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Recombinant Human LRP-4 Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human LRP-4 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human USAG1 (Catalog # 5370-SD) is coated at 2 μg/mL, Recombinant Human LRP-4 binds with an apparent Kd <1 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human LRP-4 protein
Human LRP-4
(Ser21-Ser1725)
Accession # AAI36669		 GGGSGGGSGGGS  6-His
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser21 & Gly212
Protein/Peptide Type
Recombinant Proteins
Gene
LRP4
Purity
>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
191 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
180-225 kDa, reducing conditions
Publications
Read Publications using
5948-LR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRP-4 Protein, CF

  • CLSS
  • KIAA0816
  • low density lipoprotein receptor-related protein 4
  • low-density lipoprotein receptor-related protein 4
  • LRP10
  • LRP4
  • LRP-4
  • MEGF7LRP10
  • Multiple epidermal growth factor-like domains 7

Background

LDL receptor-related protein 4 (LRP-4), also known as Megf7, is an approximately 220 kDa type I membrane protein in the LDL receptor superfamily. LRP-4 plays an important role in development of the neuromuscular junction (NMJ), bones, teeth, mammary placodes, and hair follicles (1, 2). Mature human LRP-4 consists of a 1705 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 159 aa cytoplasmic domain. The ECD contains 3 EGF-like domains, 8 LDLR-A domains, and 20 LDLR-B repeats that fold into 4 beta-propeller domains (3, 4). Within the ECD, human LRP-4 shares 97% aa sequence identity with mouse and rat LRP-4. The ECD of LRP-4 can be shed by ADAM10 mediated cleavage (5). LRP-4 is expressed on myotubes (6, 7), neurons (8, 9), osteocytes and osteoblasts (10, 11), developing tooth epithelium (12), and male and female germ cells (13). It is localized to NMJ on developing myotubes, and it associates in cis with the muscle-specific kinase MuSK (6, 7). Its binding to neuron-derived Agrin promotes additional LRP-4 association with MuSK, MuSK activation, clustering of AChR, and post-synaptic development of the NMJ (6, 7, 14). LRP-4 also enhances presynaptic differentiation of the NMJ, although this does not require Agrin (14). Neuronal LRP-4 additionally binds ApoE and F-spondin (8, 9). LRP-4 regulates both BMP and Wnt signaling through binding to Wise, Sclerostin, and Dkk1 (10-12). These interactions are important for the control of bone growth and mineralization, tooth morphogenesis, and the development of mammary placodes, hair follicles, and whiskers (10-12, 15).
  1. Zong, Y. and R. Jin (2012) Cell. Mol. Life Sci. Nov 22 Epub.
  2. Ohazama, A. et al. (2010) Am. J. Med. Genet. A 152A:2974.
  3. Nakayama, M. et al. (1998) Genomics 51:27.
  4. Tomita, Y. et al. (1998) J. Biochem. 124:784.
  5. Dietrich, M.F. et al. (2010) PLoS ONE 5:e9960.
  6. Kim, N. et al. (2008) Cell 135:334.
  7. Zhang, B. et al. (2008) Neuron 60:285.
  8. Lu, Y. et al. (2007) Brain Res. 1177:19.
  9. Zisman, S. et al. (2007) J. Cell Biol. 178:1237.
  10. Leupin, O. et al. (2011) J. Biol. Chem. 286:19489.
  11. Choi, H.Y. et al. (2009) PLoS ONE 4:e7930.
  12. Ohazama, A. et al. (2008) PLoS ONE 3:e4092.
  13. Yamaguchi, Y.L. et al. (2006) Gene Expr. Patterns 6:607.
  14. Yumoto, N. et al. (2012) Nature 489:438.
  15. Ahn, Y. et al. (2013) Development 140:583.

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Publications for LRP-4 (5948-LR)(2)

We have publications tested in 2 confirmed species: Human, Xenopus.

We have publications tested in 1 application: Bioassay.


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(2)
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Bioinformatics

Gene Symbol LRP4
Uniprot