP2-33060UV
DyLight 405	NBP2-33060V
DyLight 488	NBP2-33060G
DyLight 550	NBP2-33060R
DyLight 594	NBP2-33060DL594
DyLight 650	NBP2-33060C
DyLight 680	NBP2-33060FR
DyLight 755	NBP2-33060IR
FITC	NBP2-33060F
HRP	NBP2-33060H
Janelia Fluor 549	NBP2-33060JF549
Janelia Fluor 646	NBP2-33060JF646
PE	NBP2-33060PE
PerCP	NBP2-33060PCP
mFluor Violet 450 SE	NBP2-33060MFV450
mFluor Violet 500 SE	NBP2-33060MFV500
mFluor Violet 610 SE	NBP2-33060MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33060
IHC-Prediluted	NBP2-44830

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NBP2-19459

N-Cadherin Antibody

(1 Publication)

0.1 ml

NBP2-19459

Knockout Validated: N-Cadherin Antibody [NBP2-19459] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody [NBP2-19459] - N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: N-Cadherin Antibody [NBP2-19459] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: N-Cadherin Antibody [NBP2-19459] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-cadherin antibody ( diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: N-Cadherin Antibody [NBP2-19459] - A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 5% SDS-PAGE N-Cadherin antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: N-Cadherin Antibody [NBP2-19459] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: N-Cadherin Antibody [NBP2-19459] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Human hepatoma. N-Cadherin antibody [N1N3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Rat liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-19457

N-Cadherin Antibody

0.1 ml

NBP2-19457

Knockout Validated: N-Cadherin Antibody [NBP2-19457] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: N-Cadherin Antibody [NBP2-19457] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Frozen: N-Cadherin Antibody [NBP2-19457] - Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: N-Cadherin Antibody [NBP2-19457] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody [NBP2-19457] - SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: N-Cadherin protein stained by N-Cadherin antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19457] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: N-Cadherin Antibody [NBP2-19457] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: N-Cadherin Antibody [NBP2-19457] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Frozen: N-Cadherin Antibody [NBP2-19457] - Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody diluted at 1:500.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-15754

Lamin A + C Antibody (4L8Q0)

100 ul

NBP3-15754

Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts of various cell lines, using Lamin A + C antibody (NBP3-15754) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of U2OS cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A + C antibody (NBP3-15754) at dilution of 1:400 - 1:3200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts of HeLa cells, using Lamin A + C antibody (NBP3-15754) at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts of various cell lines, using Lamin A + C antibody (NBP3-15754) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of H9C2 cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of L929 cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Confocal immunofluorescence analysis of HeLa cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A + C antibody (NBP3-15754) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

<b>Genetic Strategies Validation. </b>Knockout Validated: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts from normal (control) and Lamin A + C knockout (KO) HeLa cells, using Lamin A + C antibody (NBP3-15754) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A + C antibody (NBP3-15754). Western blot was performed from the immunoprecipitate using Lamin A + C antibody (NBP3-15754) at a dilition of 1:20000.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-19323

Lamin A + C Antibody

0.1 ml

NBP2-19323

Knockout Validated: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

<b>Genetic Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19323] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19323] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using Lamin A/C antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:500.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human breast carcinoma. Lamin A + C antibody dilution: 1:500.

Western Blot: Lamin A + C Antibody [NBP2-19323] - A. 30 ug C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Lamin A + C antibody dilution: 1:1000

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Lamin A/C antibody detects LMNA protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Lamin A/C antibody dilution: 1:500.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse prostate. Lamin A + C antibody dilution: 1:500.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse fore brain. Lamin A + C antibody dilution: 1:500.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse skin. Lamin A + C antibody dilution: 1:500.

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human endometrial carcinoma. Lamin A + C antibody dilution: 1:500.

Immunoprecipitation: Lamin A + C Antibody [NBP2-19323] - Immunoprecipitation of Lamin A + C protein from HeLa whole cell extracts using 5 ug of Lamin A + C antibody. Western blot analysis was performed using Lamin A + C antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Reactivity:

Hu, Mu, Po, Ca, Pm, RM

Species Glossary

Reactivity: Hu, Mu, Po, Ca, Pm, RM

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-19460

N-Cadherin Antibody

0.1 ml

NBP2-19460

Knockout Validated: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody [NBP2-19460] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal diluted at 1:1000.

Immunohistochemistry-Frozen: N-Cadherin Antibody [NBP2-19460] - Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal ) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: N-Cadherin Antibody [NBP2-19460] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal diluted at 1:1000.

Immunohistochemistry-Paraffin: N Cadherin Antibody [NBP2-19460] - Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19460] - Paraffin-embedded hepatoma. N-Cadherin antibody [N2C1], Internal dilution: 1:500.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-52867

Vimentin Antibody (CL0157)

0.1 ml

NBP2-52867

Knockout Validated: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human skin shows distinct positivity in fibroblasts.

Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa] Lane 2: Human tonsil tissue lysate

$Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos$

Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human cerebellum shows immunoreactivity in the endothelial cells.

Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human kidney shows strong immunoreactivity in renal glomeruli but not tubuli cells.

Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human prostate shows strong immunoreactivity in the connective tissue.

Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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