![Western Blot: SV40 T-antigen Antibody [NBP3-13382] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SV40 Large T antigen antibody (NBP3-13382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SV40-T-antigen-Antibody-Western-Blot-NBP3-13382-img0001.jpg "Western Blot: SV40 T-antigen Antibody [NBP3-13382] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SV40 Large T antigen antibody (NBP3-13382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of SV40 T-antigen (SV40 virus (strain 776)). The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 1% BSA, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: SV40 T-antigen Antibody [NBP3-13382] - SV40 T-antigen antibody detects SV40 T-antigen protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SV40 T-antigen stained by SV40 T-antigen antibody (NBP3-13382) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/SV40-T-antigen-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13382-img0002.jpg "Immunocytochemistry/Immunofluorescence: SV40 T-antigen Antibody [NBP3-13382] - SV40 T-antigen antibody detects SV40 T-antigen protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SV40 T-antigen stained by SV40 T-antigen antibody (NBP3-13382) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")