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Recombinant Human TRAILR2/TNFRSF10B Isoform 1 hIgG-His Protein

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SDS-Page: Recombinant Human TRAIL R2/TNFRSF10B Isoform 1 Protein [NBP2-61983] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
1 mg/ml

Order Details

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Recombinant Human TRAILR2/TNFRSF10B Isoform 1 hIgG-His Protein Summary

Description
A Biologically active recombinant protein with a C-Terminal hIgG-His-tag and corresponding to the amino acids 56-210 of Human TRAIL R2/TNFRSF10B

Source:Baculovirus

Amino Acid Sequence: ITQQDLAPQQ RAAPQQKRSS PSEGLCPPGH HISEDGRDCI SCKYGQDYST HWNDLLFCLR CTRCDSGEVE LSPCTTTRNT VCQCEEGTFR EEDSPEMCRK CRTGCPRGMV KVGDCTPWSD IECVHKESGT KHSGEVPAVE ETVTSSPGTP ASPCSLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH

Details of Functionality
Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells. The ED50 range is less than or equal to 5 ng/ml with Human TRAIL.
Source
Baculovirus
Protein/Peptide Type
Recombinant Protein
Gene
TNFRSF10B
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
43.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human TRAILR2/TNFRSF10B Isoform 1 hIgG-His Protein

  • CD262 antigen
  • CD262
  • death domain containing receptor for TRAIL/Apo-2L
  • Death receptor 5
  • DR5
  • DR5TRICK2B
  • Fas-like protein
  • KILLER/DR5
  • KILLERapoptosis inducing protein TRICK2A/2B
  • TNF-related apoptosis-inducing ligand receptor 2
  • TNFRSF10B
  • TRAIL R2
  • TRAIL receptor 2
  • TRAILR2
  • TRAIL-R2apoptosis inducing receptor TRAIL-R2
  • TRAILR2cytotoxic TRAIL receptor-2
  • TRICK2
  • TRICK2A
  • TRICKB
  • tumor necrosis factor receptor superfamily member 10B
  • tumor necrosis factor receptor superfamily, member 10b
  • tumor necrosis factor receptor-like protein ZTNFR9
  • ZTNFR9

Background

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-2). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TNFRSF10B