Recombinant Human NELL1 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

NELL1 (neural EGF‑like like protein 1) is an approximately 140 kDa modular glycoprotein that plays an important role in bone physiology (1). NELL1 contains an N‑terminal Laminin G‑like domain and three vWF‑C domains interspersed with five tandem EGF‑like domains (2). Mature human NELL1 shares 93% aa identity with mouse and rat NELL1. Alternative splicing of human NELL1 generates a short isoform that lacks the fourth EGF‑like domain. NELL1 can be retained in the cytosol where it interacts with ARP3 (apoptosis‑related protein 3) and becomes phosphorylated by PKC (3, 4). NELL1 is secreted as an approximately 400 kDa noncovalent homotrimer of heavily glycosylated subunits (5). It is expressed in bone and in select B cell lines (6, 7). NELL1 promotes the osteogenic differentiation of adipose‑derived stromal/stem cells and inhibits adipogenic differentiation (8). It does not promote osteoblastic differentiation from myoblasts, but it does enhance the activity of BMP‑2 in that function (9). NELL1 interacts directly with osteoblasts via Integrin alpha 3 beta 1 (10, 11) and promotes osteoblast differentiation and mineralization (3, 12, 13). It synergizes with BMP‑2 to increase phosphate uptake through the transporters Pit1 and Pit2 in pre‑osteoblasts (14). In vivo, NELL1 expression in the cranium is localized at sites of suture closure (6). Its over‑expression induces multiple abnormalities in cranial development including premature suture closure (craniosyntosis) and overlapping sutures (12, 13). Experimental engrafting demonstrates the ability of NELL1 to promote new bone formation and the healing of calvarial defects (13, 15).

We have publications tested in 2 confirmed species: Human, Virus.

We have publications tested in 2 applications: Bioassay, Surface Plasmon Resonance (SPR.

Showing Publications 1 - 2 of 2.

Publications using 5487-NL	Applications	Species
X Huang, X Cen, B Zhang, Y Liao, Z Zhao, G Zhu, Z Zhao, J Liu The roles of circRFWD2 and circINO80 during NELL-1-induced osteogenesis J. Cell. Mol. Med., 2019-10-21;0(0):. 2019-10-21 [PMID: 31633307] (Bioassay, Human)	Bioassay	Human
Martinez-Martin N, Marcandalli J, Huang C, Arthur C, Perotti M, Foglierini M, Ho H, Dosey A, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor. Cell, 2018-07-26;174(5):1158-1171.e19. 2018-07-26 [PMID: 30057110] (Surface Plasmon Resonance (SPR, Virus)	Surface Plasmon Resonance (SPR	Virus

Array 5487-NL

• NELL1 Antibodies
• NELL1 ELISA Kits
• NELL1 Protein
• NELL1 Proteins and Enzymes