SRD5A1 Antibody Summary
Immunogen
Peptide with sequence ATATGVAEERLLC corresponding to N-Terminus according to NP_001038.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SRD5A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Applications/Dilutions
Dilutions
Immunohistochemistry 4 - 6 ug/ml Immunohistochemistry-Paraffin 4-6 ug/ml Peptide ELISA Detection limit 1:128000 Western Blot 1:100 - 1:2000
Application Notes
IHC: In paraffin embedded Human Prostate shows reticulate staining in the cytoplasm of secretory cells in the gland. Paraffin embedded Human Liver. WB usage is reported in scientific literature (PMID: 21747118).
Control Peptide
Reviewed Applications
Read 1 Review rated 4
using NB100-1491 in the following applications:
Publications
Read Publications using NB100-1491 in the following applications:
Reactivity Notes
Mouse reactivity reported in (PMID: 25797030). Guinea Pig reactivity reported in (PMID: 23585339).
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified
Alternate Names for SRD5A1 Antibody
Background
Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). There are 2 isoforms of the enzyme: SRD5A1 and SRD5A2 (MIM 607306).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for SRD5A1 Antibody (NB100-1491)(8)
We have publications tested in 2 confirmed species: Mouse, Guinea Pig.
We have publications tested in 1 application: WB.
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Showing Publications 1 -
8 of 8.
Publications using NB100-1491
Applications
Species
Cumberland AL, Palliser HK, Walker DW, Hirst JJ. Cerebellar Changes in Guinea Pig Offspring Following Suppression of Neurosteroid Synthesis During Late Gestation. Cerebellum 2016-06-03 [PMID: 27255705] (WB, Guinea Pig)
WB
Guinea Pig
Fokidis HB, Yieng Chin M, Ho VW et al. A low carbohydrate, high protein diet suppresses intratumoral androgen synthesis and slows castration-resistant prostate tumor growth in mice. J Steroid Biochem Mol Biol 2015-03-19 [PMID: 25797030] (WB, Mouse)
WB
Mouse
Linard C, Busson E, Holler V et al. Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs. Stem Cells Transl Med. 2013-11-01 [PMID: 24068742]
Kelleher MA, Hirst JJ, Palliser HK. Changes in Neuroactive Steroid Concentrations After Preterm Delivery in the Guinea Pig. Reprod Sci 2013-04-12 [PMID: 23585339] (WB, Guinea Pig)
WB
Guinea Pig
Lubik AA, Gunter JH, Hendy SC et al. Insulin increases de novo steroidogenesis in prostate cancer cells. Cancer Res 2011-07-14 [PMID: 21747118]
Kelleher MA, Palliser HK, Walker DW, Hirst JJ. Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on fetal guinea pig brain development. J Endocrinol. 2010-12-13 [PMID: 21149437]
Locke JA, Guns ES, Lubik AA et al. Androgen Levels Increase by Intratumoral De novo Steroidogenesis during Progression of Castration-Resistant Prostate Cancer. Cancer Res 2008-08-01 [PMID: 18676866]
Iczkowski KA, Qiu J, Qian J et al. The dual 5-alpha-reductase inhibitor dutasteride induces atrophic changes and decreases relative cancer volume in human prostate. Urology 2005-01-01 [PMID: 15667867]
Review for SRD5A1 Antibody (NB100-1491) (1)
4 1
Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
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Reviews using NB100-1491:
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Images
Ratings
Applications
Species
Date
Details
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4
reviewed by: Paula Aliberti
IHC-P
Human
10/09/2017
Summary
Application Immunohistochemistry-Paraffin Sample Tested Adrenal gland tissue Species Human Lot G2E300816
Comments
Comments primary Ab diluted 1/50
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