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![Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha tubulin in Hela cell line. Cells, cultured on cover slips, were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 1:200 dilution of anti-58K Golgi Protein antibody clone 58K-9 for overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) and the cells were then imaged using a 40X objective.](http://images.novusbio.com/fullsize/DAPI-Solution-Immunofluorescence-NBP2-31156-img0002.jpg "Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha tubulin in Hela cell line. Cells, cultured on cover slips, were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 1:200 dilution of anti-58K Golgi Protein antibody clone 58K-9 for overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) and the cells were then imaged using a 40X objective.")
![Immunohistochemistry: DAPI Solution [NBP2-31156] - DAPI labelling in mouse brain. Image from verified customer review.](http://images.novusbio.com/fullsize/DAPI-Solution-Immunohistochemistry-NBP2-31156-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - ICC analysis of LM7 cells using anti-IgM antibody [DyLight 550] (Cat.# NBP2-34422R). (blue) was used as a nuclear counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/DAPI-Solution-Immunocytochemistry-Immunofluorescence-NBP2-31156-img0001.jpg)
![In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag Biotin-labeled pUC1.77 detected with Texas Red Avidin DCS, Biotinylated Anti-Avidin and Texas Red Avidin DCS, and counterstained with VECTASHIELD with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Antifade-Mounting-Medium-with-DAPI-In-situ-Hybridization-H-1200-NB-img0006.jpg "In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag Biotin-labeled pUC1.77 detected with Texas Red Avidin DCS, Biotinylated Anti-Avidin and Texas Red Avidin DCS, and counterstained with VECTASHIELD with DAPI.")
![In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red Substrate. Mounted in VECTASHIELD Mounting Media with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Antifade-Mounting-Medium-with-DAPI-In-situ-Hybridization-H-1200-NB-img0007.jpg)
![In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Antifade-Mounting-Medium-with-DAPI-In-situ-Hybridization-H-1200-NB-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0060.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Medium](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0067.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0041.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0042.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0043.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0044.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 594 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0046.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 594 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0047.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 549 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0048.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 488 labeled secondary antibody (green). S100, DyLight 594 labeled secondary antibody (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0049.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0051.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0052.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0053.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0054.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0055.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0056.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0057.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0058.jpg)
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 549 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0059.jpg)
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Texas Red Avidin DCS (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0061.jpg)
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Medium](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0062.jpg)
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Mediu](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0063.jpg)
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Medium](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0064.jpg)
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Retina from mice, perfused with DyLight 594-LEL. The retina were sectioned and mounted in Vectashield HardSet+DAPI. Image courtesy of George W. Smith, Florida Atlantic University](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0065.jpg)
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Texas Red Avidin DCS (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0066.jpg)
![Cellular Imaging: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 488 labeled secondary antibody (green). S100, DyLight 594 labeled secondary antibody (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Cellular-Imaging-H-1500-NB-img0050.jpg)
![VECTASHIELD® Vibrance(TM) Antifade Mounting Medium with DAPI [H-1800-10-NB] - Human FFPE Uterine tissue section. Stained with Desmin (green) and Cytokeratin (red) using VectaFluor(TM) Duet Double Labeling Kit and vasculature usind DyLight 649 UEA I lectin (purple). Mounted in VECTASHIELD Vibrance with DAPI (blue).](http://images.novusbio.com/fullsize/VECTASHIELD-R-Vibrance-TM-Antifade-Mounting-Medium-with-DAPI-H-1800-10-NB-img0001.jpg "VECTASHIELD® Vibrance(TM) Antifade Mounting Medium with DAPI [H-1800-10-NB] - Human FFPE Uterine tissue section. Stained with Desmin (green) and Cytokeratin (red) using VectaFluor(TM) Duet Double Labeling Kit and vasculature usind DyLight 649 UEA I lectin (purple). Mounted in VECTASHIELD Vibrance with DAPI (blue).")
![VECTASHIELD® Vibrance(TM) Antifade Mounting Medium with DAPI [H-1800-10-NB] - Human FFPE colon tissue section stained for cytokeratin (green) using fluorescein anti-mouse IgG secondary Ab. Mounted in VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (blue).](http://images.novusbio.com/fullsize/VECTASHIELD-R-Vibrance-TM-Antifade-Mounting-Medium-with-DAPI-H-1800-10-NB-img0002.jpg)
![VECTASHIELD® Vibrance(TM) Antifade Mounting Medium with DAPI [H-1800-10-NB]](http://images.novusbio.com/fullsize/VECTASHIELD-R-Vibrance-TM-Antifade-Mounting-Medium-with-DAPI-H-1800-10-NB-img0004.jpg)