Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free Summary
| Additional Information |
Recombinant Monoclonal Antibody |
| Immunogen |
Recombinant protein encompassing a sequence within the center region of Respiratory Syncytial Virus Nucleoprotein. The exact sequence is proprietary. |
| Isotype |
IgG |
| Clonality |
Monoclonal |
| Host |
Rabbit |
| Purity |
Protein A purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- ELISA
- Immunocytochemistry/ Immunofluorescence
- Sandwich ELISA
- Western Blot 1:500-1:10000
|
Reactivity Notes
Respiratory Syncytial Virus
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
PBS |
| Preservative |
No Preservative |
| Purity |
Protein A purified |
Background
Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Publications for Respiratory Syncytial Virus Nucleoprotein Antibody (NBP3-25479) (0)
There are no publications for Respiratory Syncytial Virus Nucleoprotein Antibody (NBP3-25479).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Respiratory Syncytial Virus Nucleoprotein Antibody (NBP3-25479) (0)
There are no reviews for Respiratory Syncytial Virus Nucleoprotein Antibody (NBP3-25479).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Respiratory Syncytial Virus Nucleoprotein Antibody (NBP3-25479) (0)
Secondary Antibodies
| |
Isotype Controls
|
Additional Respiratory Syncytial Virus Nucleoprotein Products
Blogs on Respiratory Syncytial Virus Nucleoprotein
![Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25479_rabbit-respiratory-syncytial-virus-nucleoprotein-mab-hl1296-azide-bsa-free-212024940878.jpg "Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Indirect ELISA analysis performed by coating plate with recombinant HEK293 expressed, N-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10^-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect bound primary antibody. Antibodies: 1. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) 2. Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) 3. Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630)](http://images.novusbio.com/fullsize/nbp3-25479_rabbit-respiratory-syncytial-virus-nucleoprotein-mab-hl1296-azide-bsa-free-2120249374926.jpg "ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Indirect ELISA analysis performed by coating plate with recombinant HEK293 expressed, N-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10^-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect bound primary antibody. Antibodies: 1. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) 2. Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) 3. Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630)")
![ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.d with capture one. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25479_rabbit-respiratory-syncytial-virus-nucleoprotein-mab-hl1296-azide-bsa-free-212024940810.jpg "ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.d with capture one. Please contact us for custom HRP-conjugated antibody.")
![Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25479_rabbit-respiratory-syncytial-virus-nucleoprotein-mab-hl1296-azide-bsa-free-2120249403820.jpg "Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (NBP3-25626) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25479_rabbit-respiratory-syncytial-virus-nucleoprotein-mab-hl1296-azide-bsa-free-212024940881.jpg "ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (NBP3-25626) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.")
![Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Respiratory syncytial virus lysate (1 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25479_rabbit-respiratory-syncytial-virus-nucleoprotein-mab-hl1296-azide-bsa-free-2120249425428.jpg "Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Respiratory syncytial virus lysate (1 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP (1 ug/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP (1 ug/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25479_rabbit-respiratory-syncytial-virus-nucleoprotein-mab-hl1296-azide-bsa-free-2120249393519.jpg "ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP (1 ug/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP (1 ug/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.")
![ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25479_rabbit-respiratory-syncytial-virus-nucleoprotein-mab-hl1296-azide-bsa-free-2120249351361.jpg "ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.")
![ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (NBP3-25626) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (NBP3-25626) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (NBP3-25626) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25479_rabbit-respiratory-syncytial-virus-nucleoprotein-mab-hl1296-azide-bsa-free-2120249371585.jpg "ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1296) - Azide and BSA Free [NBP3-25479] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (NBP3-25626) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (NBP3-25626) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (NBP3-25626) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")