Sars Envelope Protein Antibody Summary
| Immunogen |
The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 59-70 YSRVKNLNSSEG of the putative SARS envelope protein. |
| Specificity |
The antibody was tested on a recombinant fusion protein containing amino acids 59-70 of SARS-E. |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Purity |
Protein G purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- ELISA 1-3 ug/ml
- Western Blot 1-3 ug/ml
|
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (83% (10/12))(YP_009724392.1)
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
PBS |
| Preservative |
0.05% Sodium Azide |
| Concentration |
0.5 mg/ml |
| Purity |
Protein G purified |
Alternate Names for Sars Envelope Protein Antibody
Background
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Publications for Sars Envelope Protein Antibody (NB100-56562) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Sars Envelope Protein Antibody (NB100-56562). (Showing 1 - 1 of 1 FAQ).
-
Hi! does NB100-56047 cross-react with Sars-2?
- The immunogen for NB100-56047 has no similarity to SARS-CoV-2. The immunogen for NB100-56562 one is 100% homologous.
Secondary Antibodies
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Isotype Controls
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Additional Sars Envelope Protein Products
Research Areas for Sars Envelope Protein Antibody (NB100-56562)
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Blogs on Sars Envelope Protein
![Western Blot: Sars Envelope Protein Antibody [NB100-56562] - analysis of SARS-E in (A) recombinant fusion protein containing amino acids 59-70 and (B) fusion partner without these amino acids, using this antibody.](http://images.novusbio.com/fullsize/Sars-Envelope-Protein-Antibody-Western-Blot-NB100-56562-img0002.jpg "Western Blot: Sars Envelope Protein Antibody [NB100-56562] - analysis of SARS-E in (A) recombinant fusion protein containing amino acids 59-70 and (B) fusion partner without these amino acids, using this antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")