| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26046 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9448-SP | |||
| Carrier Free | IC9448V-100UG | |||
| Carrier Free | IC9448T-100UG | |||
| Carrier Free | IC9448S-100UG | |||
| Carrier Free | IC9448R-100UG | |||
| Carrier Free | IC9448N-100UG | |||
| Carrier Free | IC9448G-100UG | |||
| Carrier Free | IC9448U-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52482-0.025mg | |||
![Western Blot: Histone H3.3 Antibody [NBP2-24697] - Total protein from human HeLa and A431 cells and mouse Neuro2A cells was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Histone H3.3 in blocking buffer with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Western-Blot-NBP2-24697-img0003.jpg "Western Blot: Histone H3.3 Antibody [NBP2-24697] - Total protein from human HeLa and A431 cells and mouse Neuro2A cells was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Histone H3.3 in blocking buffer with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697] - Human small intestine stained with Histone H3.3 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Immunohistochemistry-Paraffin-NBP2-24697-img0002.jpg)
![Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Western blot analysis of Histone H3.3 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Western-Blot-NBP2-67530-img0008.jpg "Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Western blot analysis of Histone H3.3 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunocytochemistry-Immunofluorescence-NBP2-67530-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0014.jpg)
![Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of Histone H3.3 on BT-20 cell lysates using anti-Histone H3.3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Western-Blot-NBP2-67530-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunocytochemistry-Immunofluorescence-NBP2-67530-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse brain tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse stomach tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0009.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human lung tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0011.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human cervix tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0012.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0013.jpg)
![Western Blot: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Western blot analysis of extracts of various cell lines, using Histone H3.3 Rabbit mAb (NBP3-16489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Western-Blot-NBP3-16489-img0005.jpg "Western Blot: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Western blot analysis of extracts of various cell lines, using Histone H3.3 Rabbit mAb (NBP3-16489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Immunohistochemistry of paraffin-embedded mouse liver using Histone H3.3 Rabbit mAb (NBP3-16489) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Immunohistochemistry-Paraffin-NBP3-16489-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H3.3 antibody (NBP3-16489) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Chromatin-Immunoprecipitation-NBP3-16489-img0006.jpg)
![Western Blot: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Western blot analysis of extracts of Rat lung, using Histone H3.3 Rabbit mAb (NBP3-16489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Western-Blot-NBP3-16489-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Immunohistochemistry of paraffin-embedded rat kidney using Histone H3.3 Rabbit mAb (NBP3-16489) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Immunohistochemistry-Paraffin-NBP3-16489-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Immunohistochemistry of paraffin-embedded human esophageal using Histone H3.3 Rabbit mAb (NBP3-16489) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Immunohistochemistry-Paraffin-NBP3-16489-img0002.jpg)
![Western Blot: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: Histone H3.3 [p Thr3] Antibody at 1.41 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa Observed band size: 16 KDa](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-282202411472125.jpg "Western Blot: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: Histone H3.3 [p Thr3] Antibody at 1.41 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa Observed band size: 16 KDa")
![Immunocytochemistry/Immunofluorescence: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Analysis of Histone H3.3 [p Thr3] Antibody (28H4) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-28220241323624.jpg)
