Recombinant Human His6- (DYKDDDDK)-p53 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

p53 is well known for its key role as a tumor suppressor protein.  It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73 (1,2). Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation (3). p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis, including  cell cycle regulatory proteins and members the Bcl-2 family (3). Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination (3-5). A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation (6,7).

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.

Showing Publications 1 - 3 of 3.

Publications using SP-452	Applications	Species
J Li, Y Wang, Y Luo, Y Liu, Y Yi, J Li, Y Pan, W Li, W You, Q Hu, Z Zhao, Y Zhang, Y Cao, L Zhang, J Yuan, ZJ Xiao USP5-Beclin 1 axis overrides p53-dependent senescence and drives Kras-induced tumorigenicity Nature Communications, 2022;13(1):7799. 2022 [PMID: 36528652] (Bioassay, Human)	Bioassay	Human
J Li, Y Wang, Y Luo, Y Liu, Y Yi, J Li, Y Pan, W Li, W You, Q Hu, Z Zhao, Y Zhang, Y Cao, L Zhang, J Yuan, ZJ Xiao USP5-Beclin 1 axis overrides p53-dependent senescence and drives Kras-induced tumorigenicity Nature Communications, 2022-12-17;13(1):7799. 2022-12-17 [PMID: 36528652] (Bioassay, Human)	Bioassay	Human
K Bhuripanyo, Y Wang, X Liu, L Zhou, R Liu, D Duong, B Zhao, Y Bi, H Zhou, G Chen, NT Seyfried, WJ Chazin, H Kiyokawa, J Yin Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer Sci Adv, 2018-01-03;4(1):e1701393. 2018-01-03 [PMID: 29326975] (Bioassay)	Bioassay

Array SP-452

• p53 Antibodies
• p53 Antibody Pair
• p53 ELISA Kits
• p53 Kit
• p53 Lysates
• p53 Proteins
• p53 ELISAs
• p53 Proteins and Enzyme