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Sulfatase Modifying Factor 1/SUMF1 Antibody

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Western Blot: Sulfatase Modifying Factor 1/SUMF1 Antibody [NBP1-45212] - Analysis of SUMF1 in Mouse Pancreas lysate (35ug protein in RIPA buffer) using NBP1-45212 at 1ug/ml. Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Sulfatase Modifying Factor 1/SUMF1 Antibody Summary

Immunogen
Peptide with sequence C-ETLNPKGPPSGKDR corresponding to internal region according to NP_877437.2.
Epitope
internal region
Predicted Species
Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SUMF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 2 ug/ml
Application Notes
WB: Approx. 37-40 kDa band observed in mouse pancreas, mouse eye and human kidney lysates (calculated MW of 40.6 kDa band according to human NP_877437.2 and 40.6 kDa band according to mouse NP_666049.2).
Publications
Read Publication using NBP1-45212.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Sulfatase Modifying Factor 1/SUMF1 Antibody

  • AAPA3037
  • EC 1.8.99.-
  • FGE1
  • FGEC-alpha-formylglycine-generating enzyme 1
  • FGly-generating enzyme
  • MGC131853
  • MGC150436
  • sulfatase modifying factor 1
  • sulfatase-modifying factor 1
  • SUMF1
  • UNQ3037

Background

Sulfatases catalyze the hydrolysis of sulfate esters such as glycosaminoglycans, sulfolipids, and steroid sulfates. C-alpha-formylglycine (FGly), the catalytic residue in the active site of eukaryotic sulfatases, is posttranslationally generated from a cysteine by SUMF1, the FGly-generating enzyme (FGE), in the endoplasmic reticulum (ER). The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in multiple sulfatase deficiency (MSD; MIM 272200), a lysosomal storage disorder (Roeser et al., 2006 [PubMed 16368756]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sulfatase Modifying Factor 1/SUMF1 Antibody (NBP1-45212)(1)

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Bioinformatics

Gene Symbol SUMF1
Entrez
Uniprot