Recombinant Human Neuropilin-1 Fc Avi-tag Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Neuropilin-1 (NRP1), also known as CD304, is a type I transmembrane glycoprotein involved in embryonic development, axon guidance and vasculature formation (1‑4). The Neuropilin family is comprised of two isoforms, NRP1 and NRP2, that arose due to gene duplication and share 44% amino acid (aa) sequence identity (1). The extracellular domain (ECD) of Neuropilins are structurally similar containing two N‑terminal CUB domains (termed a1/a2), two domains with homology to coagulation factors V and VIII (b1/b2) and a MAM (meprin) domain (5). The ECD of NRP1 shares 93% aa sequence identity with mouse and rat NRP1. Several C‑terminally divergent splice variants of NRP1 and NRP2 lacking the MAM or transmembrane domain have been identified (5, 6). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). They form homo- and hetero-oligomers via interaction of their MAM domains and share overlapping sets of ligands and functions (1, 7). Both Neuropilins act as co-receptors with Plexins and Semaphorins to help mediate axon repulsion (8, 9). NRP1 preferentially binds SEMA3A and PLXNA4, while NRP2 prefers binding to SEMA3F and PLXNA3 (8‑10). NRP1 is capable of binding VEGF and forming signaling complexes with VEGFR2 to mediate cell migration (10, 11). There is evidence that NRP1 with VEGF plays a role in stemness of breast cancer cell by activating Wnt/b-catenin pathway (12). Additionally, NRP1 is expressed on dendritic cells and mediates DC‑induced T cell proliferation (13). NRP1 is a marker of CD4+ Treg cells and a population of CD8+ T-cells infiltrating solid tumors and immunotherapies blocking NRP1 synergize with anti-PD1 to enhance CD8+ proliferation and response (14). NRP1 has been shown to bind the S1 fragment of the SARS-CoV-2 spike protein and may potentially provide a therapeutic target for COVID-19 (15). Our Avi-tag Biotinylated NRP1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Array AVI10455

• Neuropilin-1 Antibodies
• Neuropilin-1 Lysates
• Neuropilin-1 Protein
• Neuropilin-1 Proteins and Enzymes
• Neuropilin-1 ELISAs