Recombinant Human TFF1/pS2 Protein Summary
Description |
A homodimer consisting of two 60 amino acid polypeptide chains corresponding to TFF1/pS2, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds Source: E. coli Uniprot ID: P04155 Amino Acid Sequence: EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles. |
Details of Functionality |
TFF1/pS2 protein is fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ug/ml, corresponding to a specific activity of >100 IU/mg |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
TFF1 |
Purity |
> 97 % pure by SDS-PAGE and HPLC |
Endotoxin Note |
Less than 1 EU/ug of TFF1/pS2 as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
13.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
> 97 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions |
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Alternate Names for Recombinant Human TFF1/pS2 Protein
Background
Estrogen Inducible Protein pS2 (Trefoil factor 1 or TFF1) belongs to the trefoil factor family that consists of three members named TFF1, TFF2 and TFF3. They are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. The TFFs are stable secretory proteins expressed highly in the gastrointestinal tract (gastric mucosa). Estrogen Inducible Protein pS2 (TFF1) is an essential protein for normal differentiation of the antral and pyloric gastric mucosa and functions as a stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. It is studied because of commonly expressed in tumors as well.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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