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![Western Blot: ITCH/AIP4 Antibody [NB100-68142] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Western-Blot-NB100-68142-img0002.jpg "Western Blot: ITCH/AIP4 Antibody [NB100-68142] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Flow Cytometry: ITCH/AIP4 Antibody [NB100-68142] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Flow-Cytometry-NB100-68142-img0005.jpg)
![Immunofluorescence: ITCH/AIP4 Antibody [NB100-68142] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Immunofluorescence-NB100-68142-img0003.jpg)
![Immunofluorescence: ITCH/AIP4 Antibody [NB100-68142] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Immunofluorescence-NB100-68142-img0004.jpg)
![Western Blot: ITCH/AIP4 Antibody [NBP2-55083] - Analysis in human cell lines Caco-2 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Western-Blot-NBP2-55083-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ITCH/AIP4 Antibody [NBP2-55083] - Analysis in human cell lines Caco-2 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ITCH/AIP4 Antibody [NBP2-55083] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55083-img0001.jpg)
![Western Blot: ITCH/AIP4 Antibody [NBP2-55083] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Western-Blot-NBP2-55083-img0002.jpg)
![Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts of various cell lines, using ITCH/AIP4 Rabbit mAb (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-7I7B10-Western-Blot-NBP3-16669-img0002.jpg "Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts of various cell lines, using ITCH/AIP4 Rabbit mAb (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts of various cell lines, using ITCH/AIP4 Rabbit mAb (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-7I7B10-Western-Blot-NBP3-16669-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts from normal (control) and ITCH/AIP4 knockout (KO) 293T cells, using ITCH/AIP4 antibody (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-7I7B10-Knockout-Validated-NBP3-16669-img0001.jpg)
![Immunohistochemistry-Paraffin: ITCH/AIP4 Antibody [NBP1-89180] - Staining of human duodenum shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Immunohistochemistry-Paraffin-NBP1-89180-img0002.jpg "Immunohistochemistry-Paraffin: ITCH/AIP4 Antibody [NBP1-89180] - Staining of human duodenum shows strong positivity in glandular cells.")
![Immunocytochemistry/Immunofluorescence: ITCH/AIP4 Antibody (01) [NBP3-06616] - Staining of Human ITCH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ITCH monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-01-Immunocytochemistry-Immunofluorescence-NBP3-06616-img0001.jpg "Immunocytochemistry/Immunofluorescence: ITCH/AIP4 Antibody (01) [NBP3-06616] - Staining of Human ITCH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ITCH monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Flow Cytometry: ITCH/AIP4 Antibody (01) [NBP3-06616] - Analysis of Human ITCH expression on HeLa cells. The cells were treated, stained with purified anti-Human ITCH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-01-Flow-Cytometry-NBP3-06616-img0002.jpg)
![Western Blot: ITCH/AIP4 Antibody [NBP2-81837] - Western blot analysis of ITCH/AIP4 in 3T3 cell lysate with ITCH/AIP4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Western-Blot-NBP2-81837-img0001.jpg "Western Blot: ITCH/AIP4 Antibody [NBP2-81837] - Western blot analysis of ITCH/AIP4 in 3T3 cell lysate with ITCH/AIP4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: ITCH/AIP4 Antibody (1B8) [H00083737-M06] - Analysis of monoclonal antibody to ITCH on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-1B8-Immunocytochemistry-Immunofluorescence-H00083737-M06-img0001.jpg "Immunocytochemistry/Immunofluorescence: ITCH/AIP4 Antibody (1B8) [H00083737-M06] - Analysis of monoclonal antibody to ITCH on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: ITCH/AIP4 Antibody (1B8) [H00083737-M06] - Detection limit for recombinant GST tagged ITCH is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-1B8-Sandwich-ELISA-H00083737-M06-img0002.jpg)
![Western Blot: ITCH/AIP4 Antibody (S03-4I6) [NBP3-19881] - Western blot detection of ITCH/AIP4 in A549, HL-60 cell lysates using NBP3-19881 (1:1000 diluted). Predicted band size: 103kDa. Observed band size: 103kDa.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-S03-4I6-Western-Blot-NBP3-19881-img0001.jpg "Western Blot: ITCH/AIP4 Antibody (S03-4I6) [NBP3-19881] - Western blot detection of ITCH/AIP4 in A549, HL-60 cell lysates using NBP3-19881 (1:1000 diluted). Predicted band size: 103kDa. Observed band size: 103kDa.")
![Western Blot: ITCH/AIP4 Antibody [NBP1-18881] - Whole cell lysate (5, 15 and 50 ug) from Jurkat cells. Antibody used at 0.04 ug/ml.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Western-Blot-NBP1-18881-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: ITCH/AIP4 Antibody [NBP1-18881] - Whole cell lysate (5, 15 and 50 ug) from Jurkat cells. Antibody used at 0.04 ug/ml.")
![Western Blot: ITCH Overexpression Lysate (Adult Normal) [NBL1-12057] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ITCH.](http://images.novusbio.com/fullsize/ITCH-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12057-img0002.jpg "Western Blot: ITCH Overexpression Lysate (Adult Normal) [NBL1-12057] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ITCH.")
![Western Blot: ITCH/AIP4 Antibody (9C3) [NBP3-15151] - Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate, THP-1 whole cell lysate. All lanes: ITCH/AIP4 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 103, 99, 87 KDa. Observed band size: 103 KDa.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-9C3-Western-Blot-NBP3-15151-img0001.jpg "Western Blot: ITCH/AIP4 Antibody (9C3) [NBP3-15151] - Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate, THP-1 whole cell lysate. All lanes: ITCH/AIP4 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 103, 99, 87 KDa. Observed band size: 103 KDa.")