![Immunohistochemistry-Paraffin: SARS-CoV-2 nsp1 Antibody (HL1435) - Azide and BSA Free [NBP3-25649] - SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] detects SARS-CoV-2 (COVID-19) nsp1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) nsp1 transfected 293T. Green: SARS-CoV-2 (COVID-19) nsp1 stained by SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] (NBP3-25649) diluted at 1:1000. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25649_rabbit-sars-cov-2-nsp1-mab-hl1435-azide-and-bsa-free-2120249415981.jpg "Immunohistochemistry-Paraffin: SARS-CoV-2 nsp1 Antibody (HL1435) - Azide and BSA Free [NBP3-25649] - SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] detects SARS-CoV-2 (COVID-19) nsp1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) nsp1 transfected 293T. Green: SARS-CoV-2 (COVID-19) nsp1 stained by SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] (NBP3-25649) diluted at 1:1000. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
| Reactivity | VSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1435 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of SARS-CoV-2 nsp1. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ORF1ab |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ORF1ab |
![Western Blot: SARS-CoV-2 nsp1 Antibody (HL1435) - Azide and BSA Free [NBP3-25649] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] (NBP3-25649) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25649_rabbit-sars-cov-2-nsp1-mab-hl1435-azide-and-bsa-free-2120249432873.jpg "Western Blot: SARS-CoV-2 nsp1 Antibody (HL1435) - Azide and BSA Free [NBP3-25649] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] (NBP3-25649) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp1 Antibody (HL1435) - Azide and BSA Free [NBP3-25649] - SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] detects SARS-CoV-2 (COVID-19) nsp1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp1 stained by SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] (NBP3-25649) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25649_rabbit-sars-cov-2-nsp1-mab-hl1435-azide-and-bsa-free-212024940842.jpg "Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp1 Antibody (HL1435) - Azide and BSA Free [NBP3-25649] - SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] detects SARS-CoV-2 (COVID-19) nsp1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp1 stained by SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] (NBP3-25649) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")