VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Summary
| Description |
VECTASHIELD Hardset Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. VECTASHIELD Hardset Mounting Medium with DAPI prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.
Features:
Inhibits photobleaching of fluorescent dyes and fluorescent proteins
Ideal refractive index (1.45)
Ready-to-use
No warming necessary
Can be stored without sealing for long term analysis
Hardening formulation
DAPI counterstain
VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.
VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.
|
Applications/Dilutions
| Dilutions |
- Cellular Imaging
- Immunocytochemistry/ Immunofluorescence
- In-situ Hybridization
|
| Application Notes |
Refractive index is 1.46.
NOTE: This product has a six month expiration date.. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
Notes
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0060.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Medium](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0067.jpg "Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Medium")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0041.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0042.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0043.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0044.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 594 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0045.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 594 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0046.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 594 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0047.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 594 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 549 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0048.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 549 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 488 labeled secondary antibody (green). S100, DyLight 594 labeled secondary antibody (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0049.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 488 labeled secondary antibody (green). S100, DyLight 594 labeled secondary antibody (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0051.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0052.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0053.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0054.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0055.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0056.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0057.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0058.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*")
![Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 549 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunocytochemistry-Immunofluorescence-H-1500-NB-img0059.jpg "Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 549 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*")
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Texas Red Avidin DCS (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0061.jpg "Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Texas Red Avidin DCS (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Medium](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0062.jpg "Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Medium")
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Mediu](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0063.jpg "Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Mediu")
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Medium](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0064.jpg "Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Medium")
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Retina from mice, perfused with DyLight 594-LEL. The retina were sectioned and mounted in Vectashield HardSet+DAPI. Image courtesy of George W. Smith, Florida Atlantic University](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0065.jpg "Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Retina from mice, perfused with DyLight 594-LEL. The retina were sectioned and mounted in Vectashield HardSet+DAPI. Image courtesy of George W. Smith, Florida Atlantic University")
![Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Texas Red Avidin DCS (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Immunohistochemistry-H-1500-NB-img0066.jpg "Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Texas Red Avidin DCS (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.")
![Cellular Imaging: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 488 labeled secondary antibody (green). S100, DyLight 594 labeled secondary antibody (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI](http://images.novusbio.com/fullsize/VECTASHIELD-R-Hard-Set-TM-Antifade-Mounting-Medium-with-DAPI-Cellular-Imaging-H-1500-NB-img0050.jpg "Cellular Imaging: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 488 labeled secondary antibody (green). S100, DyLight 594 labeled secondary antibody (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI")
