Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Mouse Shh encodes a 437 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Avian - Quail, Chicken, Primate - Macaca mulatta (Rhesus Macaque), Xenopus.

We have publications tested in 5 applications: Bioassay, Cell Culture, In Vivo, MALDI-TOF, Western Blot.

Showing Publications 1 - 10 of 36. Show All 36 Publications. Collapse Publications.

Publications using 461-SH	Applications	Species
K Takaya, N Aramaki-Ha, S Sakai, K Okabe, K Kishi Effect of Sonic Hedgehog on the Regeneration of Epidermal Texture Patterns Biomedicines, 2022-12-01;10(12):. 2022-12-01 [PMID: 36551853] (In Vivo, Mouse)	In Vivo	Mouse
FJ Gao, D Klinedinst, FX Fernandez, B Cheng, A Savonenko, B Devenney, Y Li, D Wu, MG Pomper, RH Reeves Forebrain Shh overexpression improves cognitive function and locomotor hyperactivity in an aneuploid mouse model of Down syndrome and its euploid littermates Acta neuropathologica communications, 2021-08-16;9(1):137. 2021-08-16 [PMID: 34399854] (MALDI-TOF, Western Blot)	MALDI-TOF, Western Blot
D Zhang, J Huang, F Wang, H Ding, Y Cui, Y Yang, J Xu, H Luo, Y Gao, L Pan, Y Wu, Y Gong, L Xie, Z Liu, Y Qu, L Zhang, W Liu, W Zhang, S Zhao, Q Yi, T Niu, Y Zheng BMI1 regulates multiple myeloma-associated macrophage's pro-myeloma functions Cell Death & Disease, 2021-05-15;12(5):495. 2021-05-15 [PMID: 33993198] (Bioassay, Mouse)	Bioassay	Mouse
K Song, Y Qing, Q Guo, EK Peden, C Chen, WE Mitch, L Truong, J Cheng PDGFRA in vascular adventitial MSCs promotes neointima formation in arteriovenous fistula in chronic kidney disease JCI Insight, 2020-11-05;0(0):. 2020-11-05 [PMID: 33001865] (Bioassay, Mouse)	Bioassay	Mouse
M Petroni, M Sahùn Ronc, V Ramponi, F Fabretti, V Nicolis Di, M Moretti, V Alfano, A Corsi, S De Panfili, M Giubettini, S Di Giulio, C Capalbo, F Belardinil, A Coppa, F Sardina, V Colicchia, F Pedretti, P Infante, B Cardinali, A Tessitore, G Canettieri, E De Smaele, G Giannini SMO-M2 mutation does not support cell-autonomous Hedgehog activity in cerebellar granule cell precursors Sci Rep, 2019-12-23;9(1):19623. 2019-12-23 [PMID: 31873117] (Bioassay, Mouse)	Bioassay	Mouse
M Matjusaiti, LJ Wagstaff, A Martella, B Baranowski, C Blin, S Gogolok, A Williams, SM Pollard Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors Stem Cell Reports, 2019-11-07;13(6):1053-1067. 2019-11-07 [PMID: 31708478] (Cell Culture, Mouse)	Cell Culture	Mouse
AJ Petty, A Li, X Wang, R Dai, B Heyman, D Hsu, X Huang, Y Yang Hedgehog signaling promotes tumor-associated macrophage polarization to suppress intratumoral CD8+ T cell recruitment J. Clin. Invest., 2019-12-02;0(0):. 2019-12-02 [PMID: 31638600] (Cell Culture, Mouse)	Cell Culture	Mouse
X Yuan, M Liu, X Cao, S Yang Ciliary IFT80 regulates dental pulp stem cells differentiation by FGF/FGFR1 and Hh/BMP2 signaling Int. J. Biol. Sci., 2019-08-06;15(10):2087-2099. 2019-08-06 [PMID: 31592124] (Bioassay, Mouse)	Bioassay	Mouse
C Xu, C Zou, M Hussain, W Shi, Y Shao, Z Jiang, X Wu, M Lu, J Wu, Q Xie, Y Ke, F Long, L Tang, X Wu High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia Mucosal Immunol, 2018-06-04;0(0):. 2018-06-04 [PMID: 29867080] (In Vivo, Mouse)	In Vivo	Mouse
D Gu, S Wang, S Zhang, P Zhang, G Zhou Directed transdifferentiation of M�ller glial cells to photoreceptors using the sonic hedgehog signaling pathway agonist purmorphamine Mol Med Rep, 2017-09-28;0(0):. 2017-09-28 [PMID: 28983586] (Bioassay, Rat)	Bioassay	Rat
