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Recombinant Human RNase H1 GST (N-Term) Protein

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Recombinant Human RNase H1 Protein [H00246243-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, MA, AP

Order Details

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Recombinant Human RNase H1 GST (N-Term) Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-286 of Human RNASEH1

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
RNASEH1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
Theoretical MW
58.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
H00246243-P01 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human RNase H1 GST (N-Term) Protein

  • EC 3.1.26.4
  • H1RNA
  • Ribonuclease H type II
  • ribonuclease H1
  • RNase H1
  • RNH1

Background

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for RNase H1 Recombinant Protein (H00246243-P01) (0)

There are no publications for RNase H1 Recombinant Protein (H00246243-P01).
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Review for RNase H1 Recombinant Protein (H00246243-P01) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using H00246243-P01:
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(1)
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  5
reviewed by:
Verified Customer
WB 08/07/2014
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Summary

ApplicationWestern Blot

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Verified Customer
08/07/2014
Application: WB
Species:

Bioinformatics

Gene Symbol RNASEH1