![Western Blot: SRD5A2 Antibody [NBP1-46510] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:4000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/SRD5A2-Antibody-Western-Blot-NBP1-46510-img0010.jpg "Western Blot: SRD5A2 Antibody [NBP1-46510] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:4000 incubated at 4C overnight.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | A synthetic peptide from amino acid region 190-250 of human SRD5A2 conjugated to blue carrier protein was used as the antigen. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SRD5A2 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The optimal dilution should be determined by the end user. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin/Frozen. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from whole antisera |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Unpurified |
| Reconstitution Instructions | Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format. |
Secondary Antibodies |
Isotype Controls |
Research Areas for SRD5A2 Antibody (NBP1-46510)Find related products by research area.
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![Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/SRD5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-46510-img0009.jpg "Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human pancreas. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using using HRP polymer following manufacturers inctructions. DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/SRD5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-46510-img0001.jpg "Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human pancreas. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using using HRP polymer following manufacturers inctructions. DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human pancreas. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/SRD5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-46510-img0002.jpg "Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human pancreas. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/SRD5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-46510-img0003.jpg "Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/SRD5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-46510-img0004.jpg "Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/SRD5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-46510-img0005.jpg "Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/SRD5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-46510-img0006.jpg "Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/SRD5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-46510-img0007.jpg "Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/SRD5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-46510-img0008.jpg "Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")