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Recombinant Human Troponin C (cardiac) His Protein

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SDS-Page: Recombinant Human Troponin C (cardiac) Protein [NBP1-72499] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human Troponin C (cardiac) His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-161 of Human Troponin C (cardiac)

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEFLEFMKGV E

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TNNC1
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human Troponin C (cardiac) His Protein

  • cardiac troponin C
  • CMD1Z
  • CMH13
  • slow twitch skeletal/cardiac muscle troponin C
  • TNC
  • TN-C
  • TNNC
  • troponin C type 1 (slow)
  • troponin C, slow skeletal and cardiac muscles
  • troponin C, slow
  • troponin C1, slow

Background

Troponin C type 1, also known as TNNC1, is a member of troponins family. Troponin has 3 subunits, Troponin I(Tn-I), Troponin T(Tn-T) and Troponin C(Tn-C). Tn-I subunit inhibits actomyosin ATPase and Tn-T subunit binds tropomyosin and Tn-C, while Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. TNNC1 is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Recombinant human TNNC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TNNC1