Novus Biologicals products are now on bio-techne.com

SRD5A1 Antibody

Images

 
Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, GpSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

SRD5A1 Antibody Summary

Immunogen
Peptide with sequence ATATGVAEERLLC corresponding to N-Terminus according to NP_001038.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SRD5A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4-6 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1:100 - 1:2000
Application Notes
IHC: In paraffin embedded Human Prostate shows reticulate staining in the cytoplasm of secretory cells in the gland. Paraffin embedded Human Liver.
WB usage is reported in scientific literature (PMID: 21747118).
Control Peptide
SRD5A1 Peptide (NB100-1491PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB100-1491 in the following applications:

Publications
Read Publications using
NB100-1491 in the following applications:

  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in (PMID: 25797030). Guinea Pig reactivity reported in (PMID: 23585339).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SRD5A1 Antibody

  • 3-oxo-5-alpha-steroid 4-dehydrogenase 1
  • EC 1.3.1.22
  • EC 1.3.99.5,3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1
  • S5AR 1
  • SR type 1
  • SRD5A1
  • Steroid 5-alpha-reductase 1,5-alpha reductase
  • steroid 5-alpha-reductase type I
  • steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta4-dehydrogenase alpha 1)

Background

Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). There are 2 isoforms of the enzyme: SRD5A1 and SRD5A2 (MIM 607306).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

DKK300
Species: Hu
Applications: ELISA
NB100-56603
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
NBP1-46510
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-38530
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89146
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF262
Species: Hu
Applications: ICC, IHC, Neut, WB
AF4036
Species: Hu
Applications: Simple Western, WB
NBP1-32920
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-13669
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00005324-M02
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, WB
NB100-1596
Species: Hu, Mu, Pm, Rb, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
7678-DH
Species: Hu
Applications: EnzAct
AF5415
Species: Hu
Applications: ICC, IHC, Simple Western, WB
NBP1-32353
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-01151
Species: Hu
Applications: Flow, IHC, IHC-P, WB
291-G1
Species: Hu
Applications: BA
NBP3-35145
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
MAB6529
Species: Hu
Applications: Simple Western, WB
NB110-78644
Species: Pm, Bv, Gt, Hu, Mu, Po, Pm, Rt, Sh
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-85368
Species: Hu, RM
Applications: ICC/IF, IHC, IHC-P, WB

Publications for SRD5A1 Antibody (NB100-1491)(8)

We have publications tested in 2 confirmed species: Mouse, Guinea Pig.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
All Applications
Filter By Species
Mouse
(1)
Guinea Pig
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-1491 Applications Species
Cumberland AL, Palliser HK, Walker DW, Hirst JJ. Cerebellar Changes in Guinea Pig Offspring Following Suppression of Neurosteroid Synthesis During Late Gestation. Cerebellum 2016-06-03 [PMID: 27255705] (WB, Guinea Pig) WB Guinea Pig
Fokidis HB, Yieng Chin M, Ho VW et al. A low carbohydrate, high protein diet suppresses intratumoral androgen synthesis and slows castration-resistant prostate tumor growth in mice. J Steroid Biochem Mol Biol 2015-03-19 [PMID: 25797030] (WB, Mouse) WB Mouse
Linard C, Busson E, Holler V et al. Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs. Stem Cells Transl Med. 2013-11-01 [PMID: 24068742]
Kelleher MA, Hirst JJ, Palliser HK. Changes in Neuroactive Steroid Concentrations After Preterm Delivery in the Guinea Pig. Reprod Sci 2013-04-12 [PMID: 23585339] (WB, Guinea Pig) WB Guinea Pig
Lubik AA, Gunter JH, Hendy SC et al. Insulin increases de novo steroidogenesis in prostate cancer cells. Cancer Res 2011-07-14 [PMID: 21747118]
Kelleher MA, Palliser HK, Walker DW, Hirst JJ. Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on fetal guinea pig brain development. J Endocrinol. 2010-12-13 [PMID: 21149437]
Locke JA, Guns ES, Lubik AA et al. Androgen Levels Increase by Intratumoral De novo Steroidogenesis during Progression of Castration-Resistant Prostate Cancer. Cancer Res 2008-08-01 [PMID: 18676866]
Iczkowski KA, Qiu J, Qian J et al. The dual 5-alpha-reductase inhibitor dutasteride induces atrophic changes and decreases relative cancer volume in human prostate. Urology 2005-01-01 [PMID: 15667867]

Review for SRD5A1 Antibody (NB100-1491) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1491:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin SRD5A1 NB100-1491
Enlarge
4
reviewed by:
Paula Aliberti
IHC-P Human 10/09/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedAdrenal gland tissue
SpeciesHuman
LotG2E300816

Comments

Commentsprimary Ab diluted 1/50

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SRD5A1 Antibody (NB100-1491) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SRD5A1 Products

Research Areas for SRD5A1 Antibody (NB100-1491)

Find related products by research area.

Blogs on SRD5A1

There are no specific blogs for SRD5A1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Paula Aliberti
10/09/2017
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SRD5A1
Entrez
Uniprot