A Solanki, CI Lau, JI Saldaña, S Ross, T Crompton The transcription factor Gli3 promotes B cell development in fetal liver through repression of Shh J. Exp. Med., 2017-05-22;0(0):. 2017-05-22 [PMID: 28533268] (Bioassay, Mouse)	Bioassay	Mouse
Lan T, Kisseleva T, Brenner D Deficiency of NOX1 or NOX4 Prevents Liver Inflammation and Fibrosis in Mice through Inhibition of Hepatic Stellate Cell Activation. PLoS ONE, 2015-07-29;10(7):e0129743. 2015-07-29 [PMID: 26222337] (Bioassay, Mouse)	Bioassay	Mouse
Chavez M, Ena S, Van Sande J, de Kerchove d'Exaerde A, Schurmans S, Schiffmann S Modulation of Ciliary Phosphoinositide Content Regulates Trafficking and Sonic Hedgehog Signaling Output. Dev Cell, 2015-07-16;34(3):338-50. 2015-07-16 [PMID: 26190144] (Bioassay, Mouse)	Bioassay	Mouse
Yuan X, Yang S Deletion of IFT80 Impairs Epiphyseal and Articular Cartilage Formation Due to Disruption of Chondrocyte Differentiation. PLoS ONE, 2015-06-22;10(6):e0130618. 2015-06-22 [PMID: 26098911] (Bioassay, Mouse)	Bioassay	Mouse
Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions. PLoS ONE, 2015-01-28;10(1):e0117908. 2015-01-28 [PMID: 25629162] (Bioassay, Mouse)	Bioassay	Mouse
Takenaka-Ninagawa , Nana, Kawabata , Yuka, Watanabe , Shogo, Nagata , Kohzo, Torihashi , Shigeko Generation of rat-induced pluripotent stem cells from a new model of metabolic syndrome. PLoS ONE, 2014-08-11;9(8):e104462. 2014-08-11 [PMID: 25111735] (Bioassay, Rat)	Bioassay	Rat
Rozycki M, Lodyga M, Lam J, Miranda M, Fatyol K, Speight P, Kapus A The fate of the primary cilium during myofibroblast transition. Mol Biol Cell, 2014-01-08;25(5):643-57. 2014-01-08 [PMID: 24403605] (Bioassay, Human)	Bioassay	Human
Au E, Ahmed T, Karayannis T, Biswas S, Gan L, Fishell G A modular gain-of-function approach to generate cortical interneuron subtypes from ES cells. Neuron, 2013-12-04;80(5):1145-58. 2013-12-04 [PMID: 24314726] (Bioassay, Mouse)	Bioassay	Mouse
Engevik A, Feng R, Yang L, Zavros Y The acid-secreting parietal cell as an endocrine source of Sonic Hedgehog during gastric repair. Endocrinology, 2013-10-03;154(12):4627-39. 2013-10-03 [PMID: 24092639] (Mouse)		Mouse
Liu , Xian Shu, Chopp , Michael, Wang , Xin Li, Zhang , Li, Hozeska-Solgot , Ann, Tang , Tao, Kassis , Haifa, Zhang , Rui Lan, Chen , Charles, Xu , Jennifer, Zhang , Zheng Ga MicroRNA-17-92 cluster mediates the proliferation and survival of neural progenitor cells after stroke. J Biol Chem, 2013-03-19;288(18):12478-88. 2013-03-19 [PMID: 23511639] (In Vivo, Mouse)	In Vivo	Mouse
Mills , Lisa D, Zhang , Yaqing, Marler , Ronald J, Herreros-Villanueva , Marta, Zhang , Lizhi, Almada , Luciana, Couch , Fergus, Wetmore , Cynthia, Pasca di Magliano , Marina, Fernandez-Zapico , Martin E Loss of the transcription factor GLI1 identifies a signaling network in the tumor microenvironment mediating KRAS oncogene-induced transformation. J Biol Chem, 2013-03-12;288(17):11786-94. 2013-03-12 [PMID: 23482563] (Bioassay, Mouse)	Bioassay	Mouse
Belgacem YH, Borodinsky LN Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord. Proc. Natl. Acad. Sci. U.S.A., 2011-02-28;108(11):4482-7. 2011-02-28 [PMID: 21368195] (Bioassay, Xenopus)	Bioassay	Xenopus
Del Debbio CB, Balasubramanian S, Parameswaran S, Chaudhuri A, Qiu F, Ahmad I Notch and Wnt signaling mediated rod photoreceptor regeneration by Muller cells in adult mammalian retina. PLoS ONE, 2010-08-26;5(8):e12425. 2010-08-26 [PMID: 20865053] (In Vivo, Rat)	In Vivo	Rat
