![Western Blot: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Analysis of RNH1 expression in transfected 293T cell line by RNH1 monoclonal antibody (M07), clone 3F5. Lane 1: RNH1 transfected lysatE (50 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-3F5-Western-Blot-H00006050-M07-img0007.jpg "Western Blot: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Analysis of RNH1 expression in transfected 293T cell line by RNH1 monoclonal antibody (M07), clone 3F5. Lane 1: RNH1 transfected lysatE (50 KDa). Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 3F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | RNH1 (AAH11500, 1 a.a. ~ 461 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGDVGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDPQCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCGVTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVLRAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISNNRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVESVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS |
| Specificity | RNH1 - ribonuclease/angiogenin inhibitor 1 (3F5) |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RNH1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00006050-M07 | Applications | Species |
|---|---|---|
| Furia A, Moscato M, Cali G et al. The ribonuclease/angiogenin inhibitor is also present in mitochondria and nuclei. FEBS Lett. 2011-01-26 [PMID: 21276451] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Analysis of RNH1 expression in K-562.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-3F5-Western-Blot-H00006050-M07-img0005.jpg "Western Blot: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Analysis of RNH1 expression in K-562.")
![Western Blot: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Analysis of RNH1 expression in human colon.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-3F5-Western-Blot-H00006050-M07-img0006.jpg "Western Blot: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Analysis of RNH1 expression in human colon.")
![Sandwich ELISA: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Detection limit for recombinant GST tagged RNH1 is 10 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-3F5-Sandwich-ELISA-H00006050-M07-img0004.jpg "Sandwich ELISA: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Detection limit for recombinant GST tagged RNH1 is 10 ng/ml as a capture antibody.")
![N/A Angiogenin [HRP]](https://images.novusbio.com/images2/Angiogenin_DAN00_ELISA_28.jpg)
![N/A Angiogenin [HRP]](https://images.novusbio.com/images2/DATA_Angiogenin_DAN00_ELISA_571.jpg)
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
