![Western Blot: SPG20 Antibody [NBP1-47203] - (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SPG20-Antibody-Western-Blot-NBP1-47203-img0005.jpg "Western Blot: SPG20 Antibody [NBP1-47203] - (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, KD |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-EASGTDVKQLDQGNK corresponding to internal region according to NP_055902.1. |
| Epitope | C-EASGTDVKQLDQGNK |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SPART |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IF: HeLa show dimished signals after knock down of expresssion by introduction of siRNA. WB: Approx. 70 kDa band observed in HeLa lysates (calculated MW of 72.8 kDa band according to NP_055902.1). |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-47203 | Applications | Species |
|---|---|---|
| Lu J, Rashid F, Byrne PC. The hereditary spastic paraplegia protein spartin localises to mitochondria J Neurochem 2006-09-01 [PMID: 16945107] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SPART |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: SPG20 Antibody [NBP1-47203] - (2ug/ml) staining of methanol-fixed HeLa before (left) and after (right) introduction of siRNA.](http://images.novusbio.com/fullsize/SPG20-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47203-img0004.jpg "Immunocytochemistry/Immunofluorescence: SPG20 Antibody [NBP1-47203] - (2ug/ml) staining of methanol-fixed HeLa before (left) and after (right) introduction of siRNA.")
