Hu,
Mu,
Po,
Am,
Bv,
Ca,
Ch,
ChHa,
Eq,
Fe,
Ha,
Pm,
Rb,
Rt,
Av,
Pm,
Pm,
Pm-Or,
ReSpecies Glossary
Reactivity: Hu, Mu, Po, Am, Bv, Ca, Ch, ChHa, Eq, Fe, Ha, Pm, Rb, Rt, Av, Pm, Pm, Pm-Or, Re
WB, Simple Western
Applications: WB, Simple Western
Host: Rabbit Polyclonal
Alt Catalog# IMG-6803A
Sample Size Available
Sizes: Sample Size Available
![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein level in different brain regions. Confocal images from the hippocampus and basolateral amygdala of control and ESI mice reacted with SIRT1 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunohistochemistry-NBP1-51641-img0015.jpg "Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein level in different brain regions. Confocal images from the hippocampus and basolateral amygdala of control and ESI mice reacted with SIRT1 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125) licensed under a CC-BY license.")
![Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Western-Blot-NBP1-51641-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0012.jpg)
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Cytometry-NBP1-51641-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunohistochemistry-Paraffin-NBP1-51641-img0003.jpg)
![Flow (Intracellular): Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - An intracellular stain was performed on RAW 246.7 cells with Sirtuin 1/SIRT1 Antibody (1F3) NBP1-51641APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Intracellular-NBP1-51641-img0014.jpg)
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Cytometry-NBP1-51641-img0005.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Western-Blot-NBP2-27205-img0003.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Simple Western lane view shows a specific band for Sirtuin 1/SIRT1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Simple-Western-NBP2-27205-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87038-img0004.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0009.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Saining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0007.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0008.jpg)
![Sirtuin 1/SIRT1 Assay Kit (Fluorometric) [KA1366] - Inhibition of SIRT1 by Sirtinol (IC50= 38 uM).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Assay-Kit-Fluorometric-KA1366-img0001.jpg "Sirtuin 1/SIRT1 Assay Kit (Fluorometric) [KA1366] - Inhibition of SIRT1 by Sirtinol (IC50= 38 uM).")
![Western Blot: Sirtuin 1/SIRT1 Antibody (S06-4K9) [NBP3-14881] - Western blot detection of Sirtuin 1/SIRT1 in K562, C6, 3T3, Hela cell lysates using Sirtuin 1/SIRT1 Rabbit mAb (1:1000 diluted). Predicted band size: 82kDa. Observed band size: 120kDa.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-S06-4K9-Western-Blot-NBP3-14881-img0001.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody (S06-4K9) [NBP3-14881] - Western blot detection of Sirtuin 1/SIRT1 in K562, C6, 3T3, Hela cell lysates using Sirtuin 1/SIRT1 Rabbit mAb (1:1000 diluted). Predicted band size: 82kDa. Observed band size: 120kDa.")
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP2-56849] - Staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56849-img0001.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP2-56849] - Staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.")
![ELISA: Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric) [NBP2-80301] - Samples were spiked with high concentrations of Mouse Sirtuin 1/SIRT1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80301_mouse-sirtuin-1-sirt1-elisa-kit-colorimetric-132202416184887.jpg "ELISA: Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric) [NBP2-80301] - Samples were spiked with high concentrations of Mouse Sirtuin 1/SIRT1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric) [NBP2-80301] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80301_mouse-sirtuin-1-sirt1-elisa-kit-colorimetric-4320249124821.jpg)