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![Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783] - Analysis in human cell line MCF-7.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Western-Blot-NBP2-59783-img0012.jpg "Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783] - Analysis in human cell line MCF-7.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of A-431 cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunocytochemistry-Immunofluorescence-NBP2-59783-img0011.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human colon shows nuclear membrane positivity in glandular and connective tissue cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of MCF7 cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunocytochemistry-Immunofluorescence-NBP2-59783-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of U-251 cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunocytochemistry-Immunofluorescence-NBP2-59783-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of HeLa cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunocytochemistry-Immunofluorescence-NBP2-59783-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of U-2 OS cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunocytochemistry-Immunofluorescence-NBP2-59783-img0010.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human tonsil shows strong nuclear membrane immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human oral mucosa shows nuclear membrane positivity in the epithelial cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human prostate shows nuclear membrane immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human small intestine shows nuclear membrane immunoreactivity in glandular and connective tissue cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0004.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human testis shows strong nuclear membrane positivity in a subset of cells in the seminiferous tubules.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0005.jpg)
![Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Western-Blot-NBP2-67405-img0008.jpg "Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of HepG2 cells labeling Lamin B1 with Rabbit anti-Lamin B1 antibody permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lamin B1 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunocytochemistry-Immunofluorescence-NBP2-67405-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0012.jpg)
![Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: Jurkat cell lysate](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Western-Blot-NBP2-67405-img0006.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon cancer tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human liver tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse colon tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Lamin B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0010.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-SI17-06-Immunohistochemistry-Paraffin-NBP2-67405-img0011.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Analysis in human lymph node and skeletal muscle tissue. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-48966-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Analysis in human lymph node and skeletal muscle tissue. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lamin B1 Antibody [NBP2-48966] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Western-Blot-NBP2-48966-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody [NBP2-48966] - Staining of human cell line MCF7 shows localization to nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48966-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Staining of human skeletal muscle shows moderate to strong positivity in nuclear membrane in myocytes.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-48966-img0008.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Staining of human lymph node shows moderate to strong positivity in nuclear membrane in non-germinal center cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-48966-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Staining of human skin shows moderate to strong positivity in nuclear membrane in keratinocytes.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-48966-img0006.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Staining of human small intestine shows moderate to strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-48966-img0007.jpg)
![Western Blot: Lamin B1 Antibody [NBP3-03711] - Analysis of extracts of various cell lines, using Lamin B1 antibody at 1:1000 dilution.L929 cells were treated by staurosporine(1 uM) for 3 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Western-Blot-NBP3-03711-img0010.jpg "Western Blot: Lamin B1 Antibody [NBP3-03711] - Analysis of extracts of various cell lines, using Lamin B1 antibody at 1:1000 dilution.L929 cells were treated by staurosporine(1 uM) for 3 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody [NBP3-03711] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Lamin B1 Rabbit pAb (NBP3-03711) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03711-img0012.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP3-03711] - Immunohistochemistry of paraffin-embedded mouse brain using [KO] Lamin B1 antibody (NBP3-03711) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP3-03711-img0015.jpg)
![Western Blot: Lamin B1 Antibody [NBP3-03711] - Analysis of extracts of various cell lines, using Lamin B1 antibody at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37c for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Western-Blot-NBP3-03711-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP3-03711] - Immunohistochemistry of paraffin-embedded human breast using [KO] Lamin B1 antibody (NBP3-03711) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP3-03711-img0013.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP3-03711] - Immunohistochemistry of paraffin-embedded rat brain using [KO] Lamin B1 antibody (NBP3-03711) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP3-03711-img0014.jpg)
![Immunoprecipitation: Lamin B1 Antibody [NBP3-03711] - Analysis of 300ug extracts of MCF7 cells using 3ug Lamin B1 antibody. Western blot was performed from the immunoprecipitate using Lamin B1 antibody at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunoprecipitation-NBP3-03711-img0011.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0009.jpg "Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of monoclonal antibody to LMNB1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Immunocytochemistry-Immunofluorescence-H00004001-M01-img0003.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0006.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Jurkat.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0007.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0008.jpg)
![Immunoprecipitation: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Immunoprecipitation-H00004001-M01-img0004.jpg)
![ELISA: Lamin B1 Antibody (4B10) [H00004001-M01] - Detection limit for recombinant GST tagged LMNB1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-ELISA-H00004001-M01-img0010.jpg)
![Western Blot: Lamin B1 Antibody (4E4) [H00004001-M03] - Analysis of LMNB1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4E4-Western-Blot-H00004001-M03-img0006.jpg "Western Blot: Lamin B1 Antibody (4E4) [H00004001-M03] - Analysis of LMNB1 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (4E4) [H00004001-M03] - Analysis of monoclonal antibody to LMNB1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4E4-Immunocytochemistry-Immunofluorescence-H00004001-M03-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (4E4) [H00004001-M03] - Analysis of monoclonal antibody to LMNB1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4E4-Immunohistochemistry-Paraffin-H00004001-M03-img0003.jpg)
![Western Blot: Lamin B1 Antibody (4E4) [H00004001-M03] - LMNB1 monoclonal antibody (M03), clone 4E4. Western Blot analysis of LMNB1 expression in Jurkat ( Cat # L017V1 ).](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4E4-Western-Blot-H00004001-M03-img0001.jpg)
![Western Blot: Lamin B1 Antibody (4E4) [H00004001-M03] - Analysis of LMNB1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4E4-Western-Blot-H00004001-M03-img0005.jpg)
![ELISA: Lamin B1 Antibody (4E4) [H00004001-M03] - Lamin B1 Antibody (4E4) [H00004001-M03] - Detection limit for recombinant GST tagged LMNB1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4E4-ELISA-H00004001-M03-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (919022) [Unconjugated] [MAB85251] - Lamin B1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB85251) at 1.7 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-919022-Unconjugated-Immunohistochemistry-Paraffin-MAB85251-img0001.jpg "Immunohistochemistry-Paraffin: Lamin B1 Antibody (919022) [Unconjugated] [MAB85251] - Lamin B1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB85251) at 1.7 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei.")
![Western Blot: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Western blot analysis of extracts of various cell lines, using Lamin B1 Rabbit mAb (NBP3-15393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Western-Blot-NBP3-15393-img0004.jpg "Western Blot: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Western blot analysis of extracts of various cell lines, using Lamin B1 Rabbit mAb (NBP3-15393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded mouse testis using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Immunohistochemistry-Paraffin-NBP3-15393-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded rat spleen using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Immunohistochemistry-Paraffin-NBP3-15393-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded human colon using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Immunohistochemistry-Paraffin-NBP3-15393-img0002.jpg)
![Recombinant Human Lamin B1 Protein [H00004001-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004001-P01-1.jpg "Recombinant Human Lamin B1 Protein [H00004001-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human Lamin B1 Protein [H00004001-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004001-Q01-1.jpg "SDS-Page: Recombinant Human Lamin B1 Protein [H00004001-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")