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![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0011.jpg "Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F9NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B in human prostate](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0010.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0007.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis of S100B (15F4BN) in human brain tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in human prostate tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B (15F9NB) in human brain.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0009.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) [Azide Free] [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0002.jpg)
![Western Blot: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of MYH9 expression in transfected 293T cell line by MYH9 monoclonal antibody (M03), clone 2B3. Lane 1: MYH9 transfected lysate (Predicted MW: 10.01 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Western-Blot-H00004627-M03-img0006.jpg "Western Blot: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of MYH9 expression in transfected 293T cell line by MYH9 monoclonal antibody (M03), clone 2B3. Lane 1: MYH9 transfected lysate (Predicted MW: 10.01 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Bovine ovarian granulosa cells. Anti Myosin IIA immunofluorescent staining (green). DNA in counterstained with DAPI (blue). Control was obtained by omitting the primary antibody. Fixation: 4% PF for 7 minutes at rt. Permeabilization: 0.1% Triton-X in PBS for 7 minutes. Blocking: 20% donkey serum, 1% BSA in PBS, 1 hour at rt. Primary antibody: 1:50 or 1:100 in 1% BSA/PBS over night at 4C. Secondary antibody: donkey anti mouse -Alexa 488 (Invitrogen), 1:500. DNA Counterstaining: mounting medium with DAPI. Negative CTRL was performed by omitting the primary antibody, images were captured under an epifluorescence microscope. Image from verified customer review.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Immunocytochemistry-Immunofluorescence-H00004627-M03-img0007.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of monoclonal antibody to MYH9 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 0.5 ug/ml](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Immunohistochemistry-Paraffin-H00004627-M03-img0003.jpg)
![Western Blot: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of MYH9 expression in HeLa.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Western-Blot-H00004627-M03-img0004.jpg)
![Western Blot: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of MYH9 expression in human kidney.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Western-Blot-H00004627-M03-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of monoclonal antibody to MYH9 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-Immunocytochemistry-Immunofluorescence-H00004627-M03-img0002.jpg)
![ELISA: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Detection limit for recombinant GST tagged MYH9 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-2B3-ELISA-H00004627-M03-img0001.jpg)
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded cow small intestine stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0007.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded cow small intestine stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded human skeletal muscle stained with Desmin antibody (DES/3255) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0001.jpg)
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded human prostate stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0002.jpg)
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded human cardiac muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0003.jpg)
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded cat smooth muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0004.jpg)
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded dog smooth muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0005.jpg)
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/3255) [NBP3-13866] - Formalin-fixed, paraffin-embedded horse smooth muscle stained with Desmin antibody (DES/3255) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-3255-Immunohistochemistry-Paraffin-NBP3-13866-img0006.jpg)
![Flow Cytometry: HLA DRA Antibody (169-1B5.2) [NBP2-45043] - Flow Cytometric Analysis of PFA-fixed Raji cells. HLA DRA Antibody (169-1B5.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-169-1B5-2-Flow-Cytometry-NBP2-45043-img0004.jpg "Flow Cytometry: HLA DRA Antibody (169-1B5.2) [NBP2-45043] - Flow Cytometric Analysis of PFA-fixed Raji cells. HLA DRA Antibody (169-1B5.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")