![Flow Cytometry: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Overlay histogram showing Hela cells stained with Histone H3.3 [p Thr3] Antibody (28H4) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-282202414183663.jpg)
![Immunohistochemistry: Histone H3.3 Antibody (4H2) [NBP3-26227] - Image of Histone H3.3 Antibody (4H2) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26227_rabbit-histone-h3-3-mab-4h2-262202415475637.jpg "Immunohistochemistry: Histone H3.3 Antibody (4H2) [NBP3-26227] - Image of Histone H3.3 Antibody (4H2) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: Histone H3.3 Antibody (4H2) [NBP3-26227] - Image of Histone H3.3 Antibody (4H2) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26227_rabbit-histone-h3-3-mab-4h2-27220248203911.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (4H2) [NBP3-26227] - Staining of Hela cells with Histone H3.3 Antibody (4H2) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26227_rabbit-histone-h3-3-mab-4h2-282202412493619.jpg)
![Western Blot: Histone H3.3 Antibody (S04-5J3) [NBP3-14894] - Western blot detection of Histone H3. 3 in K562, C6, 3T3 cell lysates using Histone H3. 3 Rabbit mAb (1:1000 diluted). Predicted band size: 15kDa. Observed band size: 15kDa.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-S04-5J3-Western-Blot-NBP3-14894-img0001.jpg "Western Blot: Histone H3.3 Antibody (S04-5J3) [NBP3-14894] - Western blot detection of Histone H3. 3 in K562, C6, 3T3 cell lysates using Histone H3. 3 Rabbit mAb (1:1000 diluted). Predicted band size: 15kDa. Observed band size: 15kDa.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (S04-5J3) [NBP3-14894] - Immunohistochemistry of Histone H3. 3 in paraffin-embedded Human lung cancer tissue using Histone H3. 3 Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-S04-5J3-Immunohistochemistry-Paraffin-NBP3-14894-img0002.jpg)
![Western Blot: Histone H3.3 [p Ser 31] Antibody (SR1217) [NBP3-21599] - Western blot analysis using NBP3-21599 in HeLa cell treated with Calyculin A lysate.](http://images.novusbio.com/fullsize/nbp3-21599_rabbit-histone-h3-3-p-ser-31-mab-sr1217-57202317215133.jpg "Western Blot: Histone H3.3 [p Ser 31] Antibody (SR1217) [NBP3-21599] - Western blot analysis using NBP3-21599 in HeLa cell treated with Calyculin A lysate.")
![Western Blot: Histone H3.3 Antibody (190) [NBP2-61548] - HeLa whole cell lysate, recombinant Histone H3.3 and Histone H3.1 proteins, using 190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-190-Western-Blot-NBP2-61548-img0003.jpg "Western Blot: Histone H3.3 Antibody (190) [NBP2-61548] - HeLa whole cell lysate, recombinant Histone H3.3 and Histone H3.1 proteins, using 190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.")
![ELISA: Histone H3.3 Antibody (190) [NBP2-61548] - Reacts specifically to Histone H3.3. No cross reactivity with Histone H3.1.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-190-ELISA-NBP2-61548-img0001.jpg)
![Western Blot: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Analysis using AHH3 mouse mAb against NIH3T3 (1), Hela (2), K562 (3) cell lysate.](http://images.novusbio.com/fullsize/HIST3H3-Antibody-4E9B11-Western-Blot-NBP2-52482-img0003.jpg "Western Blot: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Analysis using AHH3 mouse mAb against NIH3T3 (1), Hela (2), K562 (3) cell lysate.")
![Flow Cytometry: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Analysis of NIH/3T3 cells using HH3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/HIST3H3-Antibody-4E9B11-Flow-Cytometry-NBP2-52482-img0001.jpg)
![ELISA: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/HIST3H3-Antibody-4E9B11-ELISA-NBP2-52482-img0002.jpg)
![Western Blot: ZC3H3 Antibody [NBP1-03341] - Detection of Human ZC3H3 on HeLa whole cell lysate using NBP1-03341. ZC3H3 was also immunoprecipitated by another rabbit anti-ZC3H3 antibody.](http://images.novusbio.com/fullsize/ZC3H3-Antibody-Western-Blot-NBP1-03341-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ZC3H3 Antibody [NBP1-03341] - Detection of Human ZC3H3 on HeLa whole cell lysate using NBP1-03341. ZC3H3 was also immunoprecipitated by another rabbit anti-ZC3H3 antibody.")
![Immunohistochemistry-Paraffin: ZC3H3 Antibody [NBP1-81536] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ZC3H3-Antibody-Immunohistochemistry-Paraffin-NBP1-81536-img0002.jpg "Immunohistochemistry-Paraffin: ZC3H3 Antibody [NBP1-81536] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.")