Lipinski RJ, Bijlsma MF, Gipp JJ, Podhaizer DJ, Bushman W Establishment and characterization of immortalized Gli-null mouse embryonic fibroblast cell lines. BMC Cell Biol., 2008-09-13;9(0):49. 2008-09-13 [PMID: 18789160] (Bioassay, Mouse)	Bioassay	Mouse
Wan J, Zheng H, Xiao HL, She ZJ, Zhou GM Sonic hedgehog promotes stem-cell potential of Muller glia in the mammalian retina. Biochem. Biophys. Res. Commun., 2007-09-10;363(2):347-54. 2007-09-10 [PMID: 17880919] (Bioassay, In Vivo, Rat)	Bioassay, In Vivo	Rat
Salero E, Hatten ME Differentiation of ES cells into cerebellar neurons. Proc. Natl. Acad. Sci. U.S.A., 2007-02-09;104(8):2997-3002. 2007-02-09 [PMID: 17293457] (Bioassay, Mouse)	Bioassay	Mouse
Podlasek CA, Meroz CL, Tang Y, McKenna KE, McVary KT Regulation of cavernous nerve injury-induced apoptosis by sonic hedgehog. Biol. Reprod., 2006-09-20;76(1):19-28. 2006-09-20 [PMID: 16988214] (In Vivo, Rat)	In Vivo	Rat
Tiecke E, Bangs F, Blaschke R, Farrell ER, Rappold G, Tickle C Expression of the short stature homeobox gene Shox is restricted by proximal and distal signals in chick limb buds and affects the length of skeletal elements. Dev. Biol., 2006-07-12;298(2):585-96. 2006-07-12 [PMID: 16904661] (In Vivo, Chicken)	In Vivo	Chicken
Brito JM, Teillet MA, Le Douarin NM An early role for sonic hedgehog from foregut endoderm in jaw development: ensuring neural crest cell survival. Proc. Natl. Acad. Sci. U.S.A., 2006-07-25;103(31):11607-12. 2006-07-25 [PMID: 16868080] (Bioassay, Avian - Quail, Chicken)	Bioassay	Avian - Quail, Chicken
Yamashita H, Nakamura T, Takahashi T, Nagano Y, Hiji M, Hirabayashi T, Amano T, Yagi T, Sakai N, Kohriyama T, Matsumoto M Embryonic stem cell-derived neuron models of Parkinson's disease exhibit delayed neuronal death. J. Neurochem., 2006-07-01;98(1):45-56. 2006-07-01 [PMID: 16805795] (Bioassay, Mouse)	Bioassay	Mouse
Corti S, Locatelli F, Papadimitriou D, Donadoni C, Del Bo R, Fortunato F, Strazzer S, Salani S, Bresolin N, Comi GP Multipotentiality, homing properties, and pyramidal neurogenesis of CNS-derived LeX(ssea-1)+/CXCR4+ stem cells. FASEB J., 2005-09-08;19(13):1860-2. 2005-09-08 [PMID: 16150803] (Bioassay, Mouse)	Bioassay	Mouse
Perrier AL, Tabar V, Barberi T, Rubio ME, Bruses J, Topf N, Harrison NL, Studer L Derivation of midbrain dopamine neurons from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A., 2004-08-13;101(34):12543-8. 2004-08-13 [PMID: 15310843] (Bioassay, Human, Primate - Macaca mulatta (Rhesus Macaque))	Bioassay	Human, Primate - Macaca mulatta (Rhesus Macaque)
Abzhanov A, Tabin CJ Shh and Fgf8 act synergistically to drive cartilage outgrowth during cranial development. Dev. Biol., 2004-09-01;273(1):134-48. 2004-09-01 [PMID: 15302603] (Bioassay, Chicken)	Bioassay	Chicken
Jaskoll T, Witcher D, Toreno L, Bringas P, Moon AM, Melnick M FGF8 dose-dependent regulation of embryonic submandibular salivary gland morphogenesis. Dev. Biol., 2004-04-15;268(2):457-69. 2004-04-15 [PMID: 15063181] (Bioassay, Mouse)	Bioassay	Mouse
Viti J, Gulacsi A, Lillien L Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2. J. Neurosci., 2003-07-02;23(13):5919-27. 2003-07-02 [PMID: 12843296] (Bioassay, Mouse)	Bioassay	Mouse
Mistretta CM, Liu HX, Gaffield W, MacCallum DK Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium. Dev. Biol., 2003-02-01;254(1):1-18. 2003-02-01 [PMID: 12606278] (Bioassay, Rat)	Bioassay	Rat
Show All 36 Publications. Collapse Publications.

Array 461-SH

• Sonic Hedgehog/Shh Antibodies
• Sonic Hedgehog/Shh ELISA Kits
• Sonic Hedgehog/Shh Lysate
• Sonic Hedgehog/Shh Proteins
• Sonic Hedgehog/Shh Proteins and Enzymes
• Sonic Hedgehog/Shh ELISAs