Home » Semaphorin 3A » Semaphorin 3A Lysates » Semaphorin 3A Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

Semaphorin 3A Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: SEMA3A Overexpression Lysate (Adult Normal) [NBP2-07669] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEMA3A.
Western Blot: SEMA3A Overexpression Lysate (Adult Normal) [NBP2-07669] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEMA3A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

100% Guarantee on every product

Semaphorin 3A Overexpression Lysate Summary

Description

Semaphorin 3A Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC213681

Accession#: NM_006080

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
SEMA3A

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
86.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Semaphorin 3A Overexpression Lysate

  • (semaphorin) 3A
  • coll-1
  • collapsin 1
  • Hsema-I
  • Hsema-III
  • MGC133243
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted
  • Sema III
  • SEMA1
  • Sema3A
  • SEMAD
  • SEMAIII
  • SEMAL
  • Semaphorin 3A
  • semaphorin D
  • Semaphorin III
  • semaphorin L
  • semaphorin-3A
  • SemD

Background

SEMA3A - sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Neuropilin-1
Antibody [Unconjugate
AF566
Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]
Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]
Western Blot Neuropilin-1 Antibody [Unconjugated]
(10 Reviews)
(103 Publications)
Species: Mu, Rt
Applications: Block, CyTOF-ready, Flow, IHC, WB
Semaphorin 3A
[Unconjugated]
1250-S3
Bioactivity Semaphorin 3A [Unconjugated]
(33 Publications)
Species: Hu
Applications: BA, BA
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(672 Publications)
Species: Hu
Applications: ELISA
Neuropilin-2
Antibody [Unconjugate
AF567
Immunohistochemistry Neuropilin-2 Antibody [Unconjugated]
Western Blot Neuropilin-2 Antibody [Unconjugated]
Simple Western Neuropilin-2 Antibody [Unconjugated]
(4 Reviews)
(39 Publications)
Species: Mu, Rt
Applications: Block, IHC, Simple Western, WB
Plexin A1 Antibody
[Unconjugated]
AF4309
Immunohistochemistry Plexin A1 Antibody [Unconjugated]
Immunocytochemistry Plexin A1 Antibody [Unconjugated]
Western Blot Plexin A1 Antibody [Unconjugated]
(11 Publications)
Species: Hu, Mu
Applications: ICC, IHC, WB
NELL1 Antibody
AF7109
Western Blot NELL1 Antibody
Immunohistochemistry NELL1 Antibody
Species: Mu
Applications: IHC, WB
Semaphorin 3F
[Unconjugated]
3237-S3
Semaphorin 3F [Unconjugated]
(8 Publications)
Species: Mu
Applications: BA
Semaphorin 3C
Antibody (238835) [Un ...
MAB1728
Immunocytochemistry Semaphorin 3C Antibody (238835) [Unconjugated]
Immunohistochemistry Semaphorin 3C Antibody (238835) [Unconjugated]
(6 Publications)
Species: Hu, Mu
Applications: ICC, IHC, WB
Semaphorin 3B
Antibody
NB100-2218
Simple Western Semaphorin 3B Antibody
Western Blot Semaphorin 3B Antibody
Immunocytochemistry/ Immunofluorescence Semaphorin 3B Antibody
(2 Publications)
Species: Bv, Ca, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
beta-NGF
[Unconjugated]
256-GF
SDS-PAGE beta-NGF [Unconjugated]
Bioactivity beta-NGF [Unconjugated]
(57 Publications)
Species: Hu
Applications: BA
CRMP2 Antibody
NBP1-85448
Western Blot CRMP2 Antibody
Western Blot CRMP2 Antibody
Immunohistochemistry-Paraffin CRMP2 Antibody
(2 Reviews)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Netrin-1
[Unconjugated]
1109-N1
SDS-PAGE Netrin-1 [Unconjugated]
(40 Publications)
Species: Mu
Applications: Bind
Semaphorin 6A
Antibody [Unconjugate
AF1615
Immunohistochemistry Semaphorin 6A Antibody [Unconjugated]
(7 Publications)
Species: Mu
Applications: IHC, WB
BDNF [HRP]
DBD00
N/A BDNF [HRP]
N/A BDNF [HRP]
N/A BDNF [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
Plexin A4 Antibody
(707201) [Unconjugate ...
MAB5856
Western Blot Plexin A4 Antibody (707201) [Unconjugated]
Simple Western Plexin A4 Antibody (707201) [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: Simple Western, WB
Plexin A3 Antibody
[Unconjugated]
AF4075
Immunohistochemistry Plexin A3 Antibody [Unconjugated]
Immunohistochemistry Plexin A3 Antibody [Unconjugated]
(4 Publications)
Species: Mu, Rt
Applications: IHC, WB

Publications for Semaphorin 3A Lysate (NBP2-07669) (0)

There are no publications for Semaphorin 3A Lysate (NBP2-07669).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Semaphorin 3A Lysate (NBP2-07669) (0)

There are no reviews for Semaphorin 3A Lysate (NBP2-07669). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Semaphorin 3A Lysate (NBP2-07669) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Semaphorin 3A Products

Research Areas for Semaphorin 3A Lysate (NBP2-07669)

Find related products by research area.

Blogs on Semaphorin 3A

There are no specific blogs for Semaphorin 3A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Semaphorin 3A Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol SEMA3A