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![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0005.jpg "Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A")
![Immunohistochemistry-Paraffin: nNOS Antibody [NB100-858] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NB100-858-img0006.jpg)
![Flow Cytometry: nNOS Antibody [NB100-858] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nNOS-Antibody-Flow-Cytometry-NB100-858-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Wholemount preparations of the myenteric neurons in the distal colon segments following 3 (2a-2d) and 7 (4a-4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 3 (1a-1d) and 7 (3a-3d) days. Myenteric ganglia and neurons labeled with beta-Tubulin Tuj1 (red), nuclei labeled with DAPI (blue) (arrows) can be seen within the ganglion (1b). Smooth muscle cells (asterisks) are also labeled with DAPI (1b). Significant increase in the proportion of nNOS neurons (green) can be seen at both Days 3 and 7 after oxaliplatin treatment. Scale bar = 20 um. Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/article/10.3389/fnins.2013.00030/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0004.jpg)
![Western Blot: nNOS Antibody [NBP1-39681] - Effects of ER alpha and ER beta Antagonists on NE Regulation of VMN nNOS Protein Expression. Micropunch-dissected VMN tissue was obtained from groups of ovariectomized, estradiol-replaced female rats pretreated by intra-VMN delivery of MPP, PHTPP, or vehicle (Veh) prior to NE administration for Western blot analysis of neuronal nitric oxide synthase (nNOS) - Panel 2A,F(5, 12)=12.67,p<.0001 - protein expression. Data depict mean normalized protein optical density (O.D.) values+/-SEMfor groups of rats (n=6 per group) infused with Veh (white bars) or NE (gray bars) after delivery of Veh (solid bars), MPP (diagonal-striped bars), or PHTPP (cross-hatched bars) administration. *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nNOS-Antibody-Western-Blot-NBP1-39681-img0007.jpg "Western Blot: nNOS Antibody [NBP1-39681] - Effects of ER alpha and ER beta Antagonists on NE Regulation of VMN nNOS Protein Expression. Micropunch-dissected VMN tissue was obtained from groups of ovariectomized, estradiol-replaced female rats pretreated by intra-VMN delivery of MPP, PHTPP, or vehicle (Veh) prior to NE administration for Western blot analysis of neuronal nitric oxide synthase (nNOS) - Panel 2A,F(5, 12)=12.67,p<.0001 - protein expression. Data depict mean normalized protein optical density (O.D.) values+/-SEMfor groups of rats (n=6 per group) infused with Veh (white bars) or NE (gray bars) after delivery of Veh (solid bars), MPP (diagonal-striped bars), or PHTPP (cross-hatched bars) administration. *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-39681] - Analysis of anti-nNOS / NOS1 antibody with human brain, cerebellum at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NBP1-39681-img0004.jpg)
![Western Blot: nNOS Antibody [NBP1-39681]](http://images.novusbio.com/fullsize/nNOS-Antibody-Western-Blot-NBP1-39681-img0006.jpg)
![Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-39681] - Analysis of anti-nNOS / NOS1 antibody with human brain, cortex at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NBP1-39681-img0003.jpg)
![Western Blot: nNOS Antibody (5K9R2) [NBP3-16008] - Western blot analysis of extracts of Rat brain, using nNOSantibody (NBP3-16008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/nNOS-Antibody-5K9R2-Western-Blot-NBP3-16008-img0004.jpg "Western Blot: nNOS Antibody (5K9R2) [NBP3-16008] - Western blot analysis of extracts of Rat brain, using nNOSantibody (NBP3-16008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (5K9R2) [NBP3-16008] - Immunofluorescence analysis of SH-SY5Y cells using nNOSRabbit mAb (NBP3-16008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nNOS-Antibody-5K9R2-Immunocytochemistry-Immunofluorescence-NBP3-16008-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (5K9R2) [NBP3-16008] - Immunofluorescence analysis of Neuro-2a cells using nNOSRabbit mAb (NBP3-16008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nNOS-Antibody-5K9R2-Immunocytochemistry-Immunofluorescence-NBP3-16008-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (5K9R2) [NBP3-16008] - Immunofluorescence analysis of PC-12 cells using nNOSRabbit mAb (NBP3-16008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nNOS-Antibody-5K9R2-Immunocytochemistry-Immunofluorescence-NBP3-16008-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (M401) [NBP3-23224] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23224_mouse-nnos-mab-m401-19920231856948.jpg "Immunocytochemistry/Immunofluorescence: nNOS Antibody (M401) [NBP3-23224] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: nNOS Antibody (M401) [NBP3-23224] - Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (NBP3-23224) (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (NBP3-23225) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23224_mouse-nnos-mab-m401-19920231820296.jpg)
![Western Blot: nNOS Antibody [NBP3-23225] - Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (NBP3-23224) (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (NBP3-23225) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23225_rabbit-nnos-pab-1992023185693.jpg "Western Blot: nNOS Antibody [NBP3-23225] - Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (NBP3-23224) (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (NBP3-23225) (lanes 3 & 4).")
![SDS-Page: Recombinant Human nNOS Protein [H00004842-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004842-Q01-1.jpg "SDS-Page: Recombinant Human nNOS Protein [H00004842-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: nNOS Overexpression Lysate (Adult Normal) [NBL1-13719] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for nNOS.](http://images.novusbio.com/fullsize/nNOS-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13719-img0002.jpg "Western Blot: nNOS Overexpression Lysate (Adult Normal) [NBL1-13719] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for nNOS.")
![ELISA: Mouse nNOS ELISA Kit (Colorimetric) [NBP2-80253] - Samples were spiked with high concentrations of Mouse nNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80253_mouse-nnos-elisa-kit-colorimetric-132202416254023.jpg "ELISA: Mouse nNOS ELISA Kit (Colorimetric) [NBP2-80253] - Samples were spiked with high concentrations of Mouse nNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse nNOS ELISA Kit (Colorimetric) [NBP2-80253] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80253_mouse-nnos-elisa-kit-colorimetric-432024857486.jpg)
![ELISA: Rat nNOS ELISA Kit (Colorimetric) [NBP2-80254] - Samples were spiked with high concentrations of Rat nNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80254_rat-nnos-elisa-kit-colorimetric-132202416133614.jpg "ELISA: Rat nNOS ELISA Kit (Colorimetric) [NBP2-80254] - Samples were spiked with high concentrations of Rat nNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat nNOS ELISA Kit (Colorimetric) [NBP2-80254] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80254_rat-nnos-elisa-kit-colorimetric-4320249163360.jpg)
![ELISA: Human nNOS ELISA Kit (Colorimetric) [NBP2-80252] - Samples were spiked with high concentrations of Human nNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80252_human-nnos-elisa-kit-colorimetric-13220241620481.jpg "ELISA: Human nNOS ELISA Kit (Colorimetric) [NBP2-80252] - Samples were spiked with high concentrations of Human nNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human nNOS ELISA Kit (Colorimetric) [NBP2-80252] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80252_human-nnos-elisa-kit-colorimetric-4320249141691.jpg)