| Reactivity | HuSpecies Glossary |
| Applications | PAGE |
| Format | Carrier-Free |
| Description | A single non-glycosylated polypeptide chain containing 158 amino acids corresponding to UBE2I/Ubc9 and 8 aa vector sequence including 6x His tag at N-terminus Source: E. coli Amino Acid Sequence: MHHHHHHAMG TLNMSGIALS RLAQERKAWR KDHPFGFVAV PTKNPDGTMN LMNWECAIPG KKGTPWEGGL FKLRMLFKDD YPSSPPKCKF EPPLFHPNVY PSGTVCLSIL EEDKDWRPAI TIKQILLGIQ ELLNEPNIQD PAQAEAYTIY CQNRVEYEKR VRAQAKKFAP S |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | UBE2I |
| Purity | >95%, by SDS-PAGE and HPLC |
| Endotoxin Note | Less than 1 EU/ug of UBE2I/Ubc9 as determined by LAL method. |
| Dilutions |
|
| Theoretical MW | 19.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.6, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT. |
| Preservative | No Preservative |
| Purity | >95%, by SDS-PAGE and HPLC |
| Reconstitution Instructions | Please contact technical support for detailed reconstitution instructions. |
Research Areas for UBE2I/Ubc9 Recombinant Protein (NBP2-35094)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | UBE2I |
![SDS-Page: Human UBE2I/Ubc9 His (N-Term) Protein [NBP2-35094]](http://images.novusbio.com/fullsize/Human-UBE2I-Ubc9-His-N-Term-Protein-SDS-Page-NBP2-35094-img0001.jpg "SDS-Page: Human UBE2I/Ubc9 His (N-Term) Protein [NBP2-35094]")
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
![Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.](https://images.novusbio.com/images/UBE2I-Ubc9-Antibody-Western-Blot-NB300-812-img0007.jpg "Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.")