![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (001) [NBP2-90988] - 1:2000 dilution. Lane A: SARS-CoV-2 NP Protein (30ng). Lane B: SARS-CoV NP Protein (5ng). Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng). Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng). Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-001-Western-Blot-NBP2-90988-img0001.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody (001) [NBP2-90988] - 1:2000 dilution. Lane A: SARS-CoV-2 NP Protein (30ng). Lane B: SARS-CoV NP Protein (5ng). Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng). Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng). Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.")
| Reactivity | V, VSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 001 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant SARS-CoV Nucleoprotein/NP Protein |
| Specificity | Has cross-reactivity in ELISA and WB with: SARS-CoV-2 Nucleocapsid Protein Has cross-reactivity in ELISA with: SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein SARS-CoV-2 Nucleocapsid Protein SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) Protein SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) Protein SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) Protein SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) Protein SARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) Protein SARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) Protein SARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) Protein SARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) Protein SARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) Protein SARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) Protein SARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) Protein SARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) Protein SARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) Protein No cross-reactivity in ELISA with: MERS-CoV Nucleocapsid protein HCoV-229E Nucleocapsid protein HCoV-NL63 Nucleocapsid protein HCoV-HKU1(isolate N5) Nucleocapsid protein HCoV-OC43 Nucleocapsid protein |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | N |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | N |
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARSNucleocapsid Rabbit Mab,then a HRPconjugated second step antibody](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-001-Immunocytochemistry-Immunofluorescence-NBP2-90988-img0003.jpg "Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARSNucleocapsid Rabbit Mab,then a HRPconjugated second step antibody")
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Rabbit Mab,then a Alexa Fluor®488-conjugated second step antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-001-Immunocytochemistry-Immunofluorescence-NBP2-90988-img0002.jpg "Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Rabbit Mab,then a Alexa Fluor®488-conjugated second step antibody.")
![ELISA: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Nucleocapsid-His recombinant Protein,Biotinylated on SA Biosensor, can bind SARSCoV/ SARS-CoV-2 Nucleocapsid Antibody, Rabbit MAb with an affinity constant of 0.02 nM as determined by Octet RED System.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-001-ELISA-NBP2-90988-img0004.jpg "ELISA: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Nucleocapsid-His recombinant Protein,Biotinylated on SA Biosensor, can bind SARSCoV/ SARS-CoV-2 Nucleocapsid Antibody, Rabbit MAb with an affinity constant of 0.02 nM as determined by Octet RED System.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")