Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

The Sonic Hedgehog (Shh) protein is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. The Shh protein regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue, Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor Shh protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). The Shh-C protein, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). The Shh-N protein is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of the Shh protein as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase the receptor binding affinity and signaling potency of the Shh-N protein (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of functionality of the Shh protein and are required for the development of Shh protein gradients important in tissue morphogenesis (9, 13). Canonical signaling by the Shh protein is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). Binding of the Shh protein to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 1 application: Bioassay.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using 8908-SH	Applications	Species
Schlissel, G;Meziane, M;Narducci, D;Hansen, AS;Li, P; Diffusion barriers imposed by tissue topology shape Hedgehog morphogen gradients Proceedings of the National Academy of Sciences of the United States of America 2024-09-03 [PMID: 39190357] (Bioassay, Mouse)	Bioassay	Mouse
Shi, P;Tian, J;Mallinger, JC;Ling, D;Deleyrolle, LP;McIntyre, JC;Caspary, T;Breunig, JJ;Sarkisian, MR; Increasing Ciliary ARL13B Expression Drives Active and Inhibitor-Resistant Smoothened and GLI into Glioma Primary Cilia Cells 2023-09-26 [PMID: 37830570] (Bioassay, Human)	Bioassay	Human
W Li, L Wang, BM Wierbowski, M Lu, F Dong, W Liu, S Li, P Wang, A Salic, X Gong Structural insights into proteolytic activation of the human Dispatched1 transporter for Hedgehog morphogen release Nature Communications, 2021-11-29;12(1):6966. 2021-11-29 [PMID: 34845226] (Bioassay, Human)	Bioassay	Human
FJ Gao, D Klinedinst, FX Fernandez, B Cheng, A Savonenko, B Devenney, Y Li, D Wu, MG Pomper, RH Reeves Forebrain Shh overexpression improves cognitive function and locomotor hyperactivity in an aneuploid mouse model of Down syndrome and its euploid littermates Acta neuropathologica communications, 2021-08-16;9(1):137. 2021-08-16 [PMID: 34399854] (Bioassay, Transgenic Mouse)	Bioassay	Transgenic Mouse
J Sun, DY Shin, M Eiseman, AR Yallowitz, N Li, S Lalani, Z Li, M Cung, S Bok, S Debnath, SJ Marquez, TE White, AG Khan, IC Lorenz, JH Shim, FS Lee, R Xu, MB Greenblatt SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts Nature Communications, 2021-07-29;12(1):4611. 2021-07-29 [PMID: 34326333] (Bioassay, Human)	Bioassay	Human
S Ichimiya, H Onishi, S Nagao, S Koga, K Sakihama, K Nakayama, A Fujimura, Y Oyama, A Imaizumi, Y Oda, M Nakamura GLI2 but not GLI1/GLI3 plays a central role in the induction of malignant phenotype of gallbladder cancer Oncology reports, 2021-01-22;45(3):997-1010. 2021-01-22 [PMID: 33650666] (Bioassay, Human)	Bioassay	Human
Y Xu, J Xi, G Wang, Z Guo, Q Sun, C Lu, L Ma, Y Wu, W Jia, S Zhu, X Guo, S Bian, J Kang PAUPAR and PAX6 sequentially regulate human embryonic stem cell cortical differentiation Nucleic Acids Research, 2021-02-26;0(0):. 2021-02-26 [PMID: 33544864] (Bioassay, Human)	Bioassay	Human
SM Griffin, MR Pickard, CP Hawkins, AC Williams, RA Fricker Nicotinamide restricts neural precursor proliferation to enhance catecholaminergic neuronal subtype differentiation from mouse embryonic stem cells PLoS ONE, 2020-09-14;15(9):e0233477. 2020-09-14 [PMID: 32925933] (Bioassay, Mouse)	Bioassay	Mouse
JW Kim, X Yin, A Jhaldiyal, MR Khan, I Martin, Z Xie, T Perez-Rose, M Kumar, L Abalde-Atr, J Xu, L Chen, SM Eacker, DJ Surmeier, NT Ingolia, TM Dawson, VL Dawson Defects in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium Homeostasis Cell Stem Cell, 2020-08-25;27(4):633-645.e7. 2020-08-25 [PMID: 32846140] (Bioassay, Human)	Bioassay	Human
X Qi, P Schmiege, E Coutavas, J Wang, X Li Structures of human Patched and its complex with native palmitoylated sonic hedgehog Nature, 2018-07-11;0(0):. 2018-07-11 [PMID: 29995851] (Bioassay, Human)	Bioassay	Human
N Kurio, C Saunders, TE Bechtold, I Salhab, HD Nah, S Sinha, PC Billings, M Pacifici, E Koyama Roles of Ihh signaling in chondroprogenitor function in postnatal condylar cartilage Matrix Biol., 2018-02-12;0(0):. 2018-02-12 [PMID: 29447948] (Bioassay, Mouse)	Bioassay	Mouse
Show All 11 Publications. Collapse Publications.

Array 8908-SH

• Sonic Hedgehog/Shh Antibodies
• Sonic Hedgehog/Shh ELISA Kits
• Sonic Hedgehog/Shh Lysate
• Sonic Hedgehog/Shh Proteins
• Sonic Hedgehog/Shh Proteins and Enzymes
• Sonic Hedgehog/Shh ELISAs