![Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - ZNF174 monoclonal antibody (M01), clone 2D7-E9 Analysis of ZNF174 expression in SW-13.](http://images.novusbio.com/fullsize/ZNF174-Antibody-3E4-Western-Blot-H00007727-M01-img0011.jpg "Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - ZNF174 monoclonal antibody (M01), clone 2D7-E9 Analysis of ZNF174 expression in SW-13.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, KD |
| Clone | 2D7-E9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | ZNF174 (AAH00876, 1 a.a. ~ 234 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAKMEITLSSNTEASSKQERHIIAKLEEKRGPPLQKNCPDPELCRQSFRRFCYQEVSGPQEALSQLRQLCRQWLQPELHTKEQILELLVMEQFLTILPPEIQARVRHRCPMSSKEIVTLVEDFHRASKKPKQWVAVCMQGQKVLLEKTGSQLGEQELPDFQPQTPRRDLRESSPAEPSQAGAYDRLSPHHWEKSPLLQEPTPKLAGTELLIEKTDPNMATDELPCKLWLSFIA |
| Localization | Nuclear |
| Specificity | ZNF174 - zinc finger protein 174 |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ZNF174 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of monoclonal antibody to ZNF174 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ZNF174-Antibody-3E4-Immunocytochemistry-Immunofluorescence-H00007727-M01-img0008.jpg "Immunocytochemistry/Immunofluorescence: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of monoclonal antibody to ZNF174 on HeLa cell. Antibody concentration 10 ug/ml.")
![Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of ZNF174 over-expressed 293 cell line, cotransfected with ZNF174 Validated Chimera RNAi ( Cat # H00007727-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF174 monoclonal antibody (M01), clone 2D7-E9 (Cat # H00007727-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/ZNF174-Antibody-3E4-Western-Blot-H00007727-M01-img0009.jpg "Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of ZNF174 over-expressed 293 cell line, cotransfected with ZNF174 Validated Chimera RNAi ( Cat # H00007727-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF174 monoclonal antibody (M01), clone 2D7-E9 (Cat # H00007727-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of ZNF174 expression in transfected 293T cell line by ZNF174 monoclonal antibody (M01), clone 2D7-E9.Lane 1: ZNF174 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZNF174-Antibody-3E4-Western-Blot-H00007727-M01-img0012.jpg "Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of ZNF174 expression in transfected 293T cell line by ZNF174 monoclonal antibody (M01), clone 2D7-E9.Lane 1: ZNF174 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: ZNF174 Antibody (3E4) [H00007727-M01] - Detection limit for recombinant GST tagged ZNF174 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ZNF174-Antibody-3E4-Sandwich-ELISA-H00007727-M01-img0010.jpg "Sandwich ELISA: ZNF174 Antibody (3E4) [H00007727-M01] - Detection limit for recombinant GST tagged ZNF174 is approximately 0.3ng/ml as a capture antibody.")
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