Hu,
Mu,
Rt,
I,
Xp,
Ye,
Ce,
Ch,
Dr,
PlSpecies Glossary
Reactivity: Hu, Mu, Rt, I, Xp, Ye, Ce, Ch, Dr, Pl
WB, ICC/IF, IHC, WB, ChIP, Single-Cell Western
Applications: WB, ICC/IF, IHC, WB, ChIP, Single-Cell Western
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Immunocytochemistry/Immunofluorescence: Histone H3.3A Antibody [NBP1-33767] - Paraformaldehyde-fixed HeLa, using Histone H3. 3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33767-img0006.jpg "Immunocytochemistry/Immunofluorescence: Histone H3.3A Antibody [NBP1-33767] - Paraformaldehyde-fixed HeLa, using Histone H3. 3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.")
![Western Blot: Histone H3.3A Antibody [NBP1-33767] - Sample (30 ug of whole cell lysate) A: IMR32 15% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Western-Blot-NBP1-33767-img0005.jpg)
![Western Blot: Histone H3.3A Antibody [NBP1-33767] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3A antibody (NBP1-33767) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp1-33767_rabbit-polyclonal-histone-h3-3a-antibody-910202420122092.jpg)
![Western Blot: Histone H3.3A Antibody [NBP2-98769] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 16 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Western-Blot-NBP2-98769-img0002.jpg "Western Blot: Histone H3.3A Antibody [NBP2-98769] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 16 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Histone H3.3A Antibody [NBP2-98769] - Histone H3.3A was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate 2 ul anti-Histone H3.3A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Histone H3.3A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Immunoprecipitation-NBP2-98769-img0001.jpg)
![Western Blot: Histone H3.3A Antibody [NBP2-84066] - Host: Rabbit. Target Name: H3F3A. Sample Type: 293T Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Western-Blot-NBP2-84066-img0001.jpg "Western Blot: Histone H3.3A Antibody [NBP2-84066] - Host: Rabbit. Target Name: H3F3A. Sample Type: 293T Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: Histone H3.3A Overexpression Lysate (Adult Normal) [NBL1-11428] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H3.3A.](http://images.novusbio.com/fullsize/Histone-H3-3A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11428-img0002.jpg "Western Blot: Histone H3.3A Overexpression Lysate (Adult Normal) [NBL1-11428] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H3.3A.")
![Western Blot: Histone H3.3A Antibody [NBP2-98768] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: C6 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Western-Blot-NBP2-98768-img0001.jpg "Western Blot: Histone H3.3A Antibody [NBP2-98768] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: C6 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa")
![SDS-Page: Histone H3.3A Protein [NBP2-23098]](http://images.novusbio.com/fullsize/Histone-H3.3A-Protein-SDS-Page-NBP2-23098-img0001.jpg "SDS-Page: Histone H3.3A Protein [NBP2-23098]")
![Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0013.jpg "Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 Antibody [NB500-171] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NB500-171 and 25 uL of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody (No Ab) was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive, and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Chromatin-Immunoprecipitation-NB500-171-img0016.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse testis.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0017.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - MDA-MB-231 cells were treated with vehicle (V) or paclitaxel (P). Cytosolic and nuclear lysates were prepared, and immunoblot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0020.jpg)
![Immunohistochemistry: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-NB500-171-img0012.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, and in vivo from aged donors. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a and b) and adult donors. Anti- used at 1:20,000 and Anti-Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] (NBP2-16848) used at 1:10,000. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1038/s41598-020-59163-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-171 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-171 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - Immunofluorescence staining to detect histone H3 in human blood. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0023.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 antibody on Mouse pancreas tissue. Histone H3 antibody dilution 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0025.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - (1) HeLa, (2) S. cerevisiae whole cell lysates, (3) Histones purified from HeLa cells. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0014.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Total histone H3 levels in MCF-7 and HCT-116 cells. Observed molecular weight is ~17 kDa. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0019.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat pancreas.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0009.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat colon.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse brain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0007.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - p53 but not ATM is required for chronic gamma -radiation-induced histone reductions. (a) Western blots of histone proteins in wild-type, TP53−/− & ATM−/− RPE-1 cells following exposures to 20 mGy/h chronic radiation for 7 days. (b) ATM activation, as shown by auto-phosphorylation on S1981, in response to chronic radiation in primary fibroblasts. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-31020241539715.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma -radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-31020241534368.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma -radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-310202415291940.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Chronic gamma -radiation reduces histone levels. (a) Western blot analyses of histone H2AX & gamma H2AX in primary fibroblasts exposed to various dose-rates of chronic gamma -radiation for 7 days. Cells irradiated with a single acute dose of 4 Gy X-ray were included as control. (b) Effect of chronic gamma -irradiation on H2AX levels in three different isogenic primary cell types from a different donor to that used in (a) at the same dose rates as in (a). (c) Immunoblots of other histones in chronically irradiated primary fibroblasts. (d) All significant histone level changes detected by SILAC LC-MS/MS protein analyses of samples from primary fibroblasts exposed or mock-exposed to chronic gamma -radiation for 7 days. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-310202415392548.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0012.jpg "Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.")
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunohistochemistry-Paraffin-NBP1-30141-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0011.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 (NBP1-30141) using HeLa nuclear lysate [NB800-PC9]. Observed molecular weight ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of A549 cells (fixed with methanol) using Histone H3 [Trimethyl Lys9] antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0014.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Used to perform ChIP on HeLa cells.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Chromatin-Immunoprecipitation-NBP1-30141-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - H3K9Me3 analysis of MCF7 and HCT116. Theorectical molecular weight is ~15 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0008.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0013.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0004.jpg)
![Histone H3 [Trimethyl Lys9] (6F12-H4) was detected in immersion fixed MCF7 human breast cancer cell line using Mouse anti- Histone H3 [Trimethyl Lys9] (6F12-H4) Protein G Purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-30141R) (red) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/antibody/nbp1-30141_mouse-monoclonal-histone-h3-trimethyl-lys9-antibody-6f12-h4-immunocytochemistry-immunofluorescence-2382024134224..jpg)