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![Western Blot: Seryl tRNA synthetase Antibody (1H4) [H00006301-M01] - SARS monoclonal antibody (M01), clone 1H4 Analysis of SARS expression in Jurkat.](http://images.novusbio.com/fullsize/Seryl-tRNA-synthetase-Antibody-1H4-Western-Blot-H00006301-M01-img0013.jpg "Western Blot: Seryl tRNA synthetase Antibody (1H4) [H00006301-M01] - SARS monoclonal antibody (M01), clone 1H4 Analysis of SARS expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: Seryl tRNA synthetase Antibody (1H4) [H00006301-M01] - Analysis of monoclonal antibody to SARS on HeLa cell. Antibody concentration 25 ug/ml.](http://images.novusbio.com/fullsize/Seryl-tRNA-synthetase-Antibody-1H4-Immunocytochemistry-Immunofluorescence-H00006301-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: Seryl tRNA synthetase Antibody (1H4) [H00006301-M01] - Analysis of monoclonal antibody to SARS on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Seryl-tRNA-synthetase-Antibody-1H4-Immunohistochemistry-Paraffin-H00006301-M01-img0010.jpg)
![Western Blot: Seryl tRNA synthetase Antibody (1H4) [H00006301-M01] - Analysis of SARS over-expressed 293 cell line, cotransfected with SARS Validated Chimera RNAi ( Cat # H00006301-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SARS monoclonal antibody (M01), clone 1H4 (Cat # H00006301-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Seryl-tRNA-synthetase-Antibody-1H4-Western-Blot-H00006301-M01-img0011.jpg)
![Western Blot: Seryl tRNA synthetase Antibody (1H4) [H00006301-M01] - Analysis of SARS expression in transfected 293T cell line by SARS monoclonal antibody (M01), clone 1H4.Lane 1: SARS transfected lysate(58.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Seryl-tRNA-synthetase-Antibody-1H4-Western-Blot-H00006301-M01-img0014.jpg)
![ELISA: Seryl tRNA synthetase Antibody (1H4) [H00006301-M01] - Detection limit for recombinant GST tagged SARS is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Seryl-tRNA-synthetase-Antibody-1H4-ELISA-H00006301-M01-img0015.jpg)
![Immunohistochemistry: Seryl tRNA synthetase Antibody [NBP3-35334] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Seryl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35334_rabbit-seryl-trna-synthetase-pab-101220241243923.jpg "Immunohistochemistry: Seryl tRNA synthetase Antibody [NBP3-35334] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Seryl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Seryl tRNA synthetase Antibody [NBP3-35334] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Seryl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35334_rabbit-seryl-trna-synthetase-pab-10122024122410.jpg)
![Western Blot: Seryl tRNA synthetase Antibody [NBP3-35334] - Western blot analysis of various lysates using Seryl tRNA synthetase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35334_rabbit-seryl-trna-synthetase-pab-101220241224113.jpg)
![Immunohistochemistry: Seryl tRNA synthetase Antibody [NBP3-35334] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using Seryl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35334_rabbit-seryl-trna-synthetase-pab-1012202411572615.jpg)
![Immunocytochemistry/ Immunofluorescence: Seryl tRNA synthetase Antibody [NBP3-38321] - Immunofluorescence analysis of MCF7 cells using Seryl tRNA synthetase Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38321_rabbit-seryl-trna-synthetase-pab-1012202411404038.jpg "Immunocytochemistry/ Immunofluorescence: Seryl tRNA synthetase Antibody [NBP3-38321] - Immunofluorescence analysis of MCF7 cells using Seryl tRNA synthetase Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: Seryl tRNA synthetase Antibody [NBP3-38321] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using Seryl tRNA synthetase Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38321_rabbit-seryl-trna-synthetase-pab-101220241136026.jpg)
![Western Blot: Seryl tRNA synthetase Antibody [NBP3-38321] - Western blot analysis of various lysates using Seryl tRNA synthetase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38321_rabbit-seryl-trna-synthetase-pab-1012202411404050.jpg)
![Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Simple-Western-NB100-56683-img0004.jpg "Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56683] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S [848561]); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Dual-RNAscope-ISH-IHC-NB100-56683-img0005.jpg)
![Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows lysates of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100). A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) only in the SARS-CoV-2 lysate using 25 ug/mL of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Note: some reactivity observed with FL Std 230. SARS-CoV-2 lysate courtesy of University of Maryland.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Simple-Western-NB100-56683-img0003.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56683] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody at a 1:2000 dilution.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56683-img0002.jpg)
![Western Blot: Sars Spike Protein Antibody [NB100-56047] - Analysis of SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody. 5 ug/ml.](http://images.novusbio.com/fullsize/Sars-Spike-Protein-Antibody-Western-Blot-NB100-56047-img0003.jpg "Western Blot: Sars Spike Protein Antibody [NB100-56047] - Analysis of SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody. 5 ug/ml.")
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunohistochemistry-NBP3-05695-img0003.jpg "Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.")
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody[NBP2-44205], showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP3-05695-img0001.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody [NB100-56683] at a 1:2000 dilution.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Western-Blot-NBP3-05695-img0004.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody[NBP2-44205] at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Western-Blot-NBP3-05695-img0006.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunohistochemistry-NBP3-05695-img0005.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628 SARS Nucleocapsid Protein Antibody [NB100-56576]](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunohistochemistry-NBP3-05695-img0002.jpg)
![Western Blot: Sars Spike Protein Antibody [NB100-56048] - Used at 1 ug/ml to analyze SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate.](http://images.novusbio.com/fullsize/Sars-Spike-Protein-Antibody-Western-Blot-NB100-56048-img0002.jpg "Western Blot: Sars Spike Protein Antibody [NB100-56048] - Used at 1 ug/ml to analyze SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate.")
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56049] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56049-img0002.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56049] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.")
![Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NBP2-44205-img0004.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44205-img0003.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NBP2-44205-img0005.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western Blot of Rabbit Anti-SARS CoV Nucleocapsid (N) Protein Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: SARS CoV Nucleocapsid (N) Protein [20 ng]. Primary Antibody: Anti-SARS CoV Nucleocapsid (N) Protein Antibody at 1.0 ug/mL overnight at 2-8 degrees C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40000 for 30 mins at RT. Predicted MW: ~46 kDa. Observed MW: ~48 kDa.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NBP2-44205-img0001.jpg)
![ELISA: SARS Nucleocapsid Protein Antibody [NBP2-44205] - ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein. Each well was coated in duplicate with 0.5ug/mL of SARS CoV-1 [Red Line], SARS CoV-2 Nc [Green Line], MERS CoV N [Blue Line]. The starting dilution of antibody was 2 ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV-1N IC50: 11ng/mL, SARS CoV-2N IC50: 15 ng/mL. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) and TMB substrate (p/n TMBE-1000).](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-ELISA-NBP2-44205-img0006.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56050] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56050-img0002.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56050] